219 results match your criteria γ-gcs

Granulosa cells affect in vitro maturation and subsequent parthenogenetic development of buffalo (Bubalus bubalis) oocytes.

Reprod Domest Anim 2021 May 31. Epub 2021 May 31.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.

Granulosa cells (GCs) play a crucial role in follicular development and atresia. Previous studies have showed that GCs in the form of monolayer influenced in vitro maturation (IVM) of oocytes. However, the effects of GCs in the form of conditioned medium and monolayer on IVM and development competence of buffalo oocytes remain unclear. Read More

View Article and Full-Text PDF

Effect of Decompressive Hemicraniectomy in Patients with Acute Middle Cerebral Artery Infarction.

Turk J Med Sci 2021 Apr 23. Epub 2021 Apr 23.

Aim: We aimed to determine in which cases this procedure may be more effective based on the data of patients who underwent decompressive hemicraniectomy (DHC).

Material And Methods: Overall, 47 patients who underwent DHC due to acute middle cerebral artery (MCA) infarction between January 2014 and january 2019 were retrospectively investigated. These patients were divided into two groups: those who died after DHC (Group A) and those who survived DHC (Group B). Read More

View Article and Full-Text PDF

AQP8 participates in oestrogen-mediated buffalo follicular development by regulating apoptosis of granulosa cells.

Reprod Domest Anim 2021 May 9;56(5):812-820. Epub 2021 Mar 9.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.

Aquaporins (AQPs), a family of small membrane-spanning proteins, are involved in fluid transport, cell signalling and reproduction. Regulating AQP8 expression influences apoptosis of granulosa cells (GCs), ovarian folliculogenesis, oogenesis and early embryonic development in mice, but its role has never been investigated in other species. The aim of the present study was to characterize the AQP8 function in buffalo follicular development. Read More

View Article and Full-Text PDF

Increased c-di-GMP Levels Lead to the Production of Alginates of High Molecular Mass in Azotobacter vinelandii.

J Bacteriol 2020 11 19;202(24). Epub 2020 Nov 19.

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico

produces the linear exopolysaccharide alginate, a compound of significant biotechnological importance. The biosynthesis of alginate in and has several similarities but is regulated somewhat differently in the two microbes. Here, we show that the second messenger cyclic dimeric GMP (c-di-GMP) regulates the production and the molecular mass of alginate in The hybrid protein MucG, containing conserved GGDEF and EAL domains and N-terminal HAMP and PAS domains, behaved as a c-di-GMP phosphodiesterase (PDE). Read More

View Article and Full-Text PDF
November 2020

Tyrosine-phosphorylation and activation of glucosylceramide synthase by v-Src: Its role in survival of HeLa cells against ceramide.

Biochim Biophys Acta Mol Cell Biol Lipids 2021 01 25;1866(1):158817. Epub 2020 Sep 25.

Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan.

Sphingolipids represent a family of cellular lipid-molecules that regulate physiological and pathophysiological processes. Glucosylceramide (GlcCer), the simplest glycosphingolipid (GSL), is synthesized from ceramide and UDP-glucose by GlcCer synthase (GCS). Both GlcCer (and resulting GSLs) and ceramide regulate various cellular functions including cell death and multiple drug resistance. Read More

View Article and Full-Text PDF
January 2021

Altered m A modification is involved in up-regulated expression of FOXO3 in luteinized granulosa cells of non-obese polycystic ovary syndrome patients.

J Cell Mol Med 2020 10 1;24(20):11874-11882. Epub 2020 Sep 1.

Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

The pathophysiology of polycystic ovary syndrome (PCOS) is characterized by granulosa cell (GC) dysfunction. m A modification affects GC function in patients with premature ovarian insufficiency (POI), but the role of m A modification in PCOS is unknown. The purpose of the prospective comparative study was to analyse the m A profile of the luteinized GCs from normovulatory women and non-obese PCOS patients following controlled ovarian hyperstimulation. Read More

View Article and Full-Text PDF
October 2020

Theca cell-conditioned medium enhances steroidogenesis competence of buffalo (Bubalus bubalis) granulosa cells.

Reprod Domest Anim 2021 Feb 10;56(2):254-262. Epub 2020 Dec 10.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.

Theca cells (TCs) play a crucial role in follicular development and atresia. TCs synthesize androgens that act as substrate for granulosa cells (GCs) aromatization to oestrogens needed for follicular growth. However, the effects of TCs in the form of conditioned medium on steroidogenesis in buffalo GCs remain unclear. Read More

View Article and Full-Text PDF
February 2021

B-Cell Lymphoma 2 (Bcl-2) Gene Is Associated with Intracranial Hypertension after Severe Traumatic Brain Injury.

J Neurotrauma 2021 01 10;38(2):291-299. Epub 2020 Aug 10.

Department of Neurological Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.

Severe traumatic brain injury (TBI) activates the apoptotic cascade in neurons and glia as part of secondary cellular injury. B-cell lymphoma 2 () gene encodes a pro-survival protein to suppress programmed cell death, and variation in this gene has potential to affect intracranial pressure (ICP). Participants were recruited from a single clinical center using a prospective observational study design. Read More

View Article and Full-Text PDF
January 2021

Prosthetic aortic graft replacement of the ascending thoracic aorta alters biomechanics of the native descending aorta as assessed by transthoracic echocardiography.

PLoS One 2020 12;15(3):e0230208. Epub 2020 Mar 12.

Department of Medicine (Cardiology), Weill Cornell Medicine, New York, New York, United States of America.

Introduction: In patients with ascending aortic (AA) aneurysms, prosthetic graft replacement yields benefit but risk for complications in the descending aorta persists. Longitudinal impact of AA grafts on native descending aortic physiology is poorly understood.

Methods: Transthoracic echocardiograms (echo) in patients undergoing AA elective surgical grafting were analyzed: Descending aortic deformation indices included global circumferential strain (GCS), time to peak (TTP) strain, and fractional area change (FAC). Read More

View Article and Full-Text PDF

Tranexamic acid in traumatic brain injury: an explanatory study nested within the CRASH-3 trial.


Eur J Trauma Emerg Surg 2021 Feb 19;47(1):261-268. Epub 2020 Feb 19.

Purpose: The CRASH-3 trial is a randomised trial of tranexamic acid (TXA) on death and disability in patients with traumatic brain injury (TBI). It is based on the hypothesis that early TXA treatment can prevent deaths from post-traumatic intracranial bleeding. The results showed that timely TXA treatment reduces head injury deaths in patients with reactive pupils and those with a mild to moderate GCS at baseline. Read More

View Article and Full-Text PDF
February 2021

First attempt success of video versus direct laryngoscopy for endotracheal intubation by ambulance nurses: a prospective observational study.

Eur J Trauma Emerg Surg 2020 Oct 19;46(5):1039-1045. Epub 2020 Feb 19.

Trauma Research Unit Department of Surgery, Erasmus MC, University Medical Center Rotterdam, P.O. Box 2040, 3000 CA, Rotterdam, The Netherlands.

Purpose: The aim of this study was to compare the rate of first attempt success of endotracheal intubation performed by ambulance nurses in patients with a Glasgow Coma Scale (GCS)  of 3 using video laryngoscopy versus direct laryngoscopy.

Methods: A prospective cohort study was conducted in a single, independent ambulance service. Twenty of a total of 65 nurse-staffed ambulances were equipped with a video laryngoscope; a classic direct laryngoscope (Macintosh) was available on all 65 ambulances. Read More

View Article and Full-Text PDF
October 2020

π-Helix controls activity of oxygen-sensing diguanylate cyclases.

Biosci Rep 2020 02;40(2)

Department of Biochemistry and Molecular Biology, Pennsylvania State University, 306 Althouse Laboratory, University Park, PA 16802, U.S.A.

The ability of organisms to sense and adapt to oxygen levels in their environment leads to changes in cellular phenotypes, including biofilm formation and virulence. Globin coupled sensors (GCSs) are a family of heme proteins that regulate diverse functions in response to O2 levels, including modulating synthesis of cyclic dimeric guanosine monophosphate (c-di-GMP), a bacterial second messenger that regulates biofilm formation. While GCS proteins have been demonstrated to regulate O2-dependent pathways, the mechanism by which the O2 binding event is transmitted from the globin domain to the cyclase domain is unknown. Read More

View Article and Full-Text PDF
February 2020

Adrenal suppression from glucocorticoids: preventing an iatrogenic cause of morbidity and mortality in children.

BMJ Paediatr Open 2019 23;3(1):e000569. Epub 2019 Oct 23.

Pediatrics, University of Ottawa, Ottawa, Ontario, Canada.

Adrenal suppression (AS) is an important side effect of glucocorticoids (GCs) including inhaled corticosteroids (ICS). AS can often be asymptomatic or associated with non-specific symptoms until a physiological stress such as an illness precipitates an adrenal crisis. Morbidity and death associated with adrenal crisis is preventable but continues to be reported in children. Read More

View Article and Full-Text PDF
October 2019

Association Between DNA and RNA Oxidative Damage and Mortality of Patients with Traumatic Brain Injury.

Neurocrit Care 2020 06;32(3):790-795

Department of Neurosurgery, Hospital, Universitario de Canarias, Ofra, s/n. La Laguna, 38320, Santa Cruz de Tenerife, Spain.

Background: The hyperoxidative state in traumatic brain injury (TBI) could produce oxidative damage on the ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). Oxidative damage to nucleic acids in TBI patients has been studied, and higher concentrations of 8-OHdG were found in postmortem brain samples of subjects who died following TBI than in subjects who died from sudden cardiac death. Thus, the objective of this study was to determine whether there is an association between serum DNA and RNA oxidative damage and mortality in TBI patients. Read More

View Article and Full-Text PDF

Quercetin protects human granulosa cells against oxidative stress via thioredoxin system.

Reprod Biol 2019 Sep 3;19(3):245-254. Epub 2019 Aug 3.

Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Pour Sina St, Tehran, 1417613151, Iran; Department of Infertility, Shariati Hospital, Tehran University of Medical Sciences, Tehran, 1411713135, Iran. Electronic address:

Granulosa Cells (GCs) are sensitive to excessive production of reactive oxygen species (ROS). Quercetin (QUR) is a free radical scavenger which can alleviate oxidative stress through nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/antioxidant response element (ARE) pathway and thioredoxin (Trx) system. We aimed to explore the probable protective role of QUR on cultured human GCs treated with hydrogen peroxide (HO) as an inducer of oxidative stress. Read More

View Article and Full-Text PDF
September 2019

Budesonide use and misuse in sports: Elimination profiles of budesonide and metabolites after intranasal, high-dose inhaled and oral administrations.

Drug Test Anal 2020 May 9;12(5):629-636. Epub 2019 Aug 9.

IMIM (Hospital del Mar Medical Research Institute), Doping Control Research Group, Catalonian Antidoping Laboratory, Barcelona, Spain.

Budesonide (BUD) is a glucocorticoid (GC) widely used in therapeutics. In sports, the World Anti-doping Agency (WADA) controls the use of GCs, and WADA-accredited laboratories use a reporting level of 30 ng/mL for 6β-hydroxy-budesonide (6βOHBUD) to detect the systemic administration of BUD. In the present work, we examined the urinary excretion profile of 6βOHBUD, BUD, and 16α-hydroxy-prednisolone (16αOHPRED) after intranasal (INT), inhaled (INH) (at high doses) and oral administrations in male and female volunteers. Read More

View Article and Full-Text PDF

Elevated cerebrospinal fluid concentrations of N-acetylaspartate correlate with poor outcome in a pilot study of severe brain trauma.

Brain Inj 2019 15;33(10):1364-1371. Epub 2019 Jul 15.

g School of Nursing, University of Pittsburgh , Pittsburgh , Pennsylvania , USA.

: Examine the correlation between acute cerebrospinal fluid (CSF) levels of N-acetylaspartate (NAA) and injury severity upon admission in addition to long-term functional outcomes of severe traumatic brain injury (TBI). : This exploratory study assessed CSF NAA levels in the first four days after severe TBI, and correlated these findings with Glasgow Coma Scale (GCS) score and long-term outcomes at 3, 6, 12, and 24 months post-injury. : CSF was collected after passive drainage via an indwelling ventriculostomy placed as standard of care in a total of 28 people with severe TBI. Read More

View Article and Full-Text PDF

Measurement of 11-dehydrocorticosterone in mice, rats and songbirds: Effects of age, sex and stress.

Gen Comp Endocrinol 2019 09 27;281:173-182. Epub 2019 May 27.

Department of Zoology, University of British Columbia, Vancouver, BC, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, BC, Canada; Department of Psychology, University of British Columbia, Vancouver, BC, Canada; Graduate Program in Neuroscience, University of British Columbia, Vancouver, BC, Canada. Electronic address:

Glucocorticoids (GCs) are secreted into the blood by the adrenal glands and are also locally-produced by organs such as the lymphoid organs (bone marrow, thymus, and spleen). Corticosterone is the primary circulating GC in many species, including mice, rats and birds. Within lymphoid organs, corticosterone can be locally produced from the inactive metabolite, 11-dehydrocorticosterone (DHC). Read More

View Article and Full-Text PDF
September 2019

The Effect of Continuous Renal Replacement Therapy with the AN69ST Membrane on Inflammatory Markers and the Level of Consciousness of Hemodialysis Patients with Stroke: Comparison with Hemodialysis with Low Blood Flow Rate.

Pril (Makedon Akad Nauk Umet Odd Med Nauki) 2018 Dec;39(2-3):29-35

Cardiovascular Center, Hanwa Memorial Hospital, 7-11-11 Karita, Sumiyoshi-ku,Osaka 558-0011, Japan.

Introduction: Hemodialysis (HD) with low blood flow rate, continuous renal replacement therapy (CRRT), and peritoneal dialysis are recommended for patients with stroke complications to prevent intracranial hypertension because of the low diffusion capacity of the brain barrier. However, detailed guidelines are not currently available; thus, there is an urgent need to establish such guidelines.

Material And Methods: We developed a novel protocol for performing CRRT with the AN69ST membrane, which has excellent adsorption capacity for various cytokines. Read More

View Article and Full-Text PDF
December 2018

Protective effects of ethyl gallate on HO-induced mitochondrial dysfunction in PC12 cells.

Metab Brain Dis 2019 04 12;34(2):545-555. Epub 2019 Feb 12.

Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, 44 Wenhuaxi Road, Jinan, 250012, People's Republic of China.

Oxidative stress has been suggested to play an important role in neuronal injury. Ethyl gallate (EG) is the ethyl ester of gallic acid which has been acknowledged as an antioxidant. We previously demonstrated that EG effectively inhibited HO-induced cytotoxicity and decreased the ROS levels in PC12 cells, while the relevant mechanisms of action of this compound remain largely uncharacterized. Read More

View Article and Full-Text PDF

Use of echocardiography to monitor myocardial damage during anthracycline chemotherapy.

Echocardiography 2019 03 12;36(3):495-502. Epub 2019 Jan 12.

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China.

Background: Anthracycline-related cardiotoxicity has a poor prognosis; therefore, early detection of any change in LV function is critical.

Objective: The aim of this study was to evaluate the two-dimensional speckle tracking technique for the early detection of cardiac toxicity after low-dose anthracycline chemotherapy in the Chinese population.

Methods: Forty breast cancer patients were treated by chemotherapy using anthracycline for 4-6 cycles. Read More

View Article and Full-Text PDF

Effectiveness of Injectable Antibiotics on the Recovery of Acute -Infected Pigs.

Microb Drug Resist 2019 May 18;25(4):603-610. Epub 2018 Dec 18.

1 Clinic of Medicine, Faculty of Veterinary Medicine, School of Health Sciences, University of Thessaly, Karditsa, Greece.

The aim of this trial was to evaluate the effectiveness of injectable antibiotics of one- or two-dose administration on recovery of acute App ()-infected pigs. Ninety pigs with moderate general clinical score (GCS) of a commercial farm, suffering from acute App infection, were divided in two groups: (a) T1: one administration of gamithromycin injectable solution and (b) T2: two administrations of florfenicol injectable solution. Morbidity/mortality, clinical scores (clinical appearance score index-CAS, clinical respiratory score-CRS, clinical cough score index [CCS], general respiratory clinical score-GCRS, and general clinical score-GCS), body temperature score (BTS), and posttreatment interval were recorded. Read More

View Article and Full-Text PDF

Ethyl 2,4-dicarboethoxy pantothenate, a derivative of pantothenic acid, prevents cellular damage initiated by environmental pollutants through Nrf2 activation.

J Dermatol Sci 2018 Nov 9;92(2):162-171. Epub 2018 Sep 9.

School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakuracho, Hachiouji, Tokyo 192-0982, Japan. Electronic address:

Background: Recently, environmental pollutants have become a concern not only for respiratory organs but also for skin-related human health, because skin is localized at the border between the human body and the external environment and is easily influenced by environmental pollutants.

Objective: Here, we investigated the effects of a novel pantothenic acid (PA) derivative, ethyl 2,4-dicarboethoxy pantothenate (EDCEP), on a diesel particulate extract (DPE) as a representative environmental pollutant that generates reactive oxygen species (ROS) through the activation of aryl hydrocarbon receptor (AHR) signaling.

Methods: We characterized the effects of PA and EDCEP on normal human epidermal keratinocytes (NHEKs) exposed to DPE or HO as a general oxidative stress stimulator. Read More

View Article and Full-Text PDF
November 2018

The effect of anesthetic agents on cerebral vasospasms after subarachnoid hemorrhage: A retrospective study.

Medicine (Baltimore) 2018 Aug;97(31):e11666

Department of Anesthesiology and Pain Medicine Department of Neurosurgery, College of Medicine, Ewha Womans University, Seoul, Korea.

Cerebral vasospasm is the most important cause of morbidity after an aneurysm clipping in the early postoperative period. The aim of this retrospective study was to evaluate whether the incidence of vasospasms differs when using propofol or desflurane for an emergent aneurysm clipping.The data from 102 patients (50 in the propofol group, 52 in the desflurane group) were analyzed. Read More

View Article and Full-Text PDF

Effect of hyperbaric oxygen combined with nimodipine on treatment of diffuse brain injury.

Exp Ther Med 2018 Jun 10;15(6):4651-4658. Epub 2018 Apr 10.

Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 05000, P.R. China.

The aim of the present study was to was investigate the treatment efficacy of hyperbaric oxygen (HBO) combined with nimodipine on diffuse brain injury. AA total of 80 patients with diffuse brain injury were randomly divided into four groups: Group A, conventional treatment; Group B, conventional treatment + nimodipine; Group C, conventional treatment + HBO therapy and Group D, conventional treatment + nimodipine + HBO therapy. The Glasgow Coma Scale (GCS) score and serum tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels were assessed before treatment and at 8, 24, 48 and 72 h after treatment. Read More

View Article and Full-Text PDF

Low-dose dexamethasone affects osteoblast viability by inducing autophagy via intracellular ROS.

Mol Med Rep 2018 Mar 18;17(3):4307-4316. Epub 2018 Jan 18.

Department of Sports Medicine, The First Hospital of China Medical University, Shenyang, Liaoning 110000, P.R. China.

Glucocorticoids (GCs) are closely associated with the progression of GC‑induced osteoporosis (GIOP) by inhibiting osteoblast viability. However, endogenous GCs are important for bone development. In addition, previous studies have demonstrated that GCs could induce autophagy, a cytoprotective process that is protective against various stressors. Read More

View Article and Full-Text PDF

Early Seizure Prophylaxis in Traumatic Brain Injuries Revisited: A Prospective Observational Study.

World J Surg 2018 06;42(6):1727-1732

Los Angeles County + University of Southern California (LAC + USC) Medical Center, 2051 Marengo Street, Inpatient Tower (C), Rm C5L100, Los Angeles, CA, 90033, USA.

Introduction: Pharmacological prophylaxis for early seizures following traumatic brain injury (TBI) is a recommendation in the Brain Trauma Foundation Guidelines. However, several studies have challenged the efficacy and safety of this practice, resulting in varied practice across centers around the world. The purpose of the present study was to compare the incidence of early clinical seizures following TBI, between two large centers, a US Center that practises routine seizure prophylaxis and a Chinese Center that does not use seizure prophylaxis following TBI. Read More

View Article and Full-Text PDF

Involvement of bilitranslocase and beta-glucuronidase in the vascular protection by hydroxytyrosol and its glucuronide metabolites in oxidative stress conditions.

J Nutr Biochem 2018 01 3;51:8-15. Epub 2017 Oct 3.

LaPEC EA4278, University of Avignon, F-84000 Avignon, France. Electronic address:

Olive oil vascular benefits have been attributed to hydroxytyrosol (HT). However, HT biological actions are still debated because it is extensively metabolized into glucuronides (GCs). The aim of this study was to test HT and GC vasculoprotective effects and the underlying mechanisms using aorta rings from 8-week-old male Wistar rats. Read More

View Article and Full-Text PDF
January 2018

Activation of Nrf2 might reduce oxidative stress in human granulosa cells.

Mol Cell Endocrinol 2018 07 4;470:96-104. Epub 2017 Oct 4.

Department of Obstetrics and Gynecology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113 8655, Japan.

Nuclear factor-E2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1)-antioxidant response element (ARE) signaling pathway is one of the most important defense mechanisms against oxidative stress (OS). It is well documented that equilibration status of OS plays fundamental roles in human reproductive medicine, and the physiological role of Nrf2 in ovarian granulosa cells (GCs) has not been determined yet. Herein we aimed to study the function of Nrf2 in GCs. Read More

View Article and Full-Text PDF

A Decision Instrument to Identify Isolated Traumatic Subdural Hematomas at Low Risk of Neurologic Deterioration, Surgical Intervention, or Radiographic Worsening.

Acad Emerg Med 2017 11 23;24(11):1377-1386. Epub 2017 Oct 23.

Department of Emergency Medicine, Massachusetts General Hospital, Boston, MA.

Objectives: Subdural hematoma (SDH) is the most common form of traumatic intracranial hemorrhage. Severity of disease in patients with SDH varies widely. It was hypothesized that a decision rule could identify patients with SDH who are at very low risk for neurologic decline, neurosurgical intervention, or radiographic worsening. Read More

View Article and Full-Text PDF
November 2017