4,499 results match your criteria γ-aminobutyric acid

Effect of forage type, season, and ripening time on selected quality properties of sheep milk cheese.

J Dairy Sci 2021 Mar 15;104(3):2539-2552. Epub 2021 Jan 15.

Department of Food Hygiene and Technology, Faculty of Veterinary Science, University of León, 24071, León, Spain. Electronic address:

The aim of this research was to study changes in the microbial populations, free AA profile, biogenic amine content, and sensory characteristics of ripened cheeses (100 and 180 d) produced in different seasons (summer, autumn, winter, and spring) from pasteurized sheep milk from 8 commercial flocks fed hay or silage diets. Twenty-one individual AA and 6 biogenic amines were determined by ultra-high performance liquid chromatography. Type of conserved forage for sheep feeding did not affect the variables studied, which is of great interest because hay and silage are low-cost ingredients for sheep feeding. Read More

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Development and validation of a UPLC-MS/MS method for the simultaneous determination of gamma-aminobutyric acid and glutamic acid in human plasma.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Feb 28;1164:122519. Epub 2020 Dec 28.

Division of Human Nutrition and Health, Wageningen University & Research, P.O. Box 17, 6700 AA Wageningen, the Netherlands.

Gamma-aminobutyric acid (GABA) and its precursor glutamic acid are important neurotransmitters. Both are also present in peripheral tissues and the circulation, where abnormal plasma concentrations have been linked to specific mental disorders. In addition to endogenous synthesis, GABA and glutamic acid can be obtained from dietary sources. Read More

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February 2021

Pyridoxal kinase inhibition by artemisinins down-regulates inhibitory neurotransmission.

Proc Natl Acad Sci U S A 2020 12 14;117(52):33235-33245. Epub 2020 Dec 14.

Institute of Structural Biology, Rudolf Virchow Center for Integrative and Translational Bioimaging, University of Würzburg, 97080 Würzburg, Germany;

The antimalarial artemisinins have also been implicated in the regulation of various cellular pathways including immunomodulation of cancers and regulation of pancreatic cell signaling in mammals. Despite their widespread application, the cellular specificities and molecular mechanisms of target recognition by artemisinins remain poorly characterized. We recently demonstrated how these drugs modulate inhibitory postsynaptic signaling by direct binding to the postsynaptic scaffolding protein gephyrin. Read More

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December 2020

Prognostic Role of Serum Amino Acids in Head and Neck Cancer.

Dis Markers 2020 1;2020:2291759. Epub 2020 Oct 1.

Dipartimento di Scienze dell'Invecchiamento, Neurologiche, Ortopediche e della Testa-Collo, Fondazione Policlinico Universitario A. Gemelli IRCCS, Largo Gemelli 8, I-00168 Rome, Italy.

Introduction: Serum amino acid (AA) profiles represent a valuable tool in the metabolic assessment of cancer patients; still, information on the AA pattern in head and neck cancer (HNC) patients is insufficient. The aim of the study was to assess whether serum AA levels were associated with the stage of neoplastic disease and prognosis in primary HNC patients.

Methods: Two hundred and two primary HNC patients were included in the study. Read More

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October 2020

Multicenter Study to Transplant Hepatitis C-Infected Kidneys (MYTHIC): An Open-Label Study of Combined Glecaprevir and Pibrentasvir to Treat Recipients of Transplanted Kidneys from Deceased Donors with Hepatitis C Virus Infection.

J Am Soc Nephrol 2020 11 25;31(11):2678-2687. Epub 2020 Aug 25.

Department of Biostatistics, Epidemiology and Informatics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania.

Background: Single-center trials and retrospective case series have reported promising outcomes using kidneys from donors with hepatitis C virus (HCV) infection. However, multicenter trials are needed to determine if those findings are generalizable.

Methods: We conducted a prospective trial at seven centers to transplant 30 kidneys from deceased donors with HCV viremia into HCV-uninfected recipients, followed by 8 weeks of once-daily coformulated glecaprevir and pibrentasvir, targeted to start 3 days posttransplant. Read More

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November 2020

Antiepileptic combination therapy with Stevens-Johnson syndrome and toxic epidermal necrolysis: Analysis of a Japanese pharmacovigilance database.

Epilepsia 2020 09 6;61(9):1979-1989. Epub 2020 Aug 6.

Laboratory of Clinical Pharmacy, Gifu Pharmaceutical University, Gifu, Japan.

Objective: Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are immune-mediated diseases characterized by an extensive loss of the epidermal skin layer, often resulting in death. SJS and TEN are often triggered by certain drugs, including antiepileptic drugs (AEDs). Epilepsy is very difficult to treat and often involves the combination of two or more AEDs. Read More

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September 2020

Three-dimensional high-performance liquid chromatographic analysis of chiral amino acids in carbonaceous chondrites.

J Chromatogr A 2020 Aug 31;1625:461255. Epub 2020 May 31.

Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higasi-ku, Fukuoka 812-8582, Japan. Electronic address:

A three-dimensional (3D) HPLC system in combination with fluorescence derivatization has been developed for the highly sensitive and selective analysis of chiral amino acids in extraterrestrial samples. As the targets, alanine (Ala), 2-aminobutyric acid (2AB), valine (Val), norvaline (nVal) and isovaline (iVal), frequently found chiral amino acids in the carbonaceous chondrites, were selected. These amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and the target analytes were separated from other amino acids and organic compounds by a reversed-phase column in the first dimension. Read More

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A Thorough QT Study of the Combination Glecaprevir + Pibrentasvir on Cardiac Repolarization in Healthy Subjects.

Clin Ther 2020 07 1;42(7):1317-1329. Epub 2020 Jul 1.

Clinical Pharmacology and Pharmacometrics, USA. Electronic address:

Purpose: Fixed-dose combination glecaprevir (GLE) 300 mg + pibrentasvir (PIB) 120 mg is an orally administered once daily antiviral regimen approved for the treatment of hepatitis C virus (HCV) infection. The objective of this study was to evaluate the potential for cardiac repolarization following GLE + PIB administration in healthy adults.

Methods: This placebo- and active-controlled, randomized, single-dose, 4-period, 4-sequence crossover study enrolled 48 healthy subjects. Read More

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Determination of glyphosate, AMPA and glufosinate by high performance liquid chromatography with fluorescence detection in waters of the Santarém Plateau, Brazilian Amazon.

J Environ Sci Health B 2020 25;55(9):794-802. Epub 2020 Jun 25.

Laboratory of Toxicology, Faculty of Health Sciences, University of Brasília, Brasília, DF, Brazil.

Herbicide use, mainly glyphosate, has been intense in worldwide agriculture, including in the Brazilian Amazon region. This study aimed to validate a method for determining glyphosate and its degradation product, AMPA, and glufosinate by HPLC-FL in 58 water samples collected at the Santarém plateau region (), in the western of Pará state, Brazil. The method involves filtration and direct injection in the HPLC-FL for AMPA analysis, or previous concentration (10×) by lyophilization for glufosinate and glyphosate analysis. Read More

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September 2020

Mutually stabilizing interactions between proto-peptides and RNA.

Nat Commun 2020 06 19;11(1):3137. Epub 2020 Jun 19.

NSF/NASA Center for Chemical Evolution, Atlanta, GA, USA.

The close synergy between peptides and nucleic acids in current biology is suggestive of a functional co-evolution between the two polymers. Here we show that cationic proto-peptides (depsipeptides and polyesters), either produced as mixtures from plausibly prebiotic dry-down reactions or synthetically prepared in pure form, can engage in direct interactions with RNA resulting in mutual stabilization. Cationic proto-peptides significantly increase the thermal stability of folded RNA structures. Read More

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Effects of Open-Label, Adjunctive Ganaxolone on Persistent Depression Despite Adequate Antidepressant Treatment in Postmenopausal Women: A Pilot Study.

J Clin Psychiatry 2020 06 9;81(4). Epub 2020 Jun 9.

Neuroendocrine Unit, BUL 457B, Massachusetts General Hospital, Boston, MA 02114.

Objective: The neuroactive steroid metabolite of progesterone, allopregnanolone, is a positive allosteric modulator of γ-aminobutyric acid-A (GABAA) receptors and a putative treatment for mood disorders. This pilot study was performed to determine whether an oral allopregnanolone analog (ganaxolone) may be effective adjunctive therapy for persistent depression despite adequate antidepressant treatment in postmenopausal women.

Method: Ten postmenopausal women (mean ± SD age: 62. Read More

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Photoaffinity labeling identifies an intersubunit steroid-binding site in heteromeric GABA type A (GABA) receptors.

J Biol Chem 2020 08 15;295(33):11495-11512. Epub 2020 Jun 15.

Department of Neurobiology, Harvard Medical School, Boston, Massachusetts

Allopregnanolone (3α5α-P), pregnanolone, and their synthetic derivatives are potent positive allosteric modulators (PAMs) of GABA receptors (GABARs) with anesthetic, anxiolytic, and anti-convulsant effects. Mutational analysis, photoaffinity labeling, and structural studies have provided evidence for intersubunit and intrasubunit steroid-binding sites in the GABAR transmembrane domain, but revealed only little definition of their binding properties. Here, we identified steroid-binding sites in purified human α1β3 and α1β3γ2 GABARs by photoaffinity labeling with [H]21-[4-(3-(trifluoromethyl)-3H-diazirine-3-yl)benzoxy]allopregnanolone ([H]21-TFDBzox-AP), a potent GABAR PAM. Read More

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Differential Pulse Polarographic Investigations on Glyphosate and Glufosinate Herbicides in Relation to Their Environmental Analysis.

Bull Environ Contam Toxicol 2020 Jul 14;105(1):95-102. Epub 2020 Jun 14.

Department of Chemistry, Himachal Pradesh University, Shimla, 171005, India.

Differential pulse polarographic (DPP) investigations on the reaction of the amino function of glyphosate and glufosinate herbicides with carbon disulphide and copper(II) perchlorate forming copper(III) dithiocarbamate complexes were made in the presence of sodium perchlorate in acetonitrile at dropping mercury electrode (DME). The newly formed herbicide complexes exhibited analytically useful diffusion-controlled peaks at - 115 mV and - 110 mV versus saturated calomel electrode (SCE) with linear relationship between current and concentration. This observation formed the basis for the determination of glyphosate and glufosinate in the concentration ranges 0. Read More

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Nicotinamide Mononucleotide Administration Prevents Experimental Diabetes-Induced Cognitive Impairment and Loss of Hippocampal Neurons.

Int J Mol Sci 2020 May 26;21(11). Epub 2020 May 26.

Department of Neurology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Diabetes predisposes to cognitive decline leading to dementia and is associated with decreased brain NAD levels. This has triggered an intense interest in boosting nicotinamide adenine dinucleotide (NAD) levels to prevent dementia. We tested if the administration of the precursor of NAD, nicotinamide mononucleotide (NMN), can prevent diabetes-induced memory deficits. Read More

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Synthesis and nucleic acid binding evaluation of a thyminyl l-diaminobutanoic acid-based nucleopeptide.

Bioorg Chem 2020 07 18;100:103862. Epub 2020 Apr 18.

Istituto di Biostrutture e Bioimmagini IBB - CNR, Via Mezzocannone 16, 80134 Naples, Italy. Electronic address:

Herein we present the synthesis of a l-diaminobutanoic acid (DABA)-based nucleopeptide (3), with an oligocationic backbone, realized by solid phase peptide synthesis using thymine-bearing DABA moieties alternating in the sequence with free ones. CD studies evidenced the ability of this oligothymine nucleopeptide, well soluble in aqueous solution, to alter the secondary structure particularly of complementary RNA (poly rA vs poly rU) and inosine-rich RNAs, like poly rI and poly rIC, and showed its preference in binding double vs single-stranded DNAs. Furthermore, ESI mass spectrometry revealed that 3 bound also G-quadruplex (G4) DNAs, with either parallel or antiparallel topologies (adopted in our experimental conditions by c-myc and tel, respectively). Read More

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Reduced cadmium accumulation in tobacco by sodium chloride priming.

Environ Sci Pollut Res Int 2020 Oct 12;27(30):37410-37418. Epub 2020 May 12.

Institute of Tobacco Research, Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao, 266101, People's Republic of China.

Cadmium (Cd) pollution threatens agricultural security worldwide. This study tested the efficacy of priming chemicals to decrease Cd uptake by tobacco plants (Nicotiana tabacum). After initial screening from nine different chemicals (NaCl, Cd(CHCOO), Cd(NO), CdCl, KHNO, polyethylene glycol 6000 (PEG-6000), indole-3-acetic acid (IAA), ß-aminobutyric acid (BABA), and glutathione (GSH)), NaCl and PEG-6000 were further investigated because of their low risks to plant growth and efficiency to Cd reduction. Read More

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October 2020

Prescription analgesia and adjuvant use by pain severity at admission among nursing home residents with non-malignant pain.

Eur J Clin Pharmacol 2020 Jul 3;76(7):1021-1028. Epub 2020 May 3.

Department of Population and Quantitative Health Science, University of Massachusetts Medical School, 368 Plantation Street, Worcester, MA, 01605, USA.

Objective: We estimated the use of prescribed analgesics and adjuvants among nursing home residents without cancer who reported pain at their admission assessment, in relation to resident-reported pain severity.

Methods: Medicare Part D claims were used to define 3 classes of analgesics and 7 classes of potential adjuvants on the 21st day after nursing home admission (or the day of discharge for residents discharged before that date) among 180,780 residents with complete information admitted between January 1, 2011 and December 9, 2016, with no cancer diagnosis.

Results: Of these residents, 27. Read More

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Design, Synthesis, and Insecticidal Activity of Novel Doramectin Derivatives Containing Acylurea and Acylthiourea Based on Hydrogen Bonding.

J Agric Food Chem 2020 May 13;68(21):5806-5815. Epub 2020 May 13.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, People's Republic of China.

Our recent investigation on the insecticidal activities of several doramectin derivatives preliminarily revealed that the presence of hydrogen bonds at the C4″ position of the molecule with target protein γ-aminobutyric acid (GABA) receptor was crucial for retaining high insecticidal activity. As a continuation of our research work on the development of new insecticides, two series of novel acylurea and acylthiourea doramectin derivatives were designed and synthesized. The bioassay results indicated that the newly synthesized compounds (, , and ) exhibited higher insecticidal activity against diamondback moth, oriental armyworm, and corn borer than the control compounds doramectin, commercial avermectins, chlorbenzuron, and lead compound in our laboratory. Read More

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Dynorphin and GABAA Receptor Signaling Contribute to Progesterone's Inhibition of the LH Surge in Female Mice.

Endocrinology 2020 05;161(5)

Department of Women and Children's Health, Faculty of Life Sciences and Medicine, King's College London, Guy's Campus, UK.

Progesterone can block estrogen-induced luteinising hormone (LH) surge secretion and can be used clinically to prevent premature LH surges. The blocking effect of progesterone on the LH surge is mediated through its receptor in the anteroventral periventricular nucleus (AVPV) of the hypothalamus. However, the underlying mechanisms are unclear. Read More

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Efficacy of Gabapentin for the Treatment of Alcohol Use Disorder in Patients With Alcohol Withdrawal Symptoms: A Randomized Clinical Trial.

JAMA Intern Med 2020 05;180(5):728-736

Addiction Sciences Division, Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston.

Importance: Although an estimated 30 million people meet criteria for alcohol use disorder (AUD), few receive appropriate pharmacotherapy. A more personalized, symptom-specific, approach might improve efficacy and acceptance.

Objective: To examine whether gabapentin would be useful in the treatment of AUD, especially in those with the most alcohol withdrawal symptoms. Read More

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Effects of Gaba Derivatives on Anxious and Compulsive Behavior in Offspring of Rats with Experimental Preeclampsia.

Bull Exp Biol Med 2020 Feb 7;168(4):457-464. Epub 2020 Mar 7.

Department of Pharmacology and Biopharmacia, Faculty of Continuing Medical Education, Volgograd State Medical University, Ministry of Health of the Russian Federation, Volgograd, Russia.

We studied the effects of GABA derivatives on anxious and compulsive behavior of progeny of rats with experimental preeclampsia provoked by replacement of drinking water for 1.8% NaCl solution from the first day of pregnancy to delivery. In comparison with progeny of health rats, the offspring of dams with complicated pregnancy demonstrated high level of anxiety and the development of obsessive-compulsive disorder both at the early (40 and 70 days) and late (6 and 12 months) stages of ontogeny. Read More

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February 2020

Nociception in a Progressive Multiple Sclerosis Model in Mice Is Dependent on Spinal TRPA1 Channel Activation.

Mol Neurobiol 2020 May 24;57(5):2420-2435. Epub 2020 Feb 24.

Graduated Program in Pharmacology, Federal University of Santa Maria (UFSM), Avenida Roraima, Santa Maria, RS, 97105-900, Brazil.

Central neuropathic pain is a common untreated symptom in progressive multiple sclerosis (PMS) and is associated with poor quality of life and interference with patients' daily activities. The neuroinflammation process and mitochondrial dysfunction in the PMS lesions generate reactive species. The transient potential receptor ankyrin 1 (TRPA1) has been identified as one of the major mechanisms that contribute to neuropathic pain signaling and can be activated by reactive compounds. Read More

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Determination of glyphosate and glufosinate in corn using multi-walled carbon nanotubes followed by ultra high performance liquid chromatography coupled with tandem mass spectrometry.

J Chromatogr A 2020 May 8;1619:460939. Epub 2020 Feb 8.

Sanitary Inspection Institute, Tianjin Centers for Disease Control and Prevention, 6 Huayue Road, Hedong District, Tianjin 300011, PR China. Electronic address:

Glyphosate (Glyp) and glufosinate (Gluf) are widely used herbicides around the world, and their effects on human health and detection of levels have drawn increasing attention. The present study was to establish a method to determine the contents of Glyp and Gluf from corn using multi-walled carbon nanotubes (MWCNTs) followed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The corn samples were purified by MWCNTs, then the analytes reacted with 9-fluorenylmethylchloroformate (FMOCCl) of acetonitrile solution (20. Read More

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The Role of the Central Amygdala in Alcohol Dependence.

Cold Spring Harb Perspect Med 2021 Feb 1;11(2). Epub 2021 Feb 1.

Departments of Molecular Medicine and Neuroscience, The Scripps Research Institute, La Jolla, California 92037, USA.

Alcohol dependence is a chronically relapsing disorder characterized by compulsive drug-seeking and drug-taking, loss of control in limiting intake, and the emergence of a withdrawal syndrome in the absence of the drug. Accumulating evidence suggests an important role for synaptic transmission in the central nucleus of the amygdala (CeA) in mediating alcohol-related behaviors and neuroadaptive mechanisms associated with alcohol dependence. Acute alcohol facilitates γ-aminobutyric acid (GABA)ergic transmission in the CeA via both pre- and postsynaptic mechanisms, and chronic alcohol increases baseline GABAergic transmission. Read More

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February 2021

Simultaneous edited MRS of GABA, glutathione, and ethanol.

NMR Biomed 2020 04 14;33(4):e4227. Epub 2020 Jan 14.

Department of Clinical and Health Psychology, University of Florida, Gainesville, FL, USA.

The aim of this work was to develop simultaneous edited MRS of γ-aminobutyric acid (GABA), glutathione (GSH), and ethanol (EtOH) using Hadamard encoding and reconstruction of MEGA-edited spectroscopy (HERMES) at 3T. Density-matrix simulations of HERMES were carried out and compared with phantom experiments. In vivo experiments were performed in six healthy volunteers about 30 min after alcohol consumption. Read More

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Thalamic Gamma Aminobutyric Acid Level Changes in Major Depressive Disorder After a 12-Week Iyengar Yoga and Coherent Breathing Intervention.

J Altern Complement Med 2020 Mar 14;26(3):190-197. Epub 2020 Jan 14.

Department of Psychiatry, Boston University School of Medicine, Boston, MA.

To determine if a 12-week yoga intervention (YI) was associated with increased gamma aminobutyric acid (GABA) levels and decreased depressive symptoms in participants with major depressive disorder (MDD). : Subjects were randomized to a high-dose group (HDG) of three YIs a week and a low-dose group (LDG) of two YIs a week. Thalamic GABA levels were obtained using magnetic resonance spectroscopy at Scan-1 before randomization. Read More

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Influence of Lactobacillus brevis on metabolite changes in bacteria-fermented sufu.

J Food Sci 2020 Jan 3;85(1):165-172. Epub 2020 Jan 3.

Beijing Laboratory for Food Quality and Safety, College of Food Science & Nutritional Engineering, China Agricultural Univ., Beijing, China.

Sufu is a form of food derived from traditional Chinese fermented soybean. It has a unique flavor and contains abundant nutrients. With demands for healthy food on the rise, a higher level of sufu functionality is required. Read More

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January 2020

Magnetic resonance spectroscopy across chronic pain disorders: a systematic review protocol synthesising anatomical and metabolite findings in chronic pain patients.

Syst Rev 2019 12 27;8(1):338. Epub 2019 Dec 27.

Trinity College Institute of Neuroscience, Trinity College Dublin, Lloyd Building, Dublin 2, Ireland.

Background: Chronic pain is pain greater than 3 months duration that may result from disease, trauma, surgery, or unknown origin. The overlap between the psychological, behavioural, and management aspects of pain suggest that limbic brain neurochemistry plays a role in chronic pain pathology. Proton magnetic resonance spectroscopy (H-MRS) can evaluate in vivo brain metabolites including creatine, N-acetylaspartate, myo-inositol, choline, glutamate, glutamine, and gamma-aminobutyric acid in chronic pain; however, a comprehensive systemic review of metabolite expression patterns across all brain areas has yet to be performed. Read More

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December 2019

Pleiotropic actions of allopregnanolone underlie therapeutic benefits in stress-related disease.

Neurobiol Stress 2020 May 27;12:100203. Epub 2019 Nov 27.

Department of Psychiatry, Department of Pharmacology, Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill, School of Medicine, Chapel Hill, NC, 27599, USA.

For several years, research from around the world has suggested that the neuroactive steroid (3α,5α)-3-hydroxypregnan-20-one (allopregnanolone) may have therapeutic potential for treatment of various stress-related diseases including post-traumatic stress disorder (PTSD), depression, alcohol use disorders (AUDs), as well as neurological and psychiatric conditions that are worsened in the presence of stress, such as multiple sclerosis, schizophrenia, and seizure disorders. In this review, we make the argument that the pleiotropic actions of allopregnanolone account for its ability to promote recovery in such a wide variety of illnesses. Likewise, the allopregnanolone precursors, pregnenolone and progesterone, share many actions of allopregnanolone. Read More

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