17,367 results match your criteria αΑ66-80 aggregation

Platelet Reactivity and Response to Aspirin and Clopidogrel in Patients with Platelet Count Disorders.

Cardiol Res Pract 2021 17;2021:6637799. Epub 2021 Apr 17.

Department of Hematology, Blood Neoplasms, and Bone Marrow Transplantation, Wroclaw Medical University, Wrocław, Poland.

Background: Platelet reactivity and response to antiplatelet drugs, acetylsalicylic acid (ASA) and clopidogrel, in patients with thrombocytopenia and thrombocythemia can have a potentially important effect on the outcome. The effectiveness and safety of antiplatelet drugs in such patients has not been well examined. Measuring the effect of ASA and clopidogrel on platelets could help guide the therapy. Read More

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Methods to study the structure of misfolded protein states in systemic amyloidosis.

Biochem Soc Trans 2021 Apr 30. Epub 2021 Apr 30.

Institute of Protein Biochemistry, Ulm University, 89081 Ulm, Germany.

Systemic amyloidosis is defined as a protein misfolding disease in which the amyloid is not necessarily deposited within the same organ that produces the fibril precursor protein. There are different types of systemic amyloidosis, depending on the protein constructing the fibrils. This review will focus on recent advances made in the understanding of the structural basis of three major forms of systemic amyloidosis: systemic AA, AL and ATTR amyloidosis. Read More

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[The mechanism of HDAC6 in paraquat-induced autophagy dysfunction of dopaminergic neurons by mediating aggresome-autophagy-lysosomal pathway].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2021 Apr;39(4):241-247

Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health and Management, Ningxia Medical University, Yinchuan 750004, China.

To explore the mechanism of HDAC6 mediated aggresome-autophagy-lysosome pathway in paraquat-induced autophagy in dopaminergic neurons. Human neuroblastoma cell (SH-SY5Y cell) was used as model of dopaminergic neurons in vitro. The cells were treated with terminal concentrations of 0, 25, 50, 100, 200 and 400μmol/L PQ for 24 hours, and the cells were induced by 100 μmol/L PQ for different time (0, 12, 24, 36, 48, 60 and 72 h) . Read More

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Interference of C6O4 on platelet aggregation pathways: Cues on the new-generation of perfluoro-alkyl substance.

Environ Int 2021 Apr 23;154:106584. Epub 2021 Apr 23.

Department of Medicine and Unit of Andrology and Reproduction Medicine, University of Padova, Padova, Italy. Electronic address:

Background: Health concerns associated with the exposure to legacy perfluoro-alkyl substances (PFAS) led to the development of new-generation PFAS, such as C6O4. Here we investigated the possible effects of C6O4 on the platelet's activation profile, by incubating human platelets from healthy donors with C6O4 at different concentrations and evaluating the effects on activation, production and phenotype of platelets micro-particles (MPV) and aggregation under-flow. Based on the eventual platelet pro-aggregation profile detected, the preventive effect of acetylsalicylic acid (ASA) was also explored. Read More

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The effect of warfarin administration on platelet aggregation.

Bratisl Lek Listy 2021 ;122(5):320-324

Background: The aim of this study was to assess the effect of anticoagulation treatment on platelet aggregation.

Methods: The study group consisted of 24 patients on long-term warfarin therapy without any antiaggregation therapy. Platelet aggregation was measured using VerifyNow with arachidonic acid (AA) as an inducer in 23 patients and with light transmission aggregometry (LTA) in 19 patients using four different agonists. Read More

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Stronger inhibitory effects of Ticagrelor plus aspirin compared with Clopidogrel plus aspirin on arachidonic acid-induced platelet aggregation in patients with acute coronary syndrome with PCI.

J Pak Med Assoc 2021 02;71(2(A)):540-542

Department of Cardiology, First Affiliated Hospital, Dalian Medical University, Dalian, China.

Antagonists of the Adenosine Diphosphate (ADP) receptor, P2Y12, may inhibit platelet aggregation as a result of stimulation with arachidonic acid (AA). The potent P2Y12 blocker, Ticagrelor has greater anti-platelet effects than Clopidogrel. We explored the effects of Ticagrelor versus Clopidogrel on mean maximum aggregation ratios (MAR%) in response to AA stimulation in patients receiving aspirin in conventional doses. Read More

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February 2021

Evaluation of the ATN model in a longitudinal memory clinic sample with different underlying disorders.

Alzheimers Dement (Amst) 2021 1;13(1):e12031. Epub 2021 Apr 1.

Department of Psychiatry and Neurochemistry Sahlgrenska Academy Institute of Neuroscience and Physiology University of Gothenburg Sweden.

Introduction: To evaluate the usefulness of the 2018 NIA-AA (National Institute on Aging and Alzheimer's Association) research framework in a longitudinal memory clinic study with different clinical outcomes and underlying disorders.

Methods: We included 420 patients with mild cognitive impairment or subjective cognitive impairment. During the follow up, 27% of the patients converted to dementia, with the majority converting to Alzheimer's disease (AD) or mixed dementia. Read More

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Overconsumption of Omega-6 Polyunsaturated Fatty Acids (PUFAs) versus Deficiency of Omega-3 PUFAs in Modern-Day Diets: The Disturbing Factor for Their "Balanced Antagonistic Metabolic Functions" in the Human Body.

J Lipids 2021 17;2021:8848161. Epub 2021 Mar 17.

Department of Biology, College of Natural and Computational Science, Debre Berhan University, P.O. Box 445, Debre Berhan, Ethiopia.

Polyunsaturated fatty acids (PUFAs) contain ≥2 double-bond desaturations within the acyl chain. Omega-3 (n-3) and Omega-6 (n-6) PUFAs are the two known important families in human health and nutrition. In both Omega families, many forms of PUFAs exist: -linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) from the n-3 family and linoleic acid (LA), dihomo--linolenic acid (DGLA), and arachidonic acid (AA) from the n-6 family are the important PUFAs for human health. Read More

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Adenosine Receptor Agonist HE-NECA Enhances Antithrombotic Activities of Cangrelor and Prasugrel in vivo by Decreasing of Fibrinogen Density in Thrombus.

Int J Mol Sci 2021 Mar 17;22(6). Epub 2021 Mar 17.

Department of Haemostasis and Haemostatic Disorders, Chair of Biomedical Sciences, Medical University of Lodz, Mazowiecka 6/8, 92-235 Lodz, Poland.

Blood platelets' adenosine receptors (AR) are considered to be a new target for the anti-platelet therapy. This idea is based on in vitro studies which show that signaling mediated by these receptors leads to a decreased platelet response to activating stimuli. In vivo evidence for the antithrombotic activity of AR agonists published to date were limited, however, to the usage of relatively high doses given in bolus. Read More

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The Role of Z-disc Proteins in Myopathy and Cardiomyopathy.

Int J Mol Sci 2021 Mar 17;22(6). Epub 2021 Mar 17.

Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK.

The Z-disc acts as a protein-rich structure to tether thin filament in the contractile units, the sarcomeres, of striated muscle cells. Proteins found in the Z-disc are integral for maintaining the architecture of the sarcomere. They also enable it to function as a (bio-mechanical) signalling hub. Read More

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Gain of toxic function by long-term AAV9-mediated SMN overexpression in the sensorimotor circuit.

Nat Neurosci 2021 Apr 1. Epub 2021 Apr 1.

Center for Motor Neuron Biology and Disease, Columbia University, New York, NY, USA.

The neurodegenerative disease spinal muscular atrophy (SMA) is caused by deficiency in the survival motor neuron (SMN) protein. Currently approved SMA treatments aim to restore SMN, but the potential for SMN expression beyond physiological levels is a unique feature of adeno-associated virus serotype 9 (AAV9)-SMN gene therapy. Here, we show that long-term AAV9-mediated SMN overexpression in mouse models induces dose-dependent, late-onset motor dysfunction associated with loss of proprioceptive synapses and neurodegeneration. Read More

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Treatment of COVID-19: a review of current and prospective pharmacotherapies.

Br J Hosp Med (Lond) 2021 Mar 12;82(3):1-9. Epub 2021 Mar 12.

Department of Intensive Care, Barnet Hospital, Royal Free London NHS Foundation Trust, London, UK.

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread and have grave health and socioeconomic consequences worldwide. Researchers have raced to understand the pathophysiological mechanisms underpinning the disease caused by SARS-CoV-2 so that effective therapeutic targets can be discovered. This review summarises the key pharmacotherapies that are being investigated for treatment of COVID-19, including antiviral, immunomodulator and anticoagulation strategies. Read More

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NAC blocks Cystatin C amyloid complex aggregation in a cell system and in skin of HCCAA patients.

Nat Commun 2021 03 23;12(1):1827. Epub 2021 Mar 23.

The Center for Applied Genomics, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Hereditary cystatin C amyloid angiopathy is a dominantly inherited disease caused by a leucine to glutamine variant of human cystatin C (hCC). L68Q-hCC forms amyloid deposits in brain arteries associated with micro-infarcts, leading ultimately to paralysis, dementia and death in young adults. To evaluate the ability of molecules to interfere with aggregation of hCC while informing about cellular toxicity, we generated cells that produce and secrete WT and L68Q-hCC and have detected high-molecular weight complexes formed from the mutant protein. Read More

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Weighting or aggregating? Investigating information processing in multi-attribute choices.

Health Econ 2021 Mar 19. Epub 2021 Mar 19.

Health Economics Research Unit, University of Aberdeen, Aberdeen, UK.

Multi-attribute choices are commonly analyzed in economics to value goods and services. Analysis assumes individuals consider all attributes, making trade-offs between them. Such decision-making is cognitively demanding, often triggering alternative decision rules. Read More

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complex formation of human PYRIN domain-only protein 3 prevented by self-oligomerization of ASC PYD domain.

Saudi J Biol Sci 2021 Mar 5;28(3):1607-1614. Epub 2021 Jan 5.

Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

The formation of inflammasome complexes contributes inactivation of inflammatory caspases caspase 1, which is generally considered essential for the innate response. Three proteins constituted this inflammasome complex, such as Nod-like receptors (NLRP or AIM2), ASC possessing caspase-recruiting domain, and caspase-1. The ASC proteins comprise two domains, the N-terminal PYD domain responsible for the interaction of various proteins, including PYD only protein 3 (POP3), and the CARD domain for association with other proteins. Read More

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Acridone and acridinium constructs with red-shifted emission.

Methods Appl Fluoresc 2021 Mar 23;9(2):025006. Epub 2021 Mar 23.

Applied Research and Technology, Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, Illinois 60064, United States of America.

Acridinium 9-carboxylic acid derivatives have been extensively used as chemiluminescent labels in diagnostic assays. Triggering acridinium with basic hydrogen peroxide produces a highly strained dioxetanone intermediate, which converts into an acridone in an electronically excited state and emits light at 420-440 nm. Here, we introduce a novel acridinium-fluorescein construct emitting at 530 nm, in which fluorescein is covalently attached to the acridinium N-10 nitrogen via a propyl sulfonamide linker. Read More

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Turn-on fluorescence measurement of acid phosphatase activity through an aggregation-induced emission of thiolate-protected gold nanoclusters.

Talanta 2021 May 9;227:122197. Epub 2021 Feb 9.

The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou, 215123, China. Electronic address:

A fluorescence method was developed for a turn-on measurement of acid phosphatase (ACP) activity. It was found that cerous ion (Ce) could lead to an enhancement of glutathione protected gold nanocluster fluorescence through an aggregation-induced-emission (AIE) process, while its higher valent counterpart ceric ion (Ce) could not. In a weakly acidic environment, ACP catalyzed the dephosphorylation of a phosphate ester of ascorbic acid, with a generation of ascorbic acid (AA). Read More

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Isolation of Extracellular Vesicles from Biological Fluids via the Aggregation-Precipitation Approach for Downstream miRNAs Detection.

Diagnostics (Basel) 2021 Feb 24;11(3). Epub 2021 Feb 24.

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, Russia.

Extracellular vesicles (EVs) have high potential as sources of biomarkers for non-invasive diagnostics. Thus, a simple and productive method of EV isolation is demanded for certain scientific and medical applications of EVs. Here we aim to develop a simple and effective method of EV isolation from different biofluids, suitable for both scientific, and clinical analyses of miRNAs transported by EVs. Read More

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February 2021

Methylselenol Produced In Vivo from Methylseleninic Acid or Dimethyl Diselenide Induces Toxic Protein Aggregation in .

Int J Mol Sci 2021 Feb 24;22(5). Epub 2021 Feb 24.

Laboratoire de Biologie Structurale de la Cellule, BIOC, École Polytechnique, CNRS-UMR7654, IP Paris, 91128 Palaiseau CEDEX, France.

Methylselenol (MeSeH) has been suggested to be a critical metabolite for anticancer activity of selenium, although the mechanisms underlying its activity remain to be fully established. The aim of this study was to identify metabolic pathways of MeSeH in to decipher the mechanism of its toxicity. We first investigated in vitro the formation of MeSeH from methylseleninic acid (MSeA) or dimethyldiselenide. Read More

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February 2021

Arachidonic acid-induced platelet aggregation and acetylsalicylic acid treatment during pregnancy in women with recurrent miscarriage, a post hoc study.

Platelets 2021 Mar 1:1-7. Epub 2021 Mar 1.

Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

In this post hoc study, arachidonic acid (AA)-induced platelet aggregation during pregnancy with and without acetylsalicylic acid (ASA) treatment was studied in 323 women with unexplained recurrent first-trimester miscarriage and in 59 healthy women with normal pregnancies. All women had normal AA-induced platelet aggregation in the non-pregnant state. Women with recurrent miscarriage were treated with 75 mg ASA or placebo daily. Read More

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Mesothelin blockage by Amatuximab suppresses cell invasiveness, enhances gemcitabine sensitivity and regulates cancer cell stemness in mesothelin-positive pancreatic cancer cells.

BMC Cancer 2021 Feb 26;21(1):200. Epub 2021 Feb 26.

Department of Gastroenterological Surgery I, Hokkaido University Graduate School of Medicine, North 15, West 7, Kita-Ku, Sapporo, Hokkaido, 060-8638, Japan.

Background: Mesothelin is a 40-kDa glycoprotein that is highly overexpressed in various types of cancers, however molecular mechanism of mesothelin has not been well-known. Amatuximab is a chimeric monoclonal IgG1/k antibody targeting mesothelin. We recently demonstrated that the combine therapy of Amatuximab and gemcitabine was effective for peritonitis of pancreatic cancer in mouse model. Read More

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February 2021

Accurate and fast mitotic detection using an anchor-free method based on full-scale connection with recurrent deep layer aggregation in 4D microscopy images.

BMC Bioinformatics 2021 Feb 26;22(1):91. Epub 2021 Feb 26.

Research Center for Healthcare Data Science, Zhejiang Lab, Hangzhou, China.

Background: To effectively detect and investigate various cell-related diseases, it is essential to understand cell behaviour. The ability to detection mitotic cells is a fundamental step in diagnosing cell-related diseases. Convolutional neural networks (CNNs) have been successfully applied to object detection tasks, however, when applied to mitotic cell detection, most existing methods generate high false-positive rates due to the complex characteristics that differentiate normal cells from mitotic cells. Read More

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February 2021

Aggregation Behavior of Poly(Acrylic acid-co-Octadecyl Methacrylate) and Bovine Serum Albumin in Aqueous Solutions.

ChemistryOpen 2021 Mar 25;10(3):373-379. Epub 2021 Feb 25.

School of Materials Science and Engineering, Hebei University of Technology, 300130, Tianjin, China.

Polymer-protein complexing systems have been extensively studied because of their wide application in biomedicine and industry. Here, we studied the aggregation behavior of the hydrophobically associating water-soluble polymer poly(acrylic acid-co-octadecyl methacrylate) [P(AA-co-OMA)] prepared with nonionic surfactant as an emulsifier and bovine serum albumin (BSA) in aqueous solution. We identified the optimal composite conditions of P(AA-co-OMA) and BSA aqueous solution. Read More

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Interaction of Aromatic Amino Acid-Functionalized Gold Nanoparticles with Lipid Bilayers: Insight into the Emergence of Novel Lipid Corona Formation.

J Phys Chem B 2021 03 19;125(8):2113-2123. Epub 2021 Feb 19.

Discipline of Chemistry, Indian Institute of Technology Indore, Indore 453552, Madhya Pradesh, India.

The coating of proteins and lipids around the surface of the nanoparticles is known as "protein corona" and "lipid corona", respectively, which have promising biomedical applications. While protein corona formation is well-known, the lipid corona is relatively new and its stability is yet to be explored. In the present contribution, we report a novel lipid corona formation and its underlying mechanism using aromatic amino acid-functionalized gold nanoparticles (Au-AA NPs) as a template by means of spectroscopic (steady-state UV-visible and fluorescence) and imaging (CLSM, HR-TEM, and AFM) techniques. Read More

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Response to antiplatelet therapy in patients undergoing invasive treatment due to acute coronary syndrome complicated by cardiogenic shock.

Postepy Kardiol Interwencyjnej 2020 Dec 29;16(4):418-421. Epub 2020 Dec 29.

Department and Clinic of Cardiology, Wroclaw Medical University, Wroclaw, Poland.

Introduction: There are limited data on platelet reactivity and response to antiplatelet drugs in patients with cardiogenic shock.

Aim: To assess platelet reactivity on dual antiplatelet therapy with acetylsalicylic acid (ASA) and ticagrelor, a novel potent P2Y12 receptor inhibitor, in patients with cardiogenic shock in the course of acute coronary syndrome (ACS) who received invasive treatment.

Material And Methods: We enrolled 12 consecutive patients with ACS complicated by cardiogenic shock. Read More

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December 2020

Clinical Post-operative Bleeding During Minor Oral Surgical Procedure and In Vitro Platelet Aggregation in Patients on Aspirin Therapy: Are they Coherent?

J Maxillofac Oral Surg 2021 Mar 20;20(1):132-137. Epub 2020 Aug 20.

Department of Dentistry, All India Institute of Medical Sciences, Jodhpur, Jodhpur, India.

Aim: The risk of excessive bleeding prompts physicians to discontinue aspirin in patients on low-dose, long-term therapy which in turn puts them at the risk from adverse cardiovascular and thrombotic events. Effect of low-dose aspirin therapy on platelet function was assessed using platelet aggregation method. The aim was to correlate the laboratory platelet function with cutaneous and clinical oral bleeding time (BT). Read More

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Evaluation of Fluorinated Cromolyn Derivatives as Potential Therapeutics for Alzheimer's Disease.

J Alzheimers Dis 2021 ;80(2):775-786

Genetics and Aging Research Unit, McCance Center for Brain Health, Mass General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA.

Background: Cromolyn is an anti-neuroinflammatory modulator with a multifactorial mechanism of action that has been shown to inhibit amyloid-β (Aβ) aggregation and enhance microglial uptake and clearance of Aβ.

Objective: We report the effects of fluoro-cromolyn derivatives on microglial cell toxicity and microglial clearance of Aβ42.

Methods: Microglial cell toxicity for cromolyn derivatives were determined in naive BV2 microglial cells. Read More

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January 2021

ALOX12 mutation in a family with dominantly inherited bleeding diathesis.

J Hum Genet 2021 Feb 10. Epub 2021 Feb 10.

Department of Pediatrics, Yamagata University School of Medicine, Yamagata, Japan.

The arachidonic acid (AA) cascade plays a significant role in platelet aggregation. AA released from membrane phospholipids is metabolized by cyclooxygenase (COX) pathway to thromboxane A (TXA) or by 12S-lipoxygenase (ALOX12) to 12-hydroperoxyeicosatetraenoic acid (12-HPETE). In contrast to a well-known role of the COX pathway in platelet aggregation, the role of ALOX12 is not well understood. Read More

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February 2021

In vitro antiplatelet activity of extract and its fractions of Paulownia Clone in Vitro 112 leaves.

Biomed Pharmacother 2021 May 6;137:111301. Epub 2021 Feb 6.

Department of Animal Nutrition, Poznań University of Life Sciences, Wołyńska 33, 60-637, Poznań, Poland. Electronic address:

Background: Paulownia Clone in Vitro 112, also known as Oxytree is a hybrid of Paulownia elongata and Paulownia fortunei, developed under laboratory conditions. Its seeds are sterile, making it a noninvasive variety that can only be propagated in the laboratory. In China, species from the Paulownia genus (Paulowniaceae) are widely used in traditional medicine for the treatment of infectious diseases, such as gonorrhea and erysipelas. Read More

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Disaggregated mortality from gastrointestinal cancers in Asian Americans: Analysis of United States death records.

Int J Cancer 2021 Jun 9;148(12):2954-2963. Epub 2021 Feb 9.

Stanford Center for Asian Health Research and Education (CARE), Stanford University School of Medicine, Stanford, California, USA.

Asian Americans (AAs) are heterogeneous, and aggregation of diverse AA populations in national reporting may mask high-risk groups. Gastrointestinal (GI) cancers constitute one-third of global cancer mortality, and an improved understanding of GI cancer mortality by disaggregated AA subgroups may inform future primary and secondary prevention strategies. Using national mortality records from the United States from 2003 to 2017, we report age-standardized mortality rates, standardized mortality ratios and annual percent change trends from GI cancers (esophageal, gastric, colorectal, liver and pancreatic) for the six largest AA subgroups (Asian Indians, Chinese, Filipinos, Japanese, Koreans and Vietnamese). Read More

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