162,635 results match your criteria Δnp63 activating

Omenn syndrome caused by a novel homozygous mutation in recombination activating gene 1.

Immunobiology 2021 Apr 28;226(3):152090. Epub 2021 Apr 28.

Laboratory of Genomics and Human Genetics,Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360 Casablanca, Morocco. Electronic address:

Omenn syndrome (OS) is a type of severe combined immunodeficiency (SCID) that is distinguished by, lymphadenopathy, hepatosplenomegaly, erythroderma, alopecia with normal to elevated T-cell counts, eosinophilia, and elevated serum IgE levels. Recombination activation gene (RAG) 1 or RAG2 mutations that result in partial V(D)J recombination activity are known to be the main cause of OS. Other genes (DCLRE1C, LIG4, IL7RA, common gamma chain, ADA, RMRP, and CHD7) have also been linked to OS, although with low frequency. Read More

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New indenopyrazole linked oxadiazole conjugates as anti-pancreatic cancer agents: Design, synthesis, in silico studies including 3D-QSAR analysis.

Bioorg Med Chem Lett 2021 May 5:128094. Epub 2021 May 5.

Organic Synthesis and Process Chemistry Division, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500007, India; Academy of Scientific and Innovative Research, Ghaziabad 201002, India; School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, New Delhi 110062, India. Electronic address:

To continue the quest of newer anticancer agents, herein a novel class of 1,4-Dihydroindenopyrazole linked oxadiazole conjugates 9(a-r) was designed, synthesized and experimented for their anti-proliferative activities against four different cancer cell lines (human) such as MDA MB-231 (breast), PANC-1 (pancreatic), MCF-7 (breast), and Caco-2 (Colorectal) by using MTT assay. Among the series compound 9h and 9m demonstrated significant potency against the PANC-1 (human pancreatic cancer cells) with IC value 7.4 μM and 4. Read More

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Ghrelin restores memory impairment following olfactory bulbectomy in mice by activating hippocampal NMDA1 and MAPK1 gene expression.

Behav Brain Res 2021 May 5:113341. Epub 2021 May 5.

Instituto de Fisiología Humana, Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET and Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, X5000ESU, Córdoba, Argentina; Functional Pharmacology, Department of Neuroscience, Uppsala University, BMC, Husargatan 3, Box 593, SE-751 24, Uppsala, Sweden.

Ghrelin (Ghrl) is an orexigenic peptide with potential roles in the modulation of anxiety- and depressive-like symptoms induced by bilateral olfactory bulbectomy (OB) in rodents. In the present work, we assessed whether intrahippocampal Ghrl could reverse OB-induced depressive-like and amnesic effects by regulating molecular mechanisms related to neuroplasticity. Adult female albino Swiss mice were divided into sham and OB groups, and infused with saline (S) or Ghrl 0. Read More

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Voluntary wheel-running exercise attenuates brain aging of rats through activating miR-130a-mediated autophagy.

Brain Res Bull 2021 May 5. Epub 2021 May 5.

Hubei Key Laboratory of Sport Training and Monitoring, College of Health Science, Wuhan Sports University, Wuhan, 430079, China. Electronic address:

Autophagy is a highly regulated intracellular process for the degradation of protein aggregates and damaged organelles. Recently, autophagy has been implicated in Alzheimer's disease (AD) and aging. Autophagy process is regulated by the recruitment and assembly of several autophagy-related genes (Atgs) such as, Atg7 and LC3, as the highly conserved and important markers involved in the regulation of autophagy. Read More

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Dual role of miR-1 in the development and function of sinoatrial cells.

J Mol Cell Cardiol 2021 May 5. Epub 2021 May 5.

Department of Biosciences, Università degli Studi di Milano, Milan, Italy. Electronic address:

miR-1, the most abundant miRNA in the heart, modulates expression of several transcription factors and ion channels. Conditions affecting the heart rate, such as endurance training and cardiac diseases, show a concomitant miR-1 up- or down-regulation. Here, we investigated the role of miR-1 overexpression in the development and function of sinoatrial (SAN) cells using murine embryonic stem cells (mESC). Read More

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StackIL6: a stacking ensemble model for improving the prediction of IL-6 inducing peptides.

Brief Bioinform 2021 May 8. Epub 2021 May 8.

Center of Data Mining and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.

The release of interleukin (IL)-6 is stimulated by antigenic peptides from pathogens as well as by immune cells for activating aggressive inflammation. IL-6 inducing peptides are derived from pathogens and can be used as diagnostic biomarkers for predicting various stages of disease severity as well as being used as IL-6 inhibitors for the suppression of aggressive multi-signaling immune responses. Thus, the accurate identification of IL-6 inducing peptides is of great importance for investigating their mechanism of action as well as for developing diagnostic and immunotherapeutic applications. Read More

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[Effects of RNA interference of CTHRC1 on proliferation and apoptosis of thyroid papillary cancer TCP-1 cells ].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Apr;41(4):549-554

Department of Oncology Surgery, General Hospital of Ningxia Medical University, Yinchuan 750004, China.

Objective: To explore the role of CTHRC1 in regulating the proliferation and apoptosis of papillary thyroid cancer cells.

Objective: Papillary thyroid cancer TPC-1 cells were transfected with a small interfering RNA (siRNA) targeting CTHRC1, with the cells transfected with a scrambled sequence as the negative control. The changes in cell proliferation and apoptosis were assessed using cell counting kit-8 (CCK-8) and flow cytometry with AV/PI double staining, respectively. Read More

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[Energy metabolism disorder and functional magnetic resonance imaging of the medial prefrontal cortex in mice with chronic unpredictable mild stress].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Apr;41(4):521-528

Medical Imaging Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To analyze spontaneous activities and energy metabolism in the medial prefrontal cortex (mPFC) of mice with chronic unpredictable mild stress (CUMS) and explore the correlation of these changes with the mTORC1 signaling pathway.

Objective: Normal C57Bl/6 mice were randomly divided into control group (=16) and depression model group (= 16), and the mice in the latter group were subjected to 8 weeks of modeling with CUMS. Behavioral tests including open field test, sucrose consumption test, tail suspension test and forced swimming test were performed, and the changes in prefrontal gray matter volume and the amplitude of low frequency fluctuation (ALFF) in the mice were detected with functional magnetic resonance imaging. Read More

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Caspase-3, a primary target for natural and synthetic compounds for cancer therapy.

Chem Biol Drug Des 2021 May 8. Epub 2021 May 8.

Pharmacy College Saifai, Uttar Pradesh University of Medical Sciences Saifai, Etawah, U.P., 206130, India.

Caspases, a group of protease enzymes (cysteine proteases) exist as inactive zymogens in the cells and execute apoptosis (programmed cell death). Caspase-3, an executioner caspase plays an imperative role in apoptosis and becomes a primary target for cancer treatment. A number of analogues of quinazoline, quinazolinone, indoloquinazolines, quinone, naphthoquinones, pyrroloiminoquinones, styrylquinolines, tetheredtetrahydroquinoline, fluoroquinolone, thiosemicarbazones, benzotriazole, pyrimidines, chalcone and carbazoles have been reported till date, representing caspase-3 mediated apoptosis for cancer therapy. Read More

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BDNF Participates in Chronic Constriction Injury-Induced Neuropathic Pain via Transcriptionally Activating P2X in Primary Sensory Neurons.

Mol Neurobiol 2021 May 7. Epub 2021 May 7.

Department of Anesthesiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China.

Neuropathic pain, resulting from the pathological changes of the somatosensory nervous system, remains a severe public health problem worldwide. The effect of treatment targeting neuropathic pain is very limited, as the underlying mechanism of neuropathic pain is largely unknown. In this study, we demonstrated that the expression level of brain-derived neurotrophic factor (BDNF) was remarkably and time-dependently increased in dorsal root ganglion (DRG) neurons. Read More

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Thyroid Hormone Deiodinases: Dynamic Switches in Developmental Transitions.

Endocrinology 2021 May 8. Epub 2021 May 8.

National Institute of Diabetes and Digestive and Kidney Diseases, Laboratory of Endocrinology and Receptor Biology, National Institutes of Health, Bethesda, Maryland.

Thyroid hormones exert pleiotropic, essential actions in mammalian, including human, development. These actions depend upon provision of thyroid hormones in the circulation but also to a remarkable extent upon deiodinase enzymes in target tissues that amplify or deplete the local concentration of the primary active form of the hormone T3 (3,5,3'-triiodothyronine), the high affinity ligand for thyroid hormone receptors. Genetic analyses in mice have revealed key roles for activating (DIO2) and inactivating (DIO3) deiodinases in cell differentiation fates and tissue maturation, ultimately promoting neonatal viability, growth, fertility, brain development and behavior, as well as metabolic, endocrine and sensory functions. Read More

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Knockout of immunotherapy prognostic marker genes eliminates the effect of the anti-PD-1 treatment.

NPJ Precis Oncol 2021 May 7;5(1):37. Epub 2021 May 7.

Department of Basic Medical Science, School of Medicine, Tsinghua University, Beijing, China.

The efficacy of immunotherapy is largely patient-specific due to heterogeneity in tumors. Combining statistic power from a variety of immunotherapies across cancer types, we found four biological pathways significantly correlated with patient survival following immunotherapy. The expression of immunotherapy prognostic marker genes (IPMGs) in these pathways can predict the patient survival with high accuracy not only in the TCGA cohort (89. Read More

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The integrin signaling network promotes axon regeneration via the Src-ephexin-RhoA GTPase signaling axis.

J Neurosci 2021 May 7. Epub 2021 May 7.

Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan

Axon regeneration is an evolutionarily conserved process essential for restoring the function of damaged neurons. In hermaphrodites, initiation of axon regeneration is regulated by the RhoA GTPase-ROCK (Rho-associated coiled-coil kinase)-regulatory non-muscle myosin light-chain phosphorylation signaling pathway. However, the upstream mechanism that activates the RhoA pathway remains unknown. Read More

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GCN4 regulates secondary metabolism through activating antioxidant genes expression under nitrogen limitation in .

Appl Environ Microbiol 2021 May 7. Epub 2021 May 7.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture; Microbiology Department, College of Life Sciences, Agricultural University, Jiangsu, Nanjing 210095, People's Republic of China.

Nitrogen limitation has been widely reported to affect the growth and development of fungi, and the transcription factor GCN4 (general control nonderepressible 4) is involved in nitrogen restriction. Here, we found that nitrogen limitation highly induced the expression of GCN4 and promoted the synthesis of ganoderic acid (GA), an important secondary metabolite in The activated GCN4 is involved in regulating GA biosynthesis. Besides, the accumulation of ROS is also affecting the synthesis of GA under nitrogen restrictions. Read More

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The potential endocrine disruption mechanism of anthelmintic drug niclosamide by activating estrogen receptors and estrogen-related receptors.

Toxicology 2021 May 4:152805. Epub 2021 May 4.

College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, PR China. Electronic address:

Niclosamide (NIC), a helminthic drug used widely for controlling schistosomiasis, can reportedly disrupt the endocrine system. However, its underlying mechanisms are still unclear. In this study, we revealed the potential endocrine disruption mechanism of NIC by activating estrogen receptors (ERs) and estrogen-related receptors (ERRs). Read More

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Time-dependent changes in striatal monoamine levels and gene expression following single and repeated amphetamine administration in rats.

Eur J Pharmacol 2021 May 4:174148. Epub 2021 May 4.

Instituto de Investigaciones Psicológicas; Centro de Investigación en Neurociencias, Universidad de Costa Rica. Electronic address:

As drug addiction may result from pathological usurpations of learning and memory's neural mechanisms, we focused on the amphetamine-induced time-dependent neurochemical changes associated with neural plasticity. We used juvenile rats as the risk for drug abuse is higher during adolescence. Experiment 1 served to define the appropriate amphetamine dose and the neurochemical effects of a single administration. Read More

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CD200R1 and CD200R1L expression is regulated during B cell development in swine and modulates the Ig production in response to the TLR7 ligand imiquimoid.

PLoS One 2021 7;16(5):e0251187. Epub 2021 May 7.

Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.

The CD200R family comprises a group of paired receptors that can modulate the activation of immune cells. They are expressed both on myeloid cells and lymphocyte subsets. Here we report that the expression of these receptors on porcine B cells is tightly regulated, being mainly expressed on mature cells. Read More

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The concept-sharing approach: a teaching strategy to promote objective-oriented learning and academic performance in medical students.

Adv Physiol Educ 2021 Jun;45(2):369-375

Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Traditionally teachers display the learning outline at the beginning and conclusions at the end of didactic teaching sessions, and students may find it difficult to understand how teaching activities relate to learning objectives and what they should study for assessments. We introduced the "concept-sharing approach" in our neurophysiology course. This approach explicates how the content relates with learning objectives throughout the entirety of each teaching session to help the students assimilate the learning material and direct their learning in an objective-orientated way. Read More

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The Role of the Core Attachment Positioning in Triggering Intramolecular Singlet Exciton Fission in Perylene Diimide Tetramers.

J Phys Chem B 2021 May 7. Epub 2021 May 7.

Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.

Previous studies have proposed that the presence of a flexible π-bridge linker is crucial in activating intramolecular singlet exciton fission (iSEF) in multichromophoric systems. In this study, we report the photophysical properties of three analogous perylene diimide (PDI) dendritic tetramers showing flexible/twisted π-bridged structures with α- and β-substitutions and a rigid/planar structure with a β-fused ring (βC) connection to a benzodithiophene-thiophene (BDT-Th) core. The rigidity and enhanced planarity of βC lead to significant intramolecular charge transfer and triplet formation via an intersystem crossing pathway. Read More

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The Emerging Role of B Cells in the Pathogenesis of NAFLD.

Hepatology 2021 May 7. Epub 2021 May 7.

Department of Integrative Biology & Physiology, University of Minnesota, United States.

Non-alcoholic fatty liver disease (NAFLD) is one of the leading causes of abnormal liver function worldwide. NAFLD refers to a group of liver conditions ranging from non-alcoholic fatty liver (NAFL) to non-alcoholic steatohepatitis (NASH), which involves inflammation, hepatocellular damage, and fibrosis. Triggering of inflammation in NASH is a key event in the progression of the disease and identifying the factors that initiate or dysregulate this process is needed to develop strategies for its prevention or treatment. Read More

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What do laboratory-forgetting paradigms tell us about use-inspired forgetting?

Cogn Res Princ Implic 2021 May 7;6(1):37. Epub 2021 May 7.

Department of Psychology, Vanderbilt University, Wilson Hall, 111 21st Ave S, Nashville, TN, 37212, USA.

Directed forgetting is a laboratory task in which subjects are told to remember some information and forget other information. In directed forgetting tasks, participants are able to exert intentional control over which information they retain in memory and which information they forget. Forgetting in this task appears to be mediated by intentional control of memory states in which executive control mechanisms suppress unwanted information. Read More

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Impact of Nutritional Epigenetics in Essential Hypertension: Targeting microRNAs in the Gut-Liver Axis.

Curr Hypertens Rep 2021 May 7;23(5):28. Epub 2021 May 7.

Microbiome Consortium, Center for Hypertension and Precision Medicine, Department of Physiology and Pharmacology, The University of Toledo College of Medicine and Life Sciences, Block Health Science Bldg, 3000 Arlington Ave, Toledo, OH, 43614, USA.

Purpose Of Review: To review the current knowledge on interactions between dietary factors and microRNAs (miRNAs) in essential hypertension (EH) pathogenesis.

Recent Findings: There exists an integration of maintenance signals generated by genetic, epigenetic, immune, and environmental (e.g. Read More

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Fructose-1,6-bisphosphatase aggravates oxidative stress-induced apoptosis in asthma by suppressing the Nrf2 pathway.

J Cell Mol Med 2021 May 7. Epub 2021 May 7.

Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, China.

Asthma is a chronic airway disease that causes excessive inflammation, oxidative stress, mucus production and bronchial epithelial cell apoptosis. Fructose-1,6-bisphosphatase (Fbp1) is one of the rate-limiting enzymes in gluconeogenesis and plays a critical role in several cancers. However, its role in inflammatory diseases, such as asthma, is unclear. Read More

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The Ca -binding protein sorcin stimulates transcriptional activity of the unfolded protein response mediator ATF6.

FEBS Lett 2021 May 7. Epub 2021 May 7.

Section of Cell Biology and Functional Genomics, Department of Metabolism, Digestion & Reproduction, Imperial College London, UK.

Sorcin is a calcium-binding protein involved in maintaining endoplasmic reticulum (ER) Ca stores. We have previously shown that overexpressing sorcin under the rat insulin promoter was protective against high fat diet-induced pancreatic beta cell dysfunction in vivo. Activating Transcription Factor 6 (ATF6) is a key mediator of the unfolded protein response (UPR) that provides cellular protection during the progression of ER stress. Read More

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Quantitative analysis of cell-free DNA by droplet digital PCR reveals the presence of EGFR mutations in non-malignant lung pathologies.

Monaldi Arch Chest Dis 2021 May 4. Epub 2021 May 4.

Division of Molecular Genetics and Cancer, Nitte University Centre For Science Education and Research (NUCSER), Nitte (Deemed to be University), Mangalore .

Tyrosine kinase inhibitors (TKIs) targeting epidermal growth factor receptor (EGFR) are effectively used in treatment of non-small cell lung cancer (NSCLC). Mutation profile of tyrosine kinase domain of EGFR determines the eligibility of the patients for tyrosine kinase inhibitor (TKI) therapy. Liquid biopsy, which relies on circulating tumor-derived nucleic acids, has emerged as an effective tool in lung cancer management with proven diagnostic, prognostic and predictive applications. Read More

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Cyclic AMP-dependent activation of ERK via GLP-1 receptor signalling requires the neuroendocrine cell-specific guanine nucleotide exchanger NCS-RapGEF2.

J Neuroendocrinol 2021 May 6:e12974. Epub 2021 May 6.

Section on Molecular Neuroscience, National Institute of Mental Health - Intramural Research Program, Bethesda, MD, USA.

Cyclic AMP activation of the Rap-Braf-MEK-ERK pathway after signalling initiated by the neuropeptide pituitary adenylate cyclase-activating peptide (PACAP), via the G -protein coupled receptor (G PCR) PAC1, occurs uniquely through the neuritogenic cAMP sensor Rap guanine nucleotide exchange factor 2 (NCS-RapGEF2) in Neuroscreen-1 (NS-1) neuroendocrine cells. We examined the expression of other Family B G PCRs in this cell line and assessed cAMP elevation and neuritogenesis after treatment with their cognate peptide ligands. Exposure of NS-1 cells to the VIPR1/2 agonist vasoactive intestinal polypeptide, or the GLP1R agonist exendin-4, did not induce neuritogenesis, or elevation of cAMP, presumably as a result of insufficient receptor protein expression. Read More

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POSTN Promotes the Proliferation of Spermatogonial Cells by Activating the Wnt/β-Catenin Signaling Pathway.

Reprod Sci 2021 May 6. Epub 2021 May 6.

Department of Stem Cells and Regenerative Medicine, Shenyang Key Laboratory of Stem Cell and Regenerative Medicine, China Medical University, Shenyang, 110013, Liaoning, China.

The self-renewal of spermatogonial cells (SCs) provides the foundation for life-long spermatogenesis. To date, only a few growth factors have been used for the culture of SCs in vitro, and how to enhance proliferation capacity of SCs in vitro needs further research. This study aimed to explore the effects of periostin (POSTN) on the proliferation of human SCs. Read More

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Influence of genetic variants in asparaginase pathway on the susceptibility to asparaginase-related toxicity and patients' outcome in childhood acute lymphoblastic leukemia.

Cancer Chemother Pharmacol 2021 May 7. Epub 2021 May 7.

Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.

Background: Asparaginase (ASNase) is a key component in the treatment protocols of childhood acute lymphoblastic leukemia (ALL). Asparagine synthetase (ASNS) and the basic region leucine zipper activating transcription factor 5 (ATF5) mediate the anti-leukemic effect of ASNase. Only a few reports studied the association between polymorphisms in these genes and treatment-related toxicity and response. Read More

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A Novel Modulator of STIM2-Dependent Store-Operated Ca2+ Channel Activity.

Acta Naturae 2021 Jan-Mar;13(1):140-146

Institute of Cytology of Russian Academy of Sciences, St. Petersburg, 194064 Russia.

Store-operated Ca entry is one of the main pathways of calcium influx into non-excitable cells, which entails the initiation of many intracellular processes. The endoplasmic reticulum Ca sensors STIM1 and STIM2 are the key components of store-operated Ca entry in mammalian cells. Under physiological conditions, STIM proteins are responsible for store-operated Ca entry activation. Read More

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Roxadustat and thyroid-stimulating hormone suppression.

Clin Kidney J 2021 May 20;14(5):1472-1474. Epub 2021 Jan 20.

Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan.

Hypoxia-inducible factor prolyl-hydroxylase inhibitors belong to a new class of orally administered drugs for treating anemia in patients with chronic kidney disease (CKD). The prevalence of hypothyroidism is disproportionately high in patients with CKD on hemodialysis. We report a rapid suppression of thyroid-stimulating hormone (TSH) and decrease in free triiodothyronine (T3) and free tetraiodothyronine levels after switching from darbepoetin alfa to roxadustat in a hemodialysis patient with hypothyroidism on levothyroxine therapy. Read More

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