65 results match your criteria Δnp63

IL-13 modulates ∆Np63 levels causing altered expression of barrier- and inflammation-related molecules in human keratinocytes: A possible explanation for chronicity of atopic dermatitis.

Immun Inflamm Dis 2021 Apr 1. Epub 2021 Apr 1.

Department of Pathology, School of Medicine, Sapporo Medical University, Sapporo, Japan.

Background: Barrier disruption and an excessive immune response in keratinocytes are now considered to have important roles in the pathophysiology of atopic dermatitis (AD). Furthermore, disturbed keratinocyte differentiation is considered to underlie AD. ΔNp63, a p53-like transcription factor, is a major regulator of keratinocyte differentiation. Read More

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Safety and efficacy of combination of suberoylamilide hydroxyamic acid and mitomycin C in reducing pro-fibrotic changes in human corneal epithelial cells.

Sci Rep 2021 Feb 23;11(1):4392. Epub 2021 Feb 23.

Stem Cell Research Lab, GROW Laboratory, Narayana Nethralaya Foundation, Narayana Nethralaya, Bangalore, Karnataka, India.

Corneal haze post refractive surgery is prevented by mitomycin c (MMC) treatment though it can lead to corneal endothelial damage, persistent epithelial defects and necrosis of cells. Suberanilohydroxamic acid (SAHA) however has been proposed to prevent corneal haze without any adverse effects. For clinical application we have investigated the short and long term outcome of cells exposed to SAHA. Read More

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February 2021

Pin1 and JNK1 cooperatively modulate TAp63γ.

FEBS Open Bio 2021 Mar 19;11(3):890-897. Epub 2021 Feb 19.

Center of Growth, Metabolism and Aging, Key Laboratory of Biological Resources and Ecological Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.

The p63 gene encodes at least 10 isoforms, which can be classified into TA and ∆N isotypes (TAp63 and ∆Np63 proteins) according to their differences at the N termini. TAp63γ is an important transcription factor. We previously reported that peptidyl-prolyl isomerase (PPI) Pin1 directly binds to TAp63γ protein and identified that serine 12 (S ) in the transactivation domain (TAD) of TAp63γ is required for regulation of its transcriptional activity. Read More

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Long-term palliation of lymph node oligometastatic ovarian carcinoma after repeated stereotactic body radiotherapy: case report.

Tumori 2020 Dec 10;106(6):NP63-NP66. Epub 2020 Sep 10.

Radiotherapy Oncology Centre, "Santa Maria" Hospital, Terni, Italy.

Introduction: Oligometastatic disease has emerged as an intermediate state between localized and systemic cancer. Improvements in diagnostic modalities such as functional imaging allow a greater frequency of oligometastases diagnosis. Patients with selected oligometastatic epithelial ovarian carcinoma (EOC) may be treated with metastasis-directed stereotactic body radiotherapy (SBRT) rather than chemotherapy. Read More

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December 2020

Maintaining protein stability of ∆Np63 via USP28 is required by squamous cancer cells.

EMBO Mol Med 2020 04 4;12(4):e11101. Epub 2020 Mar 4.

Department of Biochemistry and Molecular Biology, Protein Stability and Cancer Group, University of Würzburg, Würzburg, Germany.

The transcription factor ∆Np63 is a master regulator of epithelial cell identity and essential for the survival of squamous cell carcinoma (SCC) of lung, head and neck, oesophagus, cervix and skin. Here, we report that the deubiquitylase USP28 stabilizes ∆Np63 and maintains elevated ∆NP63 levels in SCC by counteracting its proteasome-mediated degradation. Impaired USP28 activity, either genetically or pharmacologically, abrogates the transcriptional identity and suppresses growth and survival of human SCC cells. Read More

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Sphere-forming corneal cells repopulate dystrophic keratoconic stroma: Implications for potential therapy.

World J Stem Cells 2020 Jan;12(1):35-54

Department of Ophthalmology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland 1023, New Zealand.

Background: Keratoconus is a degenerative corneal disease characterised by aberrant cell behaviour and loss of matrix that can result in vision loss. Cells extracted from peripheral corneas can form stem cell-enriched spheres, which have shown the potential to repopulate the normal peripheral corneal stroma upon sphere implantation but have not been previously studied in keratoconic tissue.

Aim: To investigate the therapeutic potential of stem cell-enriched spheres formed from extracted peripheral human corneal cells when introduced to keratoconic tissue. Read More

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January 2020

Piezoelectric Ultrasound Rhinoplasty.

Aesthet Surg J 2020 01;40(2):NP63-NP64

Professor of Plastic Surgery, Department of Plastic Surgery, Royal Free Hospital, Hampstead, London, UK.

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January 2020

p63 expression in Merkel cell carcinoma: comparative immunohistochemistry invokes TAp63 as the dominant isoform involved.

Hum Pathol 2020 03 22;97:60-67. Epub 2020 Jan 22.

Dalhousie University, Halifax, Nova Scotia, Canada B3H 1V8; Research Methods Unit, Nova Scotia Health Authority, Halifax, Nova Scotia, Canada B3H 1V7.

The literature suggests that p63 expression in Merkel cell carcinoma (MCC) is associated with a poor prognosis. p63 immunohistochemistry marks the 2 main isoforms of this transcriptional protein: TAp63 (tumor suppressor-like properties) and ∆Np63 (oncogenic properties). Little information about the isoform of relevance in MCC exists. Read More

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Circular RNA FAM114A2 suppresses progression of bladder cancer via regulating ∆NP63 by sponging miR-762.

Cell Death Dis 2020 01 22;11(1):47. Epub 2020 Jan 22.

Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.

Numerous evidences have shown that circular RNAs (circRNAs) play a key role in regulating the pathogenesis of cancer. However, the mechanism of circRNAs in urothelial carcinoma of bladder (UCB) remains largely unclear. In this study, we found circFAM114A2 was significantly downregulated both in UCB tissue specimens and cell lines, and the expression level was highly correlated with pathological TNM stage and grade. Read More

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January 2020

Inducible knockout of ∆Np63 alters cell polarity and metabolism during pubertal mammary gland development.

FEBS Lett 2020 03 17;594(6):973-985. Epub 2019 Dec 17.

Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.

The ∆Np63 isoform of the p53-family transcription factor Trp63 is a key regulator of mammary epithelial stem cells that is involved in breast cancer development. To investigate the role of ∆Np63 at different stages of normal mammary gland development, we generated a ∆Np63-inducible conditional knockout (cKO) mouse model. We demonstrate that the deletion of ∆Np63 at puberty results in depletion of mammary stem cell-enriched basal cells, reduces expression of E-cadherin and β-catenin, and leads to a closed ductal lumen. Read More

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Shp2-mediated MAPK pathway regulates ΔNp63 in epithelium to promote corneal innervation and homeostasis.

Lab Invest 2020 04 25;100(4):630-642. Epub 2019 Oct 25.

Indiana University School of Optometry, Bloomington, IN, USA.

Corneal nerve fibers serving sensory, reflex, and neurotrophic functions sustain corneal homeostasis and transparency to promote normal visual function. It is not known whether corneal epithelium is also important for the corneal innervation. Herein, we generated a compound transgenic mouse strain, K14rtTA;tetO-Cre (TC);Shp2, in which Shp2 was conditionally knocked out from K14-positive cells including corneal epithelium (Shp2) upon doxycycline (dox) administration. Read More

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∆Np63/p40 correlates with the location and phenotype of basal/mesenchymal cancer stem-like cells in human ER and HER2 breast cancers.

J Pathol Clin Res 2020 01 6;6(1):83-93. Epub 2019 Dec 6.

Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Brno, Czech Republic.

ΔNp63, also known as p40, regulates stemness of normal mammary gland epithelium and provides stem cell characteristics in basal and HER2-driven murine breast cancer models. Whilst ΔNp63/p40 is a characteristic feature of normal basal cells and basal-type triple-negative breast cancer, some receptor-positive breast cancers express ΔNp63/p40 and its overexpression imparts cancer stem cell-like properties in ER cell lines. However, the incidence of ER and HER2 tumours that express ΔNp63/p40 is unclear and the phenotype of ΔNp63/p40 cells in these tumours remains uncertain. Read More

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January 2020

A case of extensive ductal carcinoma in situ and sclerosing adenosis with metastasis on sentinel lymph node.

Tumori 2019 Dec 28;105(6):NP63-NP66. Epub 2019 Aug 28.

Department of General Surgery, Faculty of Medicine, Uludag University, Bursa, Turkey.

Introduction: Sclerosing adenosis is a form of adenosis characterized by lobulocentric architecture, glandular and stromal proliferation in which the stromal component compresses and distorts the glandular structures. Atypical epithelial proliferations such as atypical lobular hyperplasia, lobular carcinoma in situ, and ductal carcinoma in situ may accompany areas of sclerosing adenosis. We present a case of ductal carcinoma in situ and sclerosing adenosis with metastatic carcinoma on sentinel lymph node. Read More

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December 2019

The Effect of Calcium and Glucose Concentration on Corneal Epithelial Cell Lines Differentiation, Proliferation, and Focal Adhesion Expression.

Biores Open Access 2019 5;8(1):74-83. Epub 2019 Jun 5.

Trinity Centre for Biomedical Engineering, Trinity Biomedical Science Institute, Trinity College Dublin, University of Dublin, Dublin, Ireland.

It is known that culture media composition can affect cell behavior, morphology, and gene expression. However, in the case of corneal epithelial cells, the combined role of calcium and glucose concentration in media has not previously been examined. In this study, a human immortalized corneal epithelial cell line was used to examine the effect of glucose and calcium concentrations on these cells. Read More

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Oxidative Stress Causes Enhanced Secretion of YB-1 Protein that Restrains Proliferation of Receiving Cells.

Genes (Basel) 2018 Oct 22;9(10). Epub 2018 Oct 22.

Dipartimento di Biologia, Università degli Studi di Napoli, Federico II, 80126 Napoli, Italy.

The prototype cold-shock Y-box binding protein 1 (YB-1) is a multifunctional protein that regulates a variety of fundamental biological processes including cell proliferation and migration, DNA damage, matrix protein synthesis and chemotaxis. The plethora of functions assigned to YB-1 is strictly dependent on its subcellular localization. In resting cells, YB-1 localizes to cytoplasm where it is a component of messenger ribonucleoprotein particles. Read More

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October 2018

Characterization of distal airway stem-like cells expressing N-terminally truncated p63 and thyroid transcription factor-1 in the human lung.

Exp Cell Res 2018 11 27;372(2):141-149. Epub 2018 Sep 27.

Department of Molecular Medicine, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan. Electronic address:

Distal airway stem cells (DASCs) in the mouse lung can differentiate into bronchioles and alveoli. However, it remains unclear whether the same stem cells exist in the human lung. Here, we found that human lung epithelial (HuL) cells, derived from normal, peripheral lung tissue, in monolayer, mostly express both the N-terminally truncated isoform of p63 (∆Np63), a marker for airway basal cells, and thyroid transcription factor-1 (TTF-1), a marker for alveolar epithelial cells, even though these two molecules are usually expressed in a mutually exclusive way. Read More

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November 2018

ΔNp63 regulates the expression of hyaluronic acid-related genes in breast cancer cells.

Oncogenesis 2018 Aug 24;7(8):65. Epub 2018 Aug 24.

National Research Council of Italy, (CNR), Institute of Translational Pharmacology (IFT), Via Fosso del Cavaliere 100, Rome, 00133, Italy.

Triple negative breast cancers (TNBC) represent the most aggressive and clinically relevant breast carcinomas. On the basis of specific molecular signature, the majority of TNBC can be classified as basal-like breast carcinoma. Here, we report data showing that in basal-like breast carcinoma cells ΔNp63 is capable of sustaining the production of the hyaluronic acid (HA), one of the major component of the extracellular matrix (ECM). Read More

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Dependence receptor UNC5A restricts luminal to basal breast cancer plasticity and metastasis.

Breast Cancer Res 2018 05 2;20(1):35. Epub 2018 May 2.

Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.

Background: The majority of estrogen receptor-positive (ERα) breast cancers respond to endocrine therapies. However, resistance to endocrine therapies is common in 30% of cases, which may be due to altered ERα signaling and/or enhanced plasticity of cancer cells leading to breast cancer subtype conversion. The mechanisms leading to enhanced plasticity of ERα-positive cancer cells are unknown. Read More

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Inhibition of NF-B prevents the acidic bile-induced oncogenic mRNA phenotype, in human hypopharyngeal cells.

Oncotarget 2018 Jan 12;9(5):5876-5891. Epub 2017 Dec 12.

The Yale Larynx Laboratory, Department of Surgery, Yale School of Medicine, New Haven, CT, USA.

Bile-containing gastro-duodenal reflux has been clinically considered an independent risk factor in hypopharyngeal carcinogenesis. We recently showed that the chronic effect of acidic bile, at pH 4.0, selectively induces NF-B activation and accelerates the transcriptional levels of genes, linked to head and neck cancer, in normal hypopharyngeal epithelial cells. Read More

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January 2018

ΔNp63-mediated regulation of hyaluronic acid metabolism and signaling supports HNSCC tumorigenesis.

Proc Natl Acad Sci U S A 2017 12 21;114(50):13254-13259. Epub 2017 Nov 21.

Institute of Cell Biology and Neurobiology, National Research Council of Italy (CNR), 00015 Monterotondo (Rome), Italy;

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide, and several molecular pathways that underlie the molecular tumorigenesis of HNSCC have been identified. Among them, amplification or overexpression of ΔNp63 isoforms is observed in the majority of HNSCCs. Here, we unveiled a ΔNp63-dependent transcriptional program able to regulate the metabolism and the signaling of hyaluronic acid (HA), the major component of the extracellular matrix (ECM). Read More

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December 2017

A Case Report of Reconstruction of the Left Superior Vena Cava Using the Right Superior Vena Cava Autograft at Bilateral Bidirectional Superior Cavopulmonary Anastomosis.

World J Pediatr Congenit Heart Surg 2020 Jul 28;11(4):NP63-NP65. Epub 2017 Aug 28.

Division of Thoracic and Cardiovascular Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Niigata City, Japan.

The choice of graft material for reconstruction of the vena cava in pediatric patients remains controversial. We successfully treated an eight-month-old female patient with single ventricle physiology and long segment obstruction of the left superior vena cava using the right superior vena cava autograft at the time of bilateral bidirectional superior cavopulmonary anastomosis. Postoperative computed tomography confirmed the patency of the reconstruction. Read More

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Repression of p63 and induction of EMT by mutant Ras in mammary epithelial cells.

Proc Natl Acad Sci U S A 2016 10 28;113(41):E6107-E6116. Epub 2016 Sep 28.

Department of Biological Sciences, Columbia University, New York, NY 10027;

The p53-related transcription factor p63 is required for maintenance of epithelial cell differentiation. We found that activated forms of the Harvey Rat Sarcoma Virus GTPase (H-RAS) and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) oncogenes strongly repress expression of ∆Np63α, the predominant p63 isoform in basal mammary epithelial cells. This regulation occurs at the transcriptional level, and a short region of the ∆Np63 promoter is sufficient for repression induced by H-RasV12. Read More

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October 2016

Expression of p40 (∆Np63) protein in meningiomas, an unexpected finding: immunohistochemical study and evaluation of its possible prognostic role.

J Neurooncol 2016 09 9;129(3):405-413. Epub 2016 Jul 9.

Division of Neurosurgery, Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy.

According to the 2007 WHO (World Health Organization) Classification, meningiomas are divided into three grades of malignancy, with different recurrence rate, based exclusively on histopathological parameters. Loss/reduction of PgR (Progesterone Receptor) expression and increased Ki67 L.I. Read More

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September 2016

miR-21, miR-155, miR-192, and miR-375 Deregulations Related to NF-kappaB Activation in Gastroduodenal Fluid-Induced Early Preneoplastic Lesions of Laryngeal Mucosa In Vivo.

Neoplasia 2016 06 25;18(6):329-38. Epub 2016 May 25.

Department of Surgery, Yale Larynx Laboratory Yale School of Medicine, New Haven, CT, USA.

Unlabelled: Gastroduodenal refluxate found in the upper aerodigestive tract is not clinically uncommon. We recently demonstrated the neoplastic potential of gastroduodenal fluids (GDF) on hypopharyngeal mucosa, via NF-κB, using in vitro and in vivo models. Here we will explore the in vivo effect of GDF on laryngeal mucosa (LM) to induce early preneoplastic lesions related to NF-κB activation, along with deregulation of specific microRNA (miRNA) markers previously linked to laryngeal cancer. Read More

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p63 expression confers significantly better survival outcomes in high-risk diffuse large B-cell lymphoma and demonstrates p53-like and p53-independent tumor suppressor function.

Aging (Albany NY) 2016 Feb;8(2):345-65

Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

The role of p53 family member p63 in oncogenesis is the subject of controversy. Limited research has been done on the clinical implications of p63 expression in diffuse large B-cell lymphoma (DLBCL). In this study, we assessed p63 expression in de novo DLBCL samples (n=795) by immunohistochemistry with a pan-p63-monoclonal antibody and correlated it with other clinicopathologic factors and clinical outcomes. Read More

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February 2016

Immunocytochemical p63 expression discriminates between primary cutaneous follicle centre cell and diffuse large B cell lymphoma-leg type, and is of the TAp63 isoform.

Histopathology 2016 Jul 1;69(1):11-9. Epub 2016 Mar 1.

Dundee Cancer Centre, Ninewells Hospital and Medical School, Dundee, UK.

Aims: The p63 gene shares structural and functional homologies with the p53 family of transcriptional activators, but differs in exhibiting a consistent expression pattern in normal tissues. Although p63 is rarely mutated in malignancy studies of primary human tumours and cell lines suggest that p63 may promote tumour development. In non-Hodgkin's nodal lymphoma, TAp63 expression in follicular lymphoma (54%) and diffuse large B cell lymphoma (34%) has been described and correlated with the proliferative index. Read More

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