1,208 results match your criteria °c flow

Freezing chicken semen: Influence of base medium osmolality, cryoprotectants, cryoprotectant concentration, and cooling rate on post-thaw sperm survival.

Cryobiology 2022 Jun 28. Epub 2022 Jun 28.

Departamento de Reproducción Animal, INIA, 28040, Madrid, Spain.

A classical chicken semen diluent (Lake's 7.1 diluent) was modified to have lowered osmolalities (ranging from 290-410 mOsm/kg). The modified medium with physiological osmolality of 325 mOsm/kg allowed cold storage of fresh semen for several days with very little loss of membrane integrity and motility, while high osmolalities inhibited motility. Read More

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Tuning the Selectivity of the Hydrogenation/Hydrogenolysis of 5-Hydroxymethylfurfural under Batch Multiphase and Continuous-Flow Conditions.

ChemSusChem 2022 Jul 28;15(13):e202200503. Epub 2022 Jun 28.

Dipartimento di Scienze Molecolari e Nanosistemi, UniversitàCa' Foscari di Venezia, 30123, Venezia, Italy.

The hydrogenation/hydrogenolysis of 5-hydroxymethylfurfural (HMF) has been carried out either under single (aqueous) phase or batch multiphase (MP) conditions using mutually immiscible aqueous/hydrocarbon phases, 5 % Ru/C as a catalyst, and both with and without the use of trioctylmethyl phosphonium bis-(trifluoro methane) sulfonimide ([P ][NTf ]) as an ionic liquid (IL). Alternatively, the hydrogenation of HMF was explored in the continuous-flow (CF) mode with the same catalyst. By changing reaction parameters, experiments were optimized towards the formation of three products: 2,5-bis(hydroxy methyl)furan (BHMF), 2,5-bis(hydroxymethyl)tetrahydrofuran (BHMTHF), and 1-hydroxyhexane-2,5-dione (HHD), which were obtained in up to 92, 90, and 99 % selectivity, respectively, at quantitative conversion. Read More

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Development and Validation of an HPLC-UV Method for the Quantification of 4'-Hydroxydiclofenac Using Salicylic Acid: Future Applications for Measurement of In Vitro Drug-Drug Interaction in Rat Liver Microsomes.

Molecules 2022 Jun 2;27(11). Epub 2022 Jun 2.

School of Life Sciences, Pharmacy and Chemistry, Kingston University, Kingston upon Thames, London KT1 2EE, UK.

Salicylic acid is a key compound in nonsteroidal anti-inflammatory drugs that has been recently used for preventing the risk of hospitalization and death among COVID-19 patients and in preventing colorectal cancer (CRC) by suppressing two key proteins. Understanding drug-drug interaction pathways prevent the occurrence of adverse drug reactions in clinical trials. Drug-drug interactions can result in the variation of the pharmacodynamics and pharmacokinetic of the drug. Read More

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Over-toasting dehulled rapeseed meal and soybean meal, but not sunflower seed meal, increases prececal nitrogen and amino acid digesta flows in broilers.

Poult Sci 2022 Jul 8;101(7):101910. Epub 2022 Apr 8.

Animal Nutrition Group, Department of Animal Science, Wageningen University, The Netherlands. Electronic address:

Poorly digestible proteins may lead to increased protein fermentation in the ceca of broilers and hence, the production of potentially harmful metabolites. To evaluate effects of protein fermentation on gut health, an experimental contrast in ileal nitrogen (N) and amino acid (AA) flow is required. Therefore, our objective was to develop a model that creates a contrast in protein fermentation by increasing the prececal flow of protein within ingredients. Read More

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Culture and maintenance of neural progressive cells on cellulose acetate/graphene‑gold nanocomposites.

Int J Biol Macromol 2022 Jun 7;210:63-75. Epub 2022 May 7.

Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, P.O. Box 15875-1774, Tehran, Iran.

In this study, the first CA nanofibers were fabricated by electrospinning under optimal conditions: flow rate of 0.5 ml/h, a voltage of 20 kV, electrospinning distance of 15 cm, and an internal temperature of 25 °C, and humidity of 38%. The used Graphene/gold nanoparticles for CA performance improvement were examined by TGA, XRD, and SEM analysis. Read More

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Stability-indicating HPLC method development and validation for simultaneous estimation of metformin, dapagliflozin, and saxagliptin in bulk drug and pharmaceutical dosage form.

Biomed Chromatogr 2022 Jul 29;36(7):e5384. Epub 2022 Apr 29.

Department of Chemistry, JNTUH University College of Engineering Jagtial, Jagtial, India.

A simple, precise, and rapid stability-indicating reversed-phase-HPLC method was developed and validated for the estimation of metformin (MET), dapagliflozin (DAP), and saxagliptin (SAX) combination in bulk and tablet dosage forms. The proposed method uses a Kromasil C18 column (150 × 4.6 mm, 5 μm) with column oven temperature of 30°C and mobile phase containing a mixture of 60% phosphate buffer (pH = 3) and 40% acetonitrile. Read More

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Antioxidant, Cytotoxic, and Rheological Properties of Canola Oil Extract of (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania.

Plants (Basel) 2022 Mar 23;11(7). Epub 2022 Mar 23.

Department of Microbiology and Immunology, Faculty of Dental Medicine, Ovidius University of Constanta, 7 Ilarie Voronca Street, 900684 Constanta, Romania.

genus (, lichenized ) is a potent phytomedicine, due to phenolic secondary metabolites, with various pharmacological effects. Therefore, our study aimed to explore the antioxidant, cytotoxic, and rheological properties of (L.) Weber ex F. Read More

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A stability indicating RP-HPLC method for determination of the COVID-19 drug molnupiravir applied using nanoformulations in permeability studies.

J Pharm Biomed Anal 2022 May 26;214:114693. Epub 2022 Feb 26.

Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry, 06100 Sıhhiye, Ankara, Turkey. Electronic address:

Antiviral drugs have gained much more attention in recent years due to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and many drug candidates are currently under investigation in order to end pandemic. Molnupiravir, a prodrug of the synthetic nucleoside derivative N4-hydroxycytidine, is one of the promising candidates for SARS-CoV-2 treatment. In this study, a RP-HPLC method was developed for the determination of Molnupiravir and applied for in vitro permeability studies of self-emulsifying drug delivery system (SEDDS) formulations using Caco-2 cell line. Read More

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Separation of nordihydrocapsiate from capsiate and major capsaicinoid analogues using ultra high performance liquid chromatography.

Food Chem 2022 Jul 26;382:132585. Epub 2022 Feb 26.

Vegetable and Fruit Improvement Center, USDA National Center of Excellence, Department of Horticultural Sciences, Texas A&M University, 1500 Research Parkway, Suite A120, College Station, TX 77845, USA. Electronic address:

Accurate, rapid quantitation of the capsaicinoid and capsinoid compounds produced by peppers (Capsicum spp.) is essential to assess quality. Here, we developed a rapid ultra-high performance liquid chromatography method for the simultaneous separation of five major capsaicinoids and three major capsinoids from peppers. Read More

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Decontamination of 2-Chloroethyl ethyl sulfide on the surface by atmospheric pressure plasma jet.

J Hazard Mater 2022 02 19;424(Pt B):127536. Epub 2021 Oct 19.

State Key Laboratory of NBC Protection for Civilian, Beijing 102205, People's Republic of China. Electronic address:

Atmospheric pressure plasma jet (APPJ) were used to decontaminate the surface's 2-Chloroethyl ethyl sulfide (2-CEES), a kind of sulfur mustard (HD) simulant. The power of the APPJ device didn't exceed 7.77 W. Read More

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February 2022

Fabrication of chitin monoliths with controllable morphology by thermally induced phase separation of chemically modified chitin.

Carbohydr Polym 2022 Jan 20;275:118680. Epub 2021 Sep 20.

Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:

As a natural polymer, chitin has excellent biological properties such as biodegradability and immunological, antibacterial, and wound-healing activities and has numerous applications in cosmetics, drug delivery, and pharmaceuticals. Organic polymer monoliths have also drawn significant attention, owing to their high permeability, large surface area, and high mechanical strength. They are usually applied to separation, ion exchange, catalysis, and chromatography. Read More

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January 2022

Enantioselective separation of nonsteroidal anti-inflammatory drugs with amylose tris(3-chloro-5-methylphenylcarbamate) stationary phase in HPLC with a focus on enantiomeric quality control in six pharmaceutical formulations containing racemic mixtures or single stereoisomers.

Chirality 2021 12 15;33(12):938-950. Epub 2021 Oct 15.

School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.

In the present study, an accurate, rapid, and simple chiral HPLC-UV method with amylose tris(3-chloro-5-methylphenylcarbamate) as stationary phase was developed and applied for enantiomeric determination of six nonsteroidal anti-inflammatory drugs (NSAIDs) in the commercial pharmaceutical formulations, including (R,S)-ibuprofen, S-ibuprofen, (R,S)-ketoprofen, S-ketoprofen, S-naproxen, and (R,S)-loxoprofen sodium. Experiments on the influence of mobile phase composition, proportion of organic modifier, percentage of acid additives, and column temperature on enantioseparation were conducted to obtain the best separation condition. It was indicated that one mobile phase simply composed of acetonitrile-water (0. Read More

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December 2021

Strenuous exercise in warm environment is associated with improved microvascular function in sickle cell trait.

Eur J Appl Physiol 2022 Jan 11;122(1):185-197. Epub 2021 Oct 11.

Adaptation To Tropical Climate and Exercise Laboratory, EA3596, University of the French West Indies, Pointe-à-Pitre, Guadeloupe, France.

Purpose: Sickle cell trait is characterized by the presence of both normal and abnormal haemoglobin in red blood cells. The rate of exertional collapse is increased in athletes and military recruits who carry the trait, particularly in stressful environmental conditions. The aim of the present study was to investigate microvascular function and its determinants in response to intense exercise at control and warm environmental temperatures in carriers (AS) and non-carriers (AA) of sickle cell trait. Read More

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January 2022

A novel stability-indicating HPLC method for the determination of enantiomeric purity of eluxadoline drug: Amylose tris(3,5-dichlorophenyl carbamate) stationary phase.

Biomed Chromatogr 2022 Feb 2;36(2):e5260. Epub 2021 Nov 2.

Department of Organic Chemistry, AU College of Science and Technology, Visakhapatnam, India.

A simple and sensitive stability-indicating chiral HPLC method has been developed and validated per International Conference on Harmonization guidelines for the determination of enantiomeric purity of eluxadoline (Exdl). The impact of different mobile phase compositions and chiral stationary phases on the separation of Exdl enantiomer along with process- and degradation-related impurities has been studied. Homogeneity of Exdl and stable results of Exdl enantiomer in all degraded samples reveal the fact that the proposed method was specific (stability indicating). Read More

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February 2022

Effective Modified Xanthan Gum Fluid Loss Agent for High-Temperature Water-Based Drilling Fluid and the Filtration Control Mechanism.

ACS Omega 2021 Sep 7;6(37):23788-23801. Epub 2021 Sep 7.

School of Engineering and Technology, China University of Geosciences (Beijing), Beijing 100083, P.R. China.

Xanthan gum (XG) was widely used as an oilfield chemical treatment agent because of its environmental protection and diverse functions. With the increased drilling depth and formation complexity, the shortcomings such as poor solubility and low resistance to temperature were gradually exposed. In this study, a modified XG derivative XG--AAA was synthesized by grafting XG with acrylic acid (AA), acrylamide (AM), and 2-acrylamido-2-methylpropane sulfonic acid (AMPS). Read More

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September 2021

Human flavin-containing monooxygenase 1 and its long-sought hydroperoxyflavin intermediate.

Biochem Pharmacol 2021 11 10;193:114763. Epub 2021 Sep 10.

Department of Life Sciences and Systems Biology, University of Torino, Italy. Electronic address:

Out of the five isoforms of human flavin-containing monooxygenase (hFMO), FMO1 and FMO3 are the most relevant to Phase I drug metabolism. They are involved in the oxygenation of xenobiotics including drugs and pesticides using NADPH and FAD as cofactors. Majority of the characterization of these enzymes has involved hFMO3, where intermediates of its catalytic cycle have been described. Read More

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November 2021

Kynurenine Monooxygenase Expression and Activity in Human Astrocytomas.

Cells 2021 08 8;10(8). Epub 2021 Aug 8.

Neurochemistry and Behavior Laboratory, National Institute of Neurology and Neurosurgery "Manuel Velasco Suárez", Mexico City 14269, Mexico.

Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor. The enzyme indoleamine-2,3-dioxygenase (IDO), which participates in the rate-limiting step of tryptophan catabolism through the kynurenine pathway (KP), is associated with poor prognosis in patients with GBM. The metabolites produced after tryptophan oxidation have immunomodulatory properties that can support the immunosuppressor environment. Read More

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ET receptor-mediated vasodilation is regulated by estradiol in young women.

Am J Physiol Heart Circ Physiol 2021 09 20;321(3):H592-H598. Epub 2021 Aug 20.

Department of Kinesiology and Applied Physiology, University of Delaware, Newark, Delaware.

The endothelin-B (ET) receptor is a key regulator of vascular endothelial function in women. We have previously shown that the ET receptor mediates vasodilation in young women, an effect that is lost after menopause. However, the direct impact of changes in estradiol (E) on ET receptor function in women remains unclear. Read More

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September 2021

Heat-induced down-regulation of photosystem II protects photosystem I in honeysuckle (Lonicera japonica).

J Plant Res 2021 Nov 5;134(6):1311-1321. Epub 2021 Aug 5.

Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, The College of Forestry, Beijing Forestry University, Beijing, 100083, China.

Honeysuckle (Lonicera japonica Thunb.) is a traditional medicinal plant in China which is often threatened by high temperature at midday during summer. Heat-induced effects on the photosynthetic apparatus in honeysuckle are associated with a depression of the photosystem II (PSII) photochemical efficiency. Read More

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November 2021

Quantitative ecology associations between heterotrophic nitrification-aerobic denitrification, nitrogen-metabolism genes, and key bacteria in a tidal flow constructed wetland.

Bioresour Technol 2021 Oct 23;337:125449. Epub 2021 Jun 23.

College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.

This study explored the quantitative mechanisms of heterotrophic nitrification-aerobic denitrification (HN-AD) in a pilot-scale two-stage tidal flow constructed wetland (TFCW). The TFCW packed shale ceramsite (SC) and activated alumina (AA) at each stage, respectively, and aimed to improve decentralized wastewater treatment efficiency. In start-up phases, AA-TFCW accelerated NH-N decline, reaching transformation rates of 6. Read More

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October 2021

Eco-Friendly Chromatographic Methods for Determination of Acemetacin and Indomethacin; Greenness Profile Assessment.

J AOAC Int 2021 Dec;104(6):1485-1491

Cairo University, Faculty of Pharmacy, Analytical Chemistry Department, Kasr El-Aini Street, ET-11562 Cairo, Egypt.

Background: The green chemistry approach was developed for the purpose of saving the environment by using green solvents. Applying green analytical chemistry principles to traditional methods is considered a challenge. Acemetacin is a commonly used analgesic prodrug that bioactivates to indomethacin. Read More

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December 2021

Conformational and rheological properties of bacterial cellulose sulfate.

Int J Biol Macromol 2021 Jul 3;183:2326-2336. Epub 2021 Jun 3.

School of Stomatology, Lanzhou University, Lanzhou 730000, China.

In this study, a water-soluble bacterial cellulose sulfate (BCS) was prepared with sulfur trioxide pyridine complex (SO3· Py) in a lithium chloride (LiCl)/dimethylacetamide (DMAc) homogeneous solution system using bacterial cellulose (BC). The structural study showed that the value for the degrees of substitution of BCS was 1.23. Read More

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Supercritical fluid chromatography and liquid chromatography for isomeric separation of a multiple chiral centers analyte.

J Chromatogr A 2021 Aug 24;1651:462270. Epub 2021 May 24.

Univ. Lille, Inserm, Institut Pasteur de Lille, U1167 - Risk factors and molecular determinants of aging-related diseases, F-59000 Lille, France. Electronic address:

The development of a chiral separation strategy has always been a challenge of crucial importance, particularly in the pharmaceutical field. Chromatographic methods have become popular, particularly High Performance Liquid Chromatography and Supercritical Fluid Chromatography from a preparative scale point of view. A bioactive compound bearing three stereogenic centers was entrusted in our laboratory and the aim of this work was to obtain the complete resolution of the eight stereoisomers. Read More

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Mitochondria-targeted antioxidant MitoQ ameliorates ischaemia-reperfusion injury in kidney transplantation models.

Br J Surg 2021 09;108(9):1072-1081

Department of Surgery, University of Cambridge, Cambridge, UK.

Background: Ischaemia-reperfusion (IR) injury makes a major contribution to graft damage during kidney transplantation. Oxidative damage to mitochondria is an early event in IR injury. Therefore, the uptake, safety, and efficacy of the mitochondria-targeted antioxidant MitoQ were investigated in models of transplant IR injury. Read More

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September 2021

Alternative and Improved Stability-Indicating HPLC Method for the Assay of Eprinomectin and Determination of Its Related Compounds in Bulk Batches of Eprinomectin Drug Substance.

J AOAC Int 2021 Dec;104(6):1492-1504

Global Pharmaceutical Technical Support, Boehringer Ingelheim Animal Health USA Inc., 631 US Route 1 south, North Brunswick, NJ, 08902, USA.

Background: Eprinomectin is used as an active pharmaceutical ingredient (API) in various drug products by the animal health industry. Several major related impurities of eprinomectin API are not separated and coelute by the current United States Pharmacopeia (USP) method for eprinomectin.

Objective: The aim was to develop and validate a true and reliable stability-indicating reversed-phase (RP) HPLC method for assay and determination of related substances of eprinomectin in bulk batches of eprinomectin API. Read More

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December 2021

An efficient high-speed countercurrent chromatography method for preparative isolation of highly potent anti-cancer compound antroquinonol from Antrodia camphorata after experimental design optimized extraction.

J Sep Sci 2021 Jul 11;44(13):2655-2662. Epub 2021 May 11.

School of Pharmacy, Zhejiang University of Technology, Hangzhou, P. R. China.

To avoid irreversible stationary phase adsorption and tedious and time-consuming separation steps, high-speed countercurrent chromatography was employed for the preparative separation of anti-tumor compound antroquinonol from solid fermentation culture of Antrodia camphorata for the first time. A Box-Behnken experimental design, based on three parameters including liquid-to-solid ratio, extraction time, and extraction temperature, was applied to optimize the ultrasonic extraction procedure. The optimal extraction condition was set as follows: liquid-to-solid ratio: 49. Read More

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Simultaneous determination by UHPLC-PDA of major capsaicinoids and capsinoids contents in peppers.

Food Chem 2021 Sep 26;356:129688. Epub 2021 Mar 26.

Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, P.O. Box 40, 11510 Puerto Real, Cadiz, Spain.

Capsaicinoids and capsinoids compounds have been a focus of special attention for their health benefits. An effective and rapid Ultra-High-Performance Liquid Chromatography (UHPLC-PDA) method has been developed and validated for the simultaneous separation and quantitative determination of the major capsaicinoids and capsinoids present in peppers. The separation of all the compounds of interest was achieved in less than 2 min by means of an ACQUITY UPLC BEH rp-C18 column (100 mm × 2. Read More

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September 2021

Role of Bradykinin Type 2 Receptors in Human Sweat Secretion: Translational Evidence Does Not Support a Functional Relationship.

Skin Pharmacol Physiol 2021 1;34(3):162-166. Epub 2021 Apr 1.

Division of Biomedical Sciences, Marian University College of Osteopathic Medicine, Indianapolis, Indiana, USA.

Bradykinin increases skin blood flow via a cGMP mechanism but its role in sweating in vivo is unclear. There is a current need to translate cell culture and nonhuman paw pad studies into in vivo human preparations to test for therapeutic viability for disorders affecting sweat glands. Protocol 1: physiological sweating was induced in 10 healthy subjects via perfusing warm (46-48°C) water through a tube-lined suit while bradykinin type 2 receptor (B2R) antagonist (HOE-140; 40 μM) and only the vehicle (lactated Ringer's) were perfused intradermally via microdialysis. Read More

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November 2021

Saikosaponin-d improved the stemness of mouse neural stem cells and increased their thermotolerance potential.

Int J Dev Neurosci 2021 Jun 24;81(4):324-332. Epub 2021 Apr 24.

Department of Neonatology, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin, China.

Purpose: To investigate the effect of saikosaponin-d (Ssd) on proliferation, differentiation, and stemness of neural stem cells (NSCs), and to observe whether Ssd has a protective effect on NSCs at medium-high and high temperature.

Materials And Methods: NSCs were extracted from 15-day fetal mice. After subculture, Ssd treatment was performed. Read More

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Modification of activated carbon by MIL-53(Al) MOF to develop a composite framework adsorbent for CO capturing.

Environ Sci Pollut Res Int 2021 Jul 15;28(28):37929-37939. Epub 2021 Mar 15.

Department of Chemical Engineering, School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran.

In this research, a novel composite is synthesized based on activated carbon and MIL-53(Al) through the solution mixing method at different MOF weight fractions, and the CO loading of prepared samples are measured in the batch and continuous apparatus. The structure, crystallinity, surface area, and chemical functionality of activated carbon, MIL-53(Al), and developed composite are characterized through BET, X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). The CO and N adsorption capacity of activated carbon, MIL-53(Al), and composites are examined in an isothermal batch reactor at the pressure range 0-110 kPa and equilibrium temperature 305 K. Read More

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