Involvement of parental imprinting in the antisense regulation of onco-miR-372-373.

Authors:
Dr. Yonatan Stelzer, PhD
Dr. Yonatan Stelzer, PhD
Whitehead Institute, MIT
Postdoctoral Fellow
Cambridge, MA | United States
Ido Sagi
Ido Sagi
Silberman Institute of Life Sciences
Israel
Nissim Benvenisty
Nissim Benvenisty
The Hebrew University
Israel

Nat Commun 2013 ;4:2724

Stem Cell Unit, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.

The monoallelic nature of imprinted genes renders them highly susceptible to genetic and epigenetic perturbations, potentially resulting in transformation and disease. Here we show, using parthenogenetic induced pluripotent stem cells, an imprinted transcript that serves as an antisense regulator of onco-miR-372-3 (named anti-miR-371-3). As miR-372-3 have been shown to have an oncogenic role in testicular germ cell tumours, we study the involvement of their antisense transcript in these cells. Our results suggest that hypermethylation, leading to loss-of-expression of the imprinted antisense transcript, contributes to tumorigenic transformation by affecting the downstream target LATS2. Finally, we provide evidence for a tumour suppressive role of anti-miR-371-3, as its overexpression in tumour cells results in cell growth arrest and apoptosis, and prevents tumour formation on injection into immunodeficient mice.

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http://dx.doi.org/10.1038/ncomms3724DOI Listing
July 2014
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References

(Supplied by CrossRef)
Article in Oncol. Rep.
Nakanishi H. et al.
Oncol. Rep. 2004

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