Designing a novel mRNA vaccine against SARS-CoV-2

Overview

This study resorted to the emerging discipline of immunoinformatics in order to design a multi-epitope mRNA vaccine against the spike glycoprotein of SARS-CoV-2

Summary

It provides a detailed insight into the components that need to be present in an mRNA vaccine against SARS-CoV-2 in the face of COVID-19 pandemic.

Author Comments

ishtiaque ahammad
ishtiaque ahammad
National Institute of Biotechnology
Scientific Officer
Bioinformatics, Systems Biology
Dhaka | Bangladesh
This was one of the largest projects I undertook. My co-author Samia Sultana Lira helped enormously in elevating the quality of the research. This was also the first project where I enjoyed truly equal contribution from all authors.ishtiaque ahammad

Designing a novel mRNA vaccine against SARS-CoV-2: An immunoinformatics approach

International Journal of Biological Macromolecules

SARS-CoV-2 is the deadly virus behind COVID-19, the disease that went on to ravage the world and caused the biggest pandemic 21st century has witnessed so far. On the face of ongoing death and destruction, the urgent need for the discovery of a vaccine against the virus is paramount. This study resorted to the emerging discipline of immunoinformatics in order to design a multi-epitope mRNA vaccine against the spike glycoprotein of SARS-CoV-2. Various immunoinformatics tools were utilized to predict T and B lymphocyte epitopes. The epitopes were channeled through a filtering pipeline comprised of antigenicity, toxicity, allergenicity, and cytokine inducibility evaluation with the goal of selecting epitopes capable of generating both T and B cell-mediated immune responses. Molecular docking simulation between the epitopes and their corresponding MHC molecules was carried out. 13 epitopes, a highly immunogenic adjuvant, elements for proper sub-cellular trafficking, a secretion booster, and appropriate linkers were combined for constructing the vaccine. The vaccine was found to be antigenic, almost neutral at physiological pH, non-toxic, non-allergenic, capable of generating a robust immune response and had a decent worldwide population coverage. Based on these parameters, this design can be considered a promising choice for a vaccine against SARS-CoV-2.

June 2020
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Impact Factor: 5.160

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