Comparison of five solvents in the extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel

Overview

Background: Pomegranate peels are rich in bioactive compounds and could be an alternative natural source such as antioxidants. Aims: The aim of the present study was to assess the abilities of five solvents to extract phenolic antioxidants from pomegranate peels. Methods and Material: Pomegranate peels powder was subjected to extraction and the extraction yield was compared. The total phenolic, flavonoid, condensed and hydrolysable tannins contents were analyzed. Antioxidant activity of extracts was evaluated by two methods (DPPH* and ABTS•+ scavenging capacities) and results were then compared. Results: Results showed that the mixture methanol/water (50:50) allowed higher extraction yield (37.33±5.3%) than the others solvents (P<0.05). Further, the total phenolic, flavonoid and condensed tannins contents were highest in mixture water/methanol (50:50) extract. Phenolic antioxidants showed distinct reducing capacity and a high DPPH* inhibition values were recorded for all extracts with no significant differences (P>0.05) between ethanol and mixture water/methanol (50:50) extracts. All extracts exhibited high inhibition against ABTS•+ but with very large variation. Phenolic content and antioxidant activities were well positively correlated with each other. Conclusions: Our findings revealed that the choice of the extracting solvent affect considerably the extraction of phenolic antioxidants from pomegranate peels. ​

Comparison of five solvents in the extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel

The North African Journal of Food and Nutrition Research

Kennas A, Amellal-Chibane H. Comparison of five solvents in the extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel. Nor. Afr. J. Food Nutr. Res. January - June (2019); 03 (05): 140-147. https://doi.org/10.5281/zenodo.2567587

A B S T R A C T 

Background: Pomegranate peels are rich in bioactive compounds and could be an alternative natural source such as antioxidants. Aims: The aim of the present study was to assess the abilities of five solvents to extract phenolic antioxidants from pomegranate peels. Methods and Material: Pomegranate peels powder was subjected to extraction and the extraction yield was compared. The total phenolic, flavonoid, condensed and hydrolysable tannins contents were analyzed. Antioxidant activity of extracts was evaluated by two methods (DPPH* and ABTS•+ scavenging capacities) and results were then compared. Results: Results showed that the mixture methanol/water (50:50) allowed higher extraction yield (37.33±5.3%) than the others solvents (P<0.05). Further, the total phenolic, flavonoid and condensed tannins contents were highest in mixture water/methanol (50:50) extract. Phenolic antioxidants showed distinct reducing capacity and a high DPPH* inhibition values were recorded for all extracts with no significant differences (P>0.05) between ethanol and mixture water/methanol (50:50) extracts. All extracts exhibited high inhibition against ABTS•+ but with very large variation. Phenolic content and antioxidant activities were well positively correlated with each other. Conclusions:  Our findings revealed that the choice of the extracting solvent affect considerably the extraction of phenolic antioxidants from pomegranate peels.

Keywords: Pomegranate peels, Solvent extraction, Antioxidant activity, Phenolic antioxidants.

February 2019
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