Evaluation of Cytotoxicity of root canal irrigants on human red blood cells

Overview

The aim of this study was to analyze the cytotoxicity of various volumes of 5.25% of sodium hypochlorite, 2% of chlorhexidine gluconate and a mixture of a tetracycline isomer, an acid and a detergent (MTAD) by checking for hemolysis of human red blood corpuscles

Summary

This study suggests that the three irrigating solutions do cause detrimental effects on the diluted red blood corpuscles. A great deal of care should, therefore, be exercised when using 5.25% sodium hypochlorite during endodontic irrigation.

Author Comments

Dr Vijay Reddy Venumuddala, MDS
Dr Vijay Reddy Venumuddala, MDS
Smiline Dental Hospitals
Professor & Head
Conservative Dentistry and Endodontics
HYDERABAD, IN-TS | India
The clinical situation, concentration used, exposure time to the agent and the exposure surface area are important factors that affect the cytotoxicity of the irrigating solution. Therefore taking into account the findings of this study chlorhexidine has proven to have the least cytotoxic potential when compared to MTAD and sodium hypochlorite.Dr Vijay Reddy Venumuddala, MDS

Resources

Saudi Endod J [serial online] 2014 [cited 2020 Apr 19];4:1-6.
http://www.saudiendodj.com/text.asp?2014/4/1/1/127979

Comparative evaluation of the cytotoxicity of 5.25% sodium hypochlorite, 2% chlorhexidine and mixture of a tetracycline isomer, an acid and a detergent on human red blood corpuscles: An in-vitro study

Saudi Endodontic Journal

Saudi Endod J 2014;4:1-6

Aim: The aim of this study was to analyze the cytotoxicity of various volumes of 5.25% of sodium hypochlorite, 2% of chlorhexidine gluconate and mixture of a tetracycline isomer, an acid and a detergent (MTAD) by checking for hemolysis of human red blood corpuscles. Materials and Methods: A total volume of 100 μl of diluted red blood corpuscles obtained through centrifugation was added to three irrigating solutions (5.25% of sodium hypochlorite, 2% of chlorhexidine and MTAD) of six test tubes each. Individual irrigants per group were added in increasing volume starting from the second test tube keeping the first one as the control. After incubation for 3 min hemoglobin content was measured using an automated hemoanalyzer data was analyzed using one sample t-test. Results: Sodium hypochlorite is the most cytotoxic solution followed by MTAD and chlorhexidine. Conclusions: This study suggests that the three irrigating solutions do cause detrimental effects on the diluted red blood corpuscles. A great deal of care should therefore be exercised when using 5.25% sodium hypochlorite during endodontic irrigation.Keywords: 2% chlorhexidine, 5.25% sodium hypochlorite, cytotoxicity, diluted red blood corpuscles, irrigating solution, mixture of a tetracycline isomer, an acid and a detergent
February 2014
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