Star polymers to carry cargo into sick cells

Overview

Fixing cel functioning is important for human helth and quality of life. Specifically important is to develop carriers that will deliver macromolecules able to change the cel function into right path.

Summary

We have define the structure and important features of the star polymers that make tchem better carriers then other commonly used ones. The significant findings are: a) the size of the star polimer, 2) the specific cargo binding parts of the star, and 3) the ratio of amino to phosphate residues.

Functional star polymers as reagents for efficient nucleic acids delivery into HT-1080 cells.

International Journal of Polymeric Materials and Polymeric Biomaterials,

Int. J. Polym. Mater. Polym. Biomater. 2020 Jan. DOI: 10.1080/00914037.2020.1716227

Star polymers of N,N0-dimethylaminoethyl methacrylate (DMAEMA) and hydroxyl-bearing oligo(-ethylene glycol) methacrylate (OEGMA-OH) were used as pDNA and mRNA carriers. To the best of our knowledge, this is the first report of stars in the formation of polyplexes with mRNA. Polyplexes efficiently delivered nucleic acids into HT-1080 cells, while their cytotoxicity was kept significantly low due to the presence of OEGMA-OH units in the star. Hydroxyl functionalities were used for the introduction of fluorophore moieties for monitoring of the complex location after the cell uptake. Thus, versatile gene delivery vectors based upon functional star polymers were developed.
January 2020
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