Instantaneous three-dimensional sensing using spatial light modulator illumination with extended depth of field imaging.

Opt Express 2013 Jul;21(13):16007-21

Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, 550 West 122nd Street, New York, NY 10027, USA.

Imaging three-dimensional structures represents a major challenge for conventional microscopies. Here we describe a Spatial Light Modulator (SLM) microscope that can simultaneously address and image multiple targets in three dimensions. A wavefront coding element and computational image processing enables extended depth-of-field imaging. High-resolution, multi-site three-dimensional targeting and sensing is demonstrated in both transparent and scattering media over a depth range of 300-1,000 microns.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3971059PMC
http://dx.doi.org/10.1364/OE.21.016007DOI Listing
July 2013
12 Reads

Publication Analysis

Top Keywords

spatial light
8
light modulator
8
dimensions wavefront
4
three dimensions
4
wavefront coding
4
coding element
4
image processing
4
computational image
4
element computational
4
targets three
4
multiple targets
4
modulator slm
4
describe spatial
4
slm microscope
4
microscope simultaneously
4
image multiple
4
simultaneously address
4
processing enables
4
extended depth-of-field
4
scattering media
4

References

(Supplied by CrossRef)

Alivisatos et al.
Neuron 2012

Dowski et al.
Appl. Opt. 1995

Nikolenko et al.
Front Neural Circuits 2008

Voie et al.
J. Microsc. 1993

Huber et al.
J. Microsc. 2001

Huisken et al.
Science 2004

Holekamp et al.
Neuron 2008

Göbel et al.
Nat. Methods 2007

Grewe et al.
Nat. Methods 2010

Cheng et al.
Nat. Methods 2011

Katona et al.
Nat. Methods 2012

Similar Publications