Blood 2008 Feb 13;111(4):2347-53. Epub 2007 Dec 13.
Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research, and Curriculum in Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
MicroRNAs are regulated by gene alteration, transcription, and processing. Thus far, few studies have simultaneously assessed all 3 levels of regulation. Using real-time quantitative polymerase chain reaction (QPCR)-based arrays, we determined changes in gene copy number, pre-miRNA, and mature miRNA levels for the largest set of primary effusion lymphomas (PELs) to date. We detected PEL-specific miRNA gene amplifications, and concordant changes in pre-miRNA and mature miRNA. We identified 68 PEL-specific miRNAs. This defines the miRNA signature of PEL and shows that transcriptional regulation of pre-miRNA as well as mature miRNA levels contribute nonredundant information that can be used for the classification of human tumors.