Atherosclerotic plaque smooth muscle cells have a distinct phenotype.

Arterioscler Thromb Vasc Biol 2004 Jul 13;24(7):1283-9. Epub 2004 May 13.

Department of Pathology, University of Washington, Box 357335, Seattle, Wash 98195-7335, USA.

Objective: The present study addresses the question, "Are plaque smooth muscles cells (SMCs) genetically distinct from medial SMCs as reflected by the ability to maintain a distinctive expression phenotype in vitro?"

Methods And Results: Multiple cell strains were developed from carotid endarcterectomy specimens, and quadruplicate array hybridizations were completed for each sample. A new normalization protocol was developed and used to analyze the data. Permutation analysis suggests that most of the significant differences in expression could not have occurred by chance. A broad pattern of significant expression differences, consisting of almost 5% of the genes probed, was detected. Quantitative polymerase chain reaction (QPCR) confirmation was found in 70% of a subset of genes selected for validation.

Conclusions: The SMC cultures were nearly indistinguishable by morphological features, population doubling time, and sensitivity to cell death induced by Fas cross-linking. Surprisingly, array expression analysis identified differences so extensive that we conclude that plaque and medial SMCs are distinctly different SMC cell types.

Download full-text PDF

Source
http://dx.doi.org/10.1161/01.ATV.0000132401.12275.0cDOI Listing
July 2004
3 Reads

Publication Analysis

Top Keywords

medial smcs
8
plaque smooth
8
genes selected
4
specimens quadruplicate
4
quadruplicate array
4
array hybridizations
4
selected validationconclusions
4
subset genes
4
confirmation 70%
4
completed sample
4
70% subset
4
hybridizations completed
4
validationconclusions smc
4
endarcterectomy specimens
4
morphological features
4
cell strains
4
features population
4
multiple cell
4
strains developed
4
indistinguishable morphological
4

Altmetric Statistics

References

(Supplied by CrossRef)

Am J Pathol 1998

Similar Publications