Role of NADH/NADPH oxidase-derived H2O2 in angiotensin II-induced vascular hypertrophy.

Hypertension 1998 Sep;32(3):488-95

From the Department of Medicine, Division of Cardiology, Emory University, Atlanta, GA 30322, USA.

Recent evidence suggests that oxidative mechanisms may be involved in vascular smooth muscle cell (VSMC) hypertrophy. We previously showed that angiotensin II (Ang II) increases superoxide production by activating an NADH/NADPH oxidase, which contributes to hypertrophy. In this study, we determined whether Ang II stimulation of this oxidase results in H2O2 production by studying the effects of Ang II on intracellular H2O2 generation, intracellular superoxide dismutase and catalase activity, and hypertrophy. Ang II (100 nmol/L) significantly increased intracellular H2O2 levels at 4 hours. Neither superoxide dismutase activity nor catalase activity was affected by Ang II; the SOD present in VSMCs is sufficient to metabolize Ang II-stimulated superoxide to H2O2, which accumulates more rapidly than it is degraded by catalase. This increase in H2O2 was inhibited by extracellular catalase, diphenylene iodonium, an inhibitor of the NADH/NADPH oxidase, and the AT1 receptor blocker losartan. In VSMCs stably transfected with antisense p22phox, a critical component of the NADH/NADPH oxidase in which oxidase activity was markedly reduced, Ang II-induced production of H2O2 was almost completely inhibited, confirming that the source of Ang II-induced H2O2 was the NADH/NADPH oxidase. Using a novel cell line that stably overexpresses catalase, we showed that this increased H2O2 is a critical step in VSMC hypertrophy, a hallmark of many vascular diseases. Inhibition of intracellular superoxide dismutase by diethylthiocarbamate (1 mmol/L) also resulted in attenuation of Ang II-induced hypertrophy (62+/-2% inhibition). These data indicate that AT1 receptor-mediated production of superoxide generated by the NADH/NADPH oxidase is followed by an increase in intracellular H2O2, suggesting a specific role for these oxygen species and scavenging systems in modifying the intracellular redox state in vascular growth.

Download full-text PDF

Source
http://dx.doi.org/10.1161/01.hyp.32.3.488DOI Listing
September 1998
2 Reads

Publication Analysis

Top Keywords

nadh/nadph oxidase
20
ang ii-induced
12
superoxide dismutase
12
intracellular h2o2
12
h2o2
10
ang
9
catalase activity
8
vsmc hypertrophy
8
intracellular superoxide
8
oxidase
7
hypertrophy
6
superoxide
6
intracellular
6
catalase
5
nadh/nadph
5
oxidase oxidase
4
p22phox critical
4
component nadh/nadph
4
critical component
4
markedly reduced
4

References

(Supplied by CrossRef)

Oncogene 1993

Similar Publications