Serial analysis of gene expression during elongation of the peri-implantation porcine trophectoderm (conceptus).

Physiol Genomics 2005 Jan 9;20(2):188-94. Epub 2004 Nov 9.

Biotechnology and Germplasm Laboratory, United States Department of Agriculture Agricultural Research Service, Beltsville, Maryland 20705, USA.

Conceptus loss during the preimplantation and early postimplantation period hinders the efficiency of swine reproduction. Significant conceptus loss occurs during trophectoderm elongation between gestational day 11 (D11) and day 12 (D12). Elongation of the porcine conceptus is a key stage of development during which maternal recognition of pregnancy, initial placental development, and preparation for implantation occurs. The objective of this study was to establish comparative transcriptome profiles of D11 ovoid and D12 filamentous conceptuses and thereby identify temporally regulated genes essential for developmental progression during conceptus elongation. Serial analysis of gene expression (SAGE) libraries were constructed from in vivo derived ovoid and filamentous swine conceptuses to yield a total of 42,389 tags (ovoid) and 42,391 tags (filamentous) representing 14,464 and 13,098 putative unique transcripts, respectively. Statistical analysis of tag frequencies revealed the differential expression of 431 tags between libraries (P < 0.05). Nucleotide sequence alignment searches on public databases provided SAGE tag annotation and gene ontology assignments. Comparisons between the SAGE profiles of ovoid and filamentous conceptuses revealed increased expression of key genes in the steroidogenesis [cytochrome P-450(scc) (CYP11A1), aromatase (CYP19A), and steroidogenic acute regulatory protein (STAR)] and oxidative stress response pathways [microsomal glutathione S-transferase 1 (MGST1) and copper-zinc superoxide dismutase (SOD1)]. Differential expression of these genes in the steroidogenic and oxidative stress response pathways was confirmed by real-time PCR. These results validate the utility of SAGE in the pig and establish an initial model linking gene expression profiles at the pathway level with phenotypic progression from ovoid to filamentous stages of conceptus development.

Download full-text PDF

Source
http://dx.doi.org/10.1152/physiolgenomics.00157.2004DOI Listing
January 2005
36 Reads

Publication Analysis

Top Keywords

gene expression
12
ovoid filamentous
12
oxidative stress
8
filamentous conceptuses
8
conceptus loss
8
stress response
8
response pathways
8
analysis gene
8
serial analysis
8
differential expression
8
conceptus
6
expression
6
ovoid
5
filamentous
5
libraries 005
4
005 nucleotide
4
tags libraries
4
431 tags
4
revealed differential
4
expression 431
4

References

(Supplied by CrossRef)

Similar Publications