Differential gene expression in colon cancer of the caecum versus the sigmoid and rectosigmoid.

Gut 2005 Mar;54(3):374-84

Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital/Skejby, Brendstrupgaardsvej 100, DK- 8200 Aarhus N, Denmark.

Background And Aims: There are epidemiological, morphological, and molecular differences between normal mucosa as well as between adenocarcinomas of the right and left side of the large bowel. The aim of this study was to investigate differences in gene expression.

Methods: Oligonucleotide microarrays (GeneChip) were used to compare gene expression in 45 single samples from normal mucosa and sporadic colorectal carcinomas (Dukes' B and C) of the caecum compared with the sigmoid and rectosigmoid. Findings were validated by real time polymerase chain reaction.

Results: Fifty eight genes were found to be differentially expressed between the normal mucosa of the caecum and the sigmoid and rectosigmoid (p<0.01), including pS2, S100P, and a sialyltransferase, all being expressed at higher levels in the caecum. A total of 118 and 186 genes were differentially expressed between normal and right or left sided tumours of the colon, showing more pronounced differences in Dukes' C than B tumours. Thirty genes differentially expressed in tumour tissue were common to adenocarcinomas of both sides, including known tumour markers such as the matrix metalloproteinases. Keratins 8, 19, and 20 as well as carbonic anhydrases (II, IV, VII) showed side specific expression and were downregulated in left sided tumours whereas teratocarcinoma growth factor and cyclooxygenase 2 (COX-2) were upregulated in left sided adenocarcinomas. Immunohistochemical analysis confirmed differences in side specific expression for cytokeratin 20 and COX-2.

Conclusions: Differences in gene expression between normal mucosa as well as between adenocarcinomas of the caecum and sigmoid or rectosigmoid exist and should be taken into account when examining new targeted therapeutic regimens.

Download full-text PDF

Source
http://dx.doi.org/10.1136/gut.2003.036848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1774427PMC
March 2005
1 Read

Publication Analysis

Top Keywords

sigmoid rectosigmoid
12
normal mucosa
12
gene expression
8
large bowel
4
expression single
4
compare gene
4
single samples
4
samples normal
4
sporadic colorectal
4
mucosa sporadic
4
genechip compare
4
oligonucleotide microarrays
4
investigate differences
4
study investigate
4
aim study
4
gene expressionmethods
4
expressionmethods oligonucleotide
4
bowel aim
4
colorectal carcinomas
4
microarrays genechip
4

Altmetric Statistics

Similar Publications