Engineering cell shape and function.

Science 1994 Apr;264(5159):696-8

Biotechnology Processing Engineering Center, Massachusetts Institute of Technology, Cambridge 02139.

An elastomeric stamp, containing defined features on the micrometer scale, was used to imprint gold surfaces with specific patterns of self-assembled monolayers of alkanethiols and, thereby, to create islands of defined shape and size that support extracellular matrix protein adsorption and cell attachment. Through this technique, it was possible to place cells in predetermined locations and arrays, separated by defined distances, and to dictate their shape. Limiting the degree of cell extension provided control over cell growth and protein secretion. This method is experimentally simple and highly adaptable. It should be useful for applications in biotechnology that require analysis of individual cells cultured at high density or repeated access to cells placed in specified locations.

Download full-text PDF

Source
http://dx.doi.org/10.1126/science.8171320DOI Listing
April 1994

Publication Analysis

Top Keywords

cell extension
4
matrix protein
4
extracellular matrix
4
protein adsorption
4
adsorption cell
4
cell attachment
4
adaptable applications
4
applications biotechnology
4
support extracellular
4
defined shape
4
shape limiting
4
create islands
4
shape size
4
biotechnology require
4
growth protein
4
size support
4
secretion method
4
attachment technique
4
experimentally simple
4
separated defined
4

Similar Publications