Presence of a potent transcription activating sequence in the p53 protein.

Authors:
S Fields S K Jang

Science 1990 Aug;249(4972):1046-9

Department of Microbiology, School of Medicine, State University of New York, Stony Brook 11794.

The p53 gene is frequently mutated in a wide variety of human cancers. However, the role of the wild-type p53 gene in growth control is not known. Hybrid proteins that contain the DNA binding domain of yeast GAL4 and portions of p53 have been used to show that the p53 protein contains a transcription-activating sequence that functions in both yeast and mammalian cells. The NH2-terminal 73 residues of p53 activated transcription in mammalian cells as efficiently as the herpes virus protein VP16, which contains one of the strongest known activation domains. Combined with previous data that showed p53 is localized to the nucleus and can bind to DNA, these results support the idea that one function of p53 is to activate the transcription of genes that suppress cell proliferation.

Download full-text PDF

Source
http://dx.doi.org/10.1126/science.2144363DOI Listing
August 1990

Publication Analysis

Top Keywords

mammalian cells
8
p53 gene
8
p53
8
p53 protein
8
transcription mammalian
4
activated transcription
4
p53 activated
4
residues p53
4
cells efficiently
4
efficiently herpes
4
protein vp16
4
virus protein
4
herpes virus
4
yeast gal4
4
nh2-terminal residues
4
sequence functions
4
transcription-activating sequence
4
protein transcription-activating
4
portions p53
4
yeast mammalian
4

Altmetric Statistics

Similar Publications