Genome editing. The new frontier of genome engineering with CRISPR-Cas9.

Science 2014 Nov;346(6213):1258096

Department of Regulation in Infection Biology, Helmholtz Centre for Infection Research, D-38124 Braunschweig, Germany. Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, Department of Molecular Biology, Umeå University, S-90187 Umeå, Sweden. Hannover Medical School, D-30625 Hannover, Germany.

The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism, which has set the stage for remarkable developments using this technology to modify, regulate, or mark genomic loci in a wide variety of cells and organisms from all three domains of life. These results highlight a new era in which genomic manipulation is no longer a bottleneck to experiments, paving the way toward fundamental discoveries in biology, with applications in all branches of biotechnology, as well as strategies for human therapeutics.

Download full-text PDF

Source
http://www.sciencemag.org/cgi/doi/10.1126/science.1258096
Publisher Site
http://dx.doi.org/10.1126/science.1258096DOI Listing
November 2014
209 Reads

Publication Analysis

Top Keywords

genome engineering
8
mark genomic
4
regulate mark
4
modify regulate
4
technology modify
4
genomic loci
4
cells organisms
4
variety cells
4
wide variety
4
loci wide
4
developments technology
4
set stage
4
discovery elucidation
4
initial discovery
4
biology initial
4
repeat biology
4
elucidation crispr-cas9
4
crispr-cas9 enzyme
4
organisms three
4
mechanism set
4

References

(Supplied by CrossRef)
Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae
Rudin et al.
Genetics 1989
Site-specific recombination determined by I-SceI, a mitochondrial group I intron-encoded endonuclease expressed in the yeast nucleus
Plessis et al.
Genetics 1992

Similar Publications