The chemical genomic portrait of yeast: uncovering a phenotype for all genes.

Science 2008 Apr;320(5874):362-5

Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA.

Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ( approximately 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition.

Download full-text PDF

Source
http://dx.doi.org/10.1126/science.1150021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794835PMC
April 2008
14 Reads

Publication Analysis

Top Keywords

chemical genomic
8
phenotypes nonessential
4
uncover phenotypes
4
97% gene
4
conditions 97%
4
nonessential fraction
4
genome performed
4
fraction genome
4
functions uncover
4
gene deletions
4
deletion strain
4
consequence rich
4
exhibited measurable
4
deletions exhibited
4
medium difficult
4
stress conditions
4
difficult study
4
study functions
4
1144 chemical
4
strain presence
4

References

(Supplied by CrossRef)

Schreiber et al.
2005

Winzeler et al.
Science 1999

Giaever et al.
Nature; Physical Science (London) 2002

PNAS 2004

Gu et al.
Nature; Physical Science (London) 2003

Deutscher et al.
Nature genetics 2006

Papp et al.
Nature; Physical Science (London) 2004

Genetics 2005

PNAS 2006

Kellis et al.
Nature; Physical Science (London) 2004

Parsons et al.
Nature biotechnology 2004

Similar Publications