Evolutionary relationship of phototrophic bacteria in the alpha-Proteobacteria based on farnesyl diphosphate synthase.

Microbiology 2002 Jun;148(Pt 6):1923-1929

The International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita-shi, Osaka 565-0871, Japan1.

Partial sequences of farnesyl diphosphate (FPP) synthase genes derived from the Rhodobacter-Rhodovulum group and from the Rhodopseudomonas palustris-Bradyrhizobium japonicum group of the alpha-Proteobacteria were subjected to phylogenetic analysis to investigate the relationships of phototrophic and non-phototrophic bacteria in the alpha-Proteobacteria . The four Rhodovulum species formed a monophyletic group within the Rhodobacter cluster, and Agrobacterium ferrugineum IAM 12616(T) intermingled with the Rhodobacter species. This topology is in good agreement with the 16S rRNA phylogeny, although the FPP synthase gene was more divergent than the 16S rRNA. On the other hand, strains of the phototrophic Rps. palustris formed a cluster far from that of the non-phototrophic Bradyrhizobium japonicum strains. Moreover, Rps. palustris strains were differentiated from the nodule-forming B. japonicum, Mezorhizobium loti MAFF 303099 and Sinorhizobium sp. NGR 234 in the FPP synthase phylogeny. This relationship does not agree with the 16S rRNA phylogeny, wherein Rps. palustris was more closely related to B. japonicum than to strains of the Rhodobacter-Rhodovulum group. These results suggest that the FPP synthase gene of Rps. palustris diverged from that of B. japonicum.

Download full-text PDF

Source
http://dx.doi.org/10.1099/00221287-148-6-1923DOI Listing
June 2002
2 Reads

Publication Analysis

Top Keywords

fpp synthase
16
rps palustris
16
16s rrna
12
bacteria alpha-proteobacteria
8
japonicum strains
8
farnesyl diphosphate
8
rhodobacter-rhodovulum group
8
rrna phylogeny
8
synthase gene
8
japonicum
5
synthase
5
rhodobacter species
4
sinorhizobium ngr
4
topology good
4
agreement 16s
4
good agreement
4
303099 sinorhizobium
4
intermingled rhodobacter
4
species topology
4
ngr 234
4

Altmetric Statistics

References

(Supplied by CrossRef)

Adachi et al.
1996
Farnesyl diphosphate synthetase: molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiae
Anderson et al.
J Biol Chem 1989

Ausubel et al.
1995
Farnesyl diphosphate synthase gene of three phototrophic bacteria and its use as a phylogenetic marker
Cantera et al.
Int J Syst Evol Microbiol 2002
Bacteriochlorophyll-containing Rhizobium species
Fleischman et al.
Adv Photosynth 1995
Multiple sequence alignment with Clustal X
Jeanmougin et al.
Trends Biochem Sci 1998

Kawasaki et al.
1997

Similar Publications