Synthetic biology, systems biology, and metabolic engineering of Yarrowia lipolytica toward a sustainable biorefinery platform.

J Ind Microbiol Biotechnol 2020 Jul 4. Epub 2020 Jul 4.

Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, Baltimore, MD, 21250, USA.

Yarrowia lipolytica is an oleaginous yeast that has been substantially engineered for production of oleochemicals and drop-in transportation fuels. The unique acetyl-CoA/malonyl-CoA supply mode along with the versatile carbon-utilization pathways makes this yeast a superior host to upgrade low-value carbons into high-value secondary metabolites and fatty acid-based chemicals. The expanded synthetic biology toolkits enabled us to explore a large portfolio of specialized metabolism beyond fatty acids and lipid-based chemicals. In this review, we will summarize the recent advances in genetic, omics, and computational tool development that enables us to streamline the genetic or genomic modification for Y. lipolytica. We will also summarize various metabolic engineering strategies to harness the endogenous acetyl-CoA/malonyl-CoA/HMG-CoA pathway for production of complex oleochemicals, polyols, terpenes, polyketides, and commodity chemicals. We envision that Y. lipolytica will be an excellent microbial chassis to expand nature's biosynthetic capacity to produce plant secondary metabolites, industrially relevant oleochemicals, agrochemicals, commodity, and specialty chemicals and empower us to build a sustainable biorefinery platform that contributes to the prosperity of a bio-based economy in the future.

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http://dx.doi.org/10.1007/s10295-020-02290-8DOI Listing
July 2020

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References

(Supplied by CrossRef)

LT Cordova et al.
Curr Opin Chem Biol 2016

J Levering et al.
Curr Opin Biotechnol 2015

J Quarterman et al.
Appl Microbiol Biotechnol 2017

M Groenewald et al.
Crit Rev Microbiol 2014

M Kavscek et al.
Bmc Syst Biol 2015

SB Shi et al.
Front Microbiol 2017

R Ledesma-Amaro et al.
Trends Biotechnol 2016

S Michely et al.
PLoS One 2013

H Devillers et al.
Microbiol Resour Announc 2016

H Devillers et al.
Microbiol Resour Announc 2019

F Darvishi et al.
Appl Microbiol Biotechnol 2018

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