Evaluation of inflammatory markers interleukin-6 (IL-6) and matrix metalloproteinase-9 (MMP-9) in asthma.

J Asthma 2017 Aug 26;54(6):584-593. Epub 2016 Oct 26.

c Center of Excellence in Molecular Biology and Regenerative Medicine (CEMR), Department of Biochemistry, J.S.S Medical College, JSS University , Mysuru , Karnataka , India.

Introduction: Even though IL-6 and MMP-9 are associated with airway inflammation in asthma, there is paucity of data in Indian population.

Objective: To determine the levels of IL-6 and MMP-9 in the serum of patients suffering from asthma, and correlate with (a) disease severity, as per GINA guidelines; (b) clinical phenotypes; and (c) response to treatment.

Methodology: The levels of IL-6 and MMP-9 were compared between moderate persistent asthma (n = 25), severe persistent asthma (n = 25) and normal controls (n = 30). IL-6 and MMP-9 were measured by ELISA (R&D Systems Inc., USA and Canada) and compared between controls and asthmatics and between groups of different asthma severity, clinical variables, spirometry, and allergen sensitization. Spirometry was repeated after 2 months of ICS+LABA to assess response to treatment in relation to baseline IL-6 and MMP-9 levels.

Results: We observed a significant difference in both IL-6 and MMP-9 levels among asthmatics versus controls (p < 0.001), moderate versus severe persistent asthma (p < 0.001). A significant negative correlation was observed between MMP-9 and pre-bronchodilator FEV and FVC, but not with IL-6. There was no association between IL-6 and MMP-9 with asthma duration, total IgE, AEC, number of allergens sensitized and degree of sensitization. No significant correlation (p > 0.5) was observed with IL-6 and MMP-9 levels and FEV improvement after 2 months of ICS+LABA.

Conclusion: Higher levels of IL-6 and MMP-9 were observed in asthmatics as compared to controls and in severe persistent asthma as compared to moderate persistent asthma, higher levels of MMP-9 was associated with lower lung functions.

Download full-text PDF

Source
http://dx.doi.org/10.1080/02770903.2016.1244828DOI Listing
August 2017
16 Reads

Publication Analysis

Top Keywords

il-6 mmp-9
36
persistent asthma
20
severe persistent
12
mmp-9
12
levels il-6
12
il-6
11
asthma
10
mmp-9 asthma
8
compared controls
8
moderate persistent
8
mmp-9 associated
8
mmp-9 levels
8
compared moderate
8
correlation observed
8
higher levels
8
levels
6
persistent
5
assess response
4
treatment relation
4
response treatment
4

References

(Supplied by CrossRef)

Doshi V et al.
Ind J Pharmacol 2002

Similar Publications