A multidrug resistance transporter from human MCF-7 breast cancer cells.

Proc Natl Acad Sci U S A 1998 Dec;95(26):15665-70

Greenebaum Cancer Center of the University of Maryland, Baltimore MD 21201, USA.

MCF-7/AdrVp is a multidrug-resistant human breast cancer subline that displays an ATP-dependent reduction in the intracellular accumulation of anthracycline anticancer drugs in the absence of overexpression of known multidrug resistance transporters such as P glycoprotein or the multidrug resistance protein. RNA fingerprinting led to the identification of a 2.4-kb mRNA that is overexpressed in MCF-7/AdrVp cells relative to parental MCF-7 cells. The mRNA encodes a 655-aa [corrected] member of the ATP-binding cassette superfamily of transporters that we term breast cancer resistance protein (BCRP). Enforced expression of the full-length BCRP cDNA in MCF-7 breast cancer cells confers resistance to mitoxantrone, doxorubicin, and daunorubicin, reduces daunorubicin accumulation and retention, and causes an ATP-dependent enhancement of the efflux of rhodamine 123 in the cloned transfected cells. BCRP is a xenobiotic transporter that appears to play a major role in the multidrug resistance phenotype of MCF-7/AdrVp human breast cancer cells.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC28101PMC
http://dx.doi.org/10.1073/pnas.95.26.15665DOI Listing
December 1998

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References

(Supplied by CrossRef)

Kessel et al.
Cancer Research 1968

Biedler et al.
Cancer Research 1970

Ling et al.
Journal of cellular physiology 1974

Gros et al.
Nature; Physical Science (London) 1986

Cole et al.
Science 1992

Chen et al.
Journal of Biological Chemistry 1990

Lee et al.
Journal of cellular biochemistry 1997

Doyle et al.
British Journal of Cancer 1995

Liang et al.
Science 1992

Liang et al.
Cancer Research 1992

Ross et al.
Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K 1996

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