Identification of ERF-1 as a member of the AP2 transcription factor family.

Proc Natl Acad Sci U S A 1997 Apr;94(9):4342-7

Department of Surgery, Stanford University, Stanford, CA 94305, USA.

The ERF-1 transcription factor was previously shown to be involved in the regulation of estrogen receptor (ER) gene transcription in hormonally responsive breast and endometrial carcinomas. In this study we sought to identify the gene for ERF-1. ERF-1 activates ER gene transcription by binding to the imperfect palindrome CCCTGCGGGG within the promoter of the ER gene. ERF-1 protein was purified from the ER-positive breast carcinoma cell line, MCF7, utilizing ion exchange and DNA affinity chromatography. Peptide sequence analysis was used to isolate a 2.7 kb cDNA clone from an MCF7 cDNA library. This cDNA encodes a protein of 48 kDa previously identified as the AP2gamma transcription factor. By gel-shift analysis, in vitro synthesized ERF-1 comigrates with MCF7 native ERF-1 complex and demonstrates identical sequence binding specificity as native ERF-1. In addition, AP2 polyclonal antisera supershifts both in vitro synthesized and native ERF-1 complexes. These results show that ERF-1 is a member of the AP2 family of developmentally regulated transcription factors. Given the central role of ER expression in breast carcinoma biology, ERF-1 is likely to regulate expression of a set of genes characteristic of the hormonally-responsive breast cancer phenotype.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC20724PMC
http://dx.doi.org/10.1073/pnas.94.9.4342DOI Listing
April 1997

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References

(Supplied by CrossRef)

Knight et al.
Cancer Research 1977

Sigurdsson et al.
New England Journal of Medicine 1990

Ricketts et al.
Cancer Research 1991

Henry et al.
British Journal of Cancer 1988

Fuqua et al.
Journal of cellular biochemistry 1993

Weigel et al.
Cancer Research 1993

Ottaviano et al.
Cancer Research 1994

Lapidus et al.
Clinical Cancer Research 1996

deConinck et al.
Molecular and Cellular Biology 1995

Cicatiello et al.
Molecular Endocrinology 1995

Nucleic Acids Research 1983

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