An integrated microfluidic device for rapid serodiagnosis of amebiasis.

Biomicrofluidics 2013 21;7(1):11101. Epub 2013 Feb 21.

Department of Environmental Science and Engineering, Fudan University, 220 Handan Road, Shanghai 200433, People's Republic of China.

A microfluidic device was successfully fabricated for the rapid serodiagnosis of amebiasis. A micro bead-based immunoassay was fabricated within integrated microfluidic chip to detect the antibody to Entamoeba histolytica in serum samples. In this assay, a recombinant fragment of C terminus of intermediate subunit of galactose and N-acetyl-D-galactosamine-inhibitable lectin of Entamoeba histolytica (C-Igl, aa 603-1088) has been utilized instead of the crude antigen. This device was validated with serum samples from patients with amebiasis and showed great sensitivity. The serodiagnosis can be completed within 20 min with 2 μl sample consumption. The device can be applied for the rapid and cheap diagnosis of other infectious disease, especially for the developing countries with very limited medical facilities.

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http://dx.doi.org/10.1063/1.4793222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592878PMC
January 2014
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