Intracellular imaging of HeLa cells by non-functionalized NaYF4 : Er3+, Yb3+ upconverting nanoparticles.

Nanoscale 2010 Apr 17;2(4):495-8. Epub 2009 Nov 17.

Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke St. W., Montreal, QC H4B 1R6, Canada.

We report on the efficient incorporation of non-functionalized NaYF(4) : Er(3+), Yb(3+) nanoparticles inside HeLa live cancer cells by direct endocytosis. The efficient two-photon excited near-infrared-to-visible upconversion fluorescence of these nanoparticles is then used to obtain high-contrast intracellular fluorescence images of single cells. These images reveal a redistribution of the nanoparticles inside the cell as the incubation time increases. Thus, non-functionalized NaYF(4) : Er(3+), Yb(3+) nanoparticles emerge as very promising fluorescence probes for real-time imaging of cellular dynamics.

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http://dx.doi.org/10.1039/b9nr00236gDOI Listing
April 2010
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