Genetically encoded optical indicators for the analysis of neuronal circuits.

Authors:
Thomas Knöpfel

Nat Rev Neurosci 2012 Oct 30;13(10):687-700. Epub 2012 Aug 30.

RIKEN Brain Science Institute, 2-1 Hirosawa, Wako City, Saitama 351-0198, Japan.

In a departure from previous top-down or bottom-up strategies used to understand neuronal circuits, many forward-looking research programs now place the circuit itself at their centre. This has led to an emphasis on the dissection and elucidation of neuronal circuit elements and mechanisms, and on studies that ask how these circuits generate behavioural outputs. This movement towards circuit-centric strategies is progressing rapidly as a result of technological advances that combine genetic manipulation with light-based methods. The core tools of these new approaches are genetically encoded optical indicators and actuators that enable non-destructive interrogation and manipulation of neuronal circuits in behaving animals with cellular-level precision. This Review examines genetically encoded reporters of neuronal function and assesses their value for circuit-oriented neuroscientific investigations.

Download full-text PDF

Source
http://dx.doi.org/10.1038/nrn3293DOI Listing
October 2012
2 Reads

Publication Analysis

Top Keywords

genetically encoded
12
neuronal circuits
12
encoded optical
8
optical indicators
8
neuronal
5
animals cellular-level
4
behavioural outputs
4
outputs movement
4
generate behavioural
4
circuit-centric strategies
4
behaving animals
4
strategies progressing
4
cellular-level precision
4
movement circuit-centric
4
studies circuits
4
elements mechanisms
4
circuit elements
4
indicators actuators
4
precision review
4
mechanisms studies
4

References

(Supplied by CrossRef)

CM Gray et al.
Nature 1989

PJ Uhlhaas et al.
Trends Cogn. Sci. 2010

M Tsodyks et al.
Science 1999

A Grinvald et al.
Biopolymers 2003

A Arieli et al.
J. Neurophysiol. 1995

G Buzsáki et al.
2006

KR Delaney et al.
Proc. Natl Acad. Sci. USA 1994

CD Harvey et al.
Nature 2012

Y Ikegaya et al.
Science 2004

WE Skaggs et al.
Science 1996

D Shoham et al.
Neuron 1999

Similar Publications