Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing.

Nat Methods 2011 Feb 9;8(2):139-42. Epub 2011 Jan 9.

Department of Physics and Astronomy, University of California Los Angeles, Los Angeles, California, USA.

In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.

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http://www.nature.com/articles/nmeth.1552
Publisher Site
http://dx.doi.org/10.1038/nmeth.1552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076599PMC
February 2011
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