Fundamentals of experimental design for cDNA microarrays.

Authors:
Gary A Churchill

Nat Genet 2002 Dec;32 Suppl:490-5

The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.

Microarray technology is now widely available and is being applied to address increasingly complex scientific questions. Consequently, there is a greater demand for statistical assessment of the conclusions drawn from microarray experiments. This review discusses fundamental issues of how to design an experiment to ensure that the resulting data are amenable to statistical analysis. The discussion focuses on two-color spotted cDNA microarrays, but many of the same issues apply to single-color gene-expression assays as well.

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http://dx.doi.org/10.1038/ng1031DOI Listing
December 2002
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