Somatic histone H3 alterations in pediatric diffuse intrinsic pontine gliomas and non-brainstem glioblastomas.

Nat Genet 2012 Jan 29;44(3):251-3. Epub 2012 Jan 29.

Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

To identify somatic mutations in pediatric diffuse intrinsic pontine glioma (DIPG), we performed whole-genome sequencing of DNA from seven DIPGs and matched germline tissue and targeted sequencing of an additional 43 DIPGs and 36 non-brainstem pediatric glioblastomas (non-BS-PGs). We found that 78% of DIPGs and 22% of non-BS-PGs contained a mutation in H3F3A, encoding histone H3.3, or in the related HIST1H3B, encoding histone H3.1, that caused a p.Lys27Met amino acid substitution in each protein. An additional 14% of non-BS-PGs had somatic mutations in H3F3A causing a p.Gly34Arg alteration.

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http://www.nature.com/articles/ng.1102
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http://dx.doi.org/10.1038/ng.1102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3288377PMC
January 2012
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