Simultaneous Multi-plane Imaging of Neural Circuits.

Neuron 2016 Jan 7;89(2):269-84. Epub 2016 Jan 7.

Neurotechnology Center, Department of Biological Sciences, Columbia University, New York, NY 10027, USA.

Recording the activity of large populations of neurons is an important step toward understanding the emergent function of neural circuits. Here we present a simple holographic method to simultaneously perform two-photon calcium imaging of neuronal populations across multiple areas and layers of mouse cortex in vivo. We use prior knowledge of neuronal locations, activity sparsity, and a constrained nonnegative matrix factorization algorithm to extract signals from neurons imaged simultaneously and located in different focal planes or fields of view. Our laser multiplexing approach is simple and fast, and could be used as a general method to image the activity of neural circuits in three dimensions across multiple areas in the brain.

Download full-text PDF

Source
http://www.cell.com/neuron/pdf/S0896-6273(15)01085-5.pdf
Web Search
http://linkinghub.elsevier.com/retrieve/pii/S089662731501085
Publisher Site
http://dx.doi.org/10.1016/j.neuron.2015.12.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4724224PMC
January 2016
28 Reads

Publication Analysis

Top Keywords

neural circuits
12
multiple areas
8
nonnegative matrix
4
constrained nonnegative
4
sparsity constrained
4
activity sparsity
4
factorization algorithm
4
signals neurons
4
extract signals
4
algorithm extract
4
locations activity
4
matrix factorization
4
knowledge neuronal
4
areas layers
4
populations multiple
4
neuronal populations
4
layers mouse
4
mouse cortex
4
neurons imaged
4
prior knowledge
4

Altmetric Statistics

Similar Publications