A high-throughput in vitro model of human embryo attachment.

Fertil Steril 2012 Apr 15;97(4):974-8. Epub 2012 Feb 15.

Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia.

Objective: To develop a simple, high-throughput and widely applicable in vitro human implantation model that assesses trophoblast spheroid attachment to human uterine epithelial cells.

Design: Experimental study to establish and validate the model.

Setting: Cell culture. CELL(S): Lines BeWo, RL95-2, and AN3-CA.

Intervention(s): Labeling trophoblast spheroids with green fluorescence, selecting spheroids of size similar to implanting blastocysts by use of cell strainers, and assessing spheroid attachment by use of an automated microplate reader.

Main Outcome Measure(s): Establishment of a simple, reliable, and high-throughput in vitro model to study human embryo implantation.

Result(s): The assay enabled rapid fluorometric assessment of spheroid attachment to human endometrial epithelial cells (RL95-2 and AN3-CA) under different experimental conditions. The high-throughput assay was confirmed by conventional counting method to be highly reproducible and accurate.

Conclusion(s): The described methodology provides for the first time a high-throughput assay for the study of human embryo implantation and a promising tool for screening potential inhibitors or enhancers of embryo attachment.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fertnstert.2012.01.116DOI Listing
April 2012
5 Reads

Publication Analysis

Top Keywords

spheroid attachment
12
human embryo
12
embryo attachment
8
study human
8
attachment human
8
high-throughput vitro
8
high-throughput assay
8
vitro model
8
human
6
high-throughput
5
attachment
5
cell strainers
4
strainers assessing
4
accurateconclusions described
4
assessing spheroid
4
automated microplate
4
microplate readermain
4
blastocysts cell
4
highly reproducible
4
reproducible accurateconclusions
4

Similar Publications