Monoclonal antibody specific for IDH1 R132H mutation.

Acta Neuropathol 2009 Nov 2;118(5):599-601. Epub 2009 Oct 2.

Department of Neuropathology, Institute of Pathology, Ruprecht-Karls-University Heidelberg, Im Neuenheimer Feld 220/221, 69120 Heidelberg, Germany.

IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. We developed a mouse monoclonal antibody targeting the IDH1 R132H mutation. Here, we show the high specificity and sensitivity of this antibody on Western blots and tissue sections from formalin fixed paraffin embedded tumor specimens. This antibody is highly useful for tumor classification, in detecting single infiltrating tumor cells and for the characterization of the cellular role of mutant IDH1 protein.

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http://dx.doi.org/10.1007/s00401-009-0595-zDOI Listing
November 2009
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