The CpG-rich promoter of human LDH-C is differentially methylated in expressing and nonexpressing tissues.

Authors:
C Bonny E Goldberg

Dev Genet 1995 ;16(2):210-7

Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois, USA.

A comparison of nucleotide sequences of murine Ldh-a and Ldh-c genes and human LDH-A, LDH-B, and LDH-C reveals that mouse Ldh-c has lost the CpG "island" present in the genes for the somatic isozymes. However, the human LDH-C gene has a CpG-rich region of 230 bp surrounding its promoter. Endonuclease sensitivity coupled with polymerase chain reaction (PCR) demonstrate the presence of nine heavily methylated sites in this region in different somatic cells. The same sites are specifically hypomethylated in expressing tissues. 3' sites bordering the CpG-rich region appear to be methylated in both expressing and nonexpressing tissues. Furthermore, the methylated promoter forms a specific complex in vitro with a methyl-DNA binding protein. Evolutionary and functional implications of these observations are discussed.

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http://dx.doi.org/10.1002/dvg.1020160213DOI Listing
June 1995

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References

(Supplied by CrossRef)

Alcivar et al.
Biol Reprod 1991

Ariel et al.
Proc Natl Acad Sci USA 1991

Battistuzzi et al.
Proc Natl Acad Sci USA 1985

Bird et al.
Nucleic Acids Res 1980

Bird et al.
Nature 1986

Bird et al.
Cell 1985

Boyes et al.
Cell 1991

Boyes et al.
EMBO J 1992

Chung et al.
Biochem J 1985

Coulondre et al.
Nature 1978

Devereux et al.
Nucleic Acids Res 1984

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