Preparation of Cell Cultures and Vaccinia Virus Stocks.

Curr Protoc Microbiol 2015 Nov 3;39:14A.3.1-14A.3.18. Epub 2015 Nov 3.

National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland.

The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included.

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http://doi.wiley.com/10.1002/9780471729259.mc14a03s39
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081219PMC
http://dx.doi.org/10.1002/9780471729259.mc14a03s39DOI Listing
November 2015
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References

(Supplied by CrossRef)
Gene expression using the vaccinia virus/T7 RNA polymerase hybrid system
Elroy‚ÄźStein O. et al.
Curr. Protoc. Mol. Biol. 2001

Linscott W.D. et al.
1998

Moss B. et al.
2013

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