Trypan Blue Exclusion Test of Cell Viability.

Authors:
Warren Strober

Curr Protoc Immunol 2015 Nov 2;111:A3.B.1-A3.B.3. Epub 2015 Nov 2.

National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland.

The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells).

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Source
http://dx.doi.org/10.1002/0471142735.ima03bs111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6716531PMC
November 2015
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