Br J Urol 1998 Dec;82(6):882-7
Division of Basic and Pharmaceutical Sciences, Albany College of Pharmacy, NY, USA.
Neurourol Urodyn 2000 ;19(6):701-12
Division of Basic and Pharmaceutical Sciences, Albany College of Pharmacy, Albany, New York 12208, USA.
Partial outlet obstruction results in marked metabolic as well as contractile alterations. Specifically, the ratio of anaerobic to oxidative metabolism is significantly greater in hypertrophied than normal bladder smooth muscle, lactate dehydrogenase (LDH) and lactic acid production are increased, and the contractile apparatus is altered to allow for metabolically more efficient tension generation. In addition, contractile responses of hypertrophied bladder are apparently more resistant than those of normal bladder to hypoxia. Read More
Pharmacology 1998 Sep;57(3):139-47
Division of Basic and Pharmaceutical Sciences, Albany College of Pharmacy, Albany, N.Y., USA.
Purpose: We studied the effects of hypoxia followed by reoxygenation and an in vitro model of ischemia (hypoxia + substrate [glucose] deprivation) followed by reperfusion (reoxygenation + substrate replacement) on the contractile response of rabbit urinary bladder strips to nonrepetitive and repetitive field stimulation (FS), and correlated the results with the rate of lipid peroxidation. We view repetitive FS as a model for hyperreflexia.
Methods: The effects of repetitive and nonrepetitive FS on the contractile responses of isolated strips of rabbit bladder to FS, carbachol, and KCl were determined in the presence of 3 different incubation media: O2 + glucose (normal physiological medium); N2 + glucose (in vitro hypoxia), and N2 - glucose (in vitro ischemia). Read More
Neurourol Urodyn 1999 ;18(2):129-37
Division of Basic and Pharmaceutical Sciences, Albany College of Pharmacy, Stratton VA Medical Center, New York 12208, USA.
The effect of repetitive stimulation, in the presence and absence of diltiazem or pinacidil, on the contractile responses of isolated strips of rabbit bladder detrusor to field stimulation and carbachol, after 2 hr of incubation in a medium that serves as an in vitro model of ischemia (oxygen and substrate depleted Tyrode's solution), was determined. Our results are summarized as follows: a) The magnitude of the contractile dysfunctions after in vitro ischemia was enhanced by repetitive stimulation. b) Pre-incubation of isolated strips of detrusor with diltiazem (50 microM) inhibited the contractile responses to field stimulation (FS) and carbachol by 43 and 50%, respectively. Read More
Pharmacology 1999 Mar;58(3):113-9
Division of Basic and Pharmaceutical Sciences, Albany College of Pharmacy, and Stratton VA Medical Center, and Albany Medical College, Albany, N.Y., USA.
The effects of repetitive field stimulation (model of hyperrelexia) on the responses of isolated strips of rabbit urinary bladder to FS and carbachol were evaluated under a variety of incubation conditions. Compared to control conditions, 2 h of repetitive FS in normal, oxygenated Tyrode's solution followed by incubation for 1 h with no stimulation resulted in a 50% decrease in contractile response to FS and a 30% decrease in the response to carbachol. Incubation in the absence of O2 and glucose was used as an in vitro model for ischemia. Read More