Search Our Scientific Publications & Authors

Publications
find publications by category +
Translate page:

Synthesis of N-acetylneuraminyl-alpha 2,3(6)lactose-malate dehydrogenase conjugate for detecting sialic acid terminal groups on glycoproteins via homogeneous lectin-based enzyme-linked binding assay.

Authors:
X Guo M E Meyerhoff

Appl Biochem Biotechnol 1997 Oct-Nov;68(1-2):41-56

Department of Chemistry, University of Michigan, Ann Arbor 48109, USA.

An N-acetylneuraminyl-alpha 2,3(6)lactose-malate dehydrogenase (MDH-Lac-Neu5Ac) conjugate is prepared via an isothiocyanate conjugation method using a p-aminophenethylamino derivative of sialyllactose. The newly synthesized conjugate can be utilized as a reagent in a novel homogeneous lectin-based, enzyme-linked, competitive binding assay (1-3) for probing the specific carbohydrate structure and content of intact glycoproteins. The enzymatic activity of the MDH-Lac-Neu5Ac conjugate is shown to be significantly inhibited (35%) by sialic acid-binding lectin, Limax flavus agglutinin (LFA), and this inhibition is reversed by mucin, a glycoprotein possessing sialic acid terminals. The asialo form of mucin, however, binds weakly to LFA, yielding no substantial increase in the MDH-Lac-Neu5Ac activity at comparable glycoprotein concentrations. Use of the newly synthesized conjugate in conjunction with LFA or other lectins capable of binding sialic acid may provide a rapid and convenient way to detect the presence and relative amount of sialic acid terminal groups within intact glycoprotein structures.

Download full-text PDF

 Source
http://dx.doi.org/10.1007/BF02785979DOI Listing
February 1998

Publication Analysis

Top Keywords

sialic acid
16
mdh-lac-neu5ac conjugate
8
homogeneous lectin-based
8
lectin-based enzyme-linked
8
newly synthesized
8
terminal groups
8
binding assay
8
acid terminal
8
synthesized conjugate
8
236lactose-malate dehydrogenase
8
n-acetylneuraminyl-alpha 236lactose-malate
8
conjugate
5
sialic
5
glycoprotein possessing
4
possessing sialic
4
reversed mucin
4
acid terminals
4
inhibition reversed
4
terminals asialo
4
lfa inhibition
4

Keyword Occurance

Similar Publications

Mechanical Activation of the β-Adrenergic Receptor by Meningococcus: A Historical and Future Perspective Analysis of How a Bacterial Probe Can Reveal Signalling Pathways in Endothelial Cells, and a Unique Mode of Receptor Activation Involving Its N-Terminal Glycan Chains.

Authors:
Stefano Marullo Mark G H Scott Hervé Enslen Mathieu Coureuil

Front Endocrinol (Lausanne) 2022 2;13:883568. Epub 2022 May 2.

Université de Paris, Institut-Necker-Enfants-Malades, INSERM U1151, CNRS UMR 8253, Paris, France.

More than 12 years have passed since the seminal observation that meningococcus, a pathogen causing epidemic meningitis in humans, occasionally associated with infectious vasculitis and septic shock, can promote the translocation of β-arrestins to the cell surface beneath bacterial colonies. The cellular receptor used by the pathogen to induce signalling in host cells and allowing it to open endothelial cell junctions and reach meninges was unknown. The involvement of β-arrestins, which are scaffolding proteins regulating G protein coupled receptor signalling and function, incited us to specifically investigate this class of receptors. Read More

View Article and Full-Text PDF
May 2022
Similar Publications

PHGDH heterogeneity potentiates cancer cell dissemination and metastasis.

Authors:
Matteo Rossi Patricia Altea-Manzano Margherita Demicco Ginevra Doglioni Laura Bornes Marina Fukano Anke Vandekeere Alejandro M Cuadros Juan Fernández-García Carla Riera-Domingo Cristina Jauset Mélanie Planque H Furkan Alkan David Nittner Dongmei Zuo Lindsay A Broadfield Sweta Parik Antonino Alejandro Pane Francesca Rizzollo Gianmarco Rinaldi Tao Zhang Shao Thing Teoh Arin B Aurora Panagiotis Karras Ines Vermeire Dorien Broekaert Joke Van Elsen Maximilian M L Knott Martin F Orth Sofie Demeyer

Nature 2022 May 18. Epub 2022 May 18.

Laboratory for Molecular Biology of Leukemia, VIB-KU Leuven, Leuven, Belgium.

Cancer metastasis requires the transient activation of cellular programs enabling dissemination and seeding in distant organs. Genetic, transcriptional and translational heterogeneity contributes to this dynamic process. Metabolic heterogeneity has also been observed, yet its role in cancer progression is less explored. Read More

View Article and Full-Text PDF
May 2022
Similar Publications

O-acetylesterase activity of Bifidobacterium bifidum sialidase facilities the liberation of sialic acid and encourages the proliferation of sialic acid scavenging Bifidobacterium breve.

Authors:
Tatsunari Yokoi Keita Nishiyama Yuka Kushida Kazuya Uribayashi Takahiro Kunihara Rika Fujimoto Yuji Yamamoto Masahiro Ito Tsuyoshi Miki Takeshi Haneda Takao Mukai Nobuhiko Okada

Environ Microbiol Rep 2022 May 17. Epub 2022 May 17.

Department of Microbiology, School of Pharmacy, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.

Bifidobacterium bifidum possesses two extracellular sialidases (SiaBb1 and SiaBb2) that release free sialic acid from mucin sialoglycans, which can be utilized via cross-feeding by Bifidobacterium breve that, otherwise, is prevented from utilizing this nutrient source. Modification of sialic acids with O-acetyl esters is known to protect mucin glycans from degradation by bacterial sialidases. Compared to SiaBb2, SiaBb1 has an additional O-acetylesterase (Est) domain. Read More

View Article and Full-Text PDF
May 2022
Similar Publications

Exploring the Candidate Terminal Glycan Profile in Neural Regeneration of the Sea Urchin , Using Lectin Blotting and Mass Spectrometry.

Authors:
Ramiz Demir Umut Şahar Remziye Deveci

Biol Bull 2022 Apr 9;242(2):118-126. Epub 2022 Mar 9.

AbstractGlycans are expressed as conjugates of glycoproteins, glycolipids, and proteoglycans. The huge diversity of glycans on glycoconjugates contributes to many biological processes, from glycan-based molecular recognition to developmental events, such as regeneration in the nervous system. Echinoderms, which have a close phylogenetic relationship with chordates, are an important group of marine invertebrates for body regeneration. Read More

View Article and Full-Text PDF
April 2022
Similar Publications

The role of the mucin-glycan foraging in the communication between the gut and the brain.

Authors:
Erika Coletto Dimitrios Latousakis Matthew G Pontifex Emmanuelle H Crost Laura Vaux Estella Perez Santamarina Andrew Goldson Arlaine Brion Mohammad K Hajihosseini David Vauzour George M Savva Nathalie Juge

Gut Microbes 2022 Jan-Dec;14(1):2073784

Gut Microbes and Health Institute Strategic Programme, Quadram Institute Bioscience, Norwich NR4 7UQ, UK.

is a prevalent member of the human gut microbiota, which is over-represented in inflammatory bowel disease and neurological disorders. We previously showed that the ability of to forage on mucins is strain-dependent and associated with sialic acid metabolism. Here, we showed that mice monocolonized with ATCC 29149 (-mice) display changes in major sialic acid derivatives in their cecum content, blood, and brain, which is accompanied by a significant decrease in the percentage of sialylated residues in intestinal mucins relative to germ-free (GF) mice. Read More

View Article and Full-Text PDF
May 2022
Similar Publications
}