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Methods Mol Biol 2022 ;2527:127-132

University of São Paulo/Center for Nuclear Energy in Agriculture-Plant Biotechnology Lab, Piracicaba, SP, Brazil.

Somatic embryogenesis has been obtained in many citrus cultivars; however, the efficiency of the system is genotype dependent and culture synchronization is important to reach more efficient systems. In this chapter we present a detailed protocol of somatic embryogenesis induction from nucellar tissue and the use of an alternative method of callus sieving for culture synchronization and embryo production. This is a simple method which can also be evaluated for other species aiming at better culture efficiency and somatic embryo production. Read More

STAR Protoc 2022 Sep 31;3(3):101579. Epub 2022 Jul 31.

Mechanisms of Protein Biogenesis, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.

Quantifying tRNAs is crucial for understanding how they regulate mRNA translation but is hampered by their extensive sequence similarity and premature termination of reverse transcription at multiple modified nucleotides. Here, we describe the use of modification-induced misincorporation tRNA sequencing (mim-tRNAseq), which overcomes these limitations with optimized library construction and a comprehensive toolkit for data analysis and visualization. We outline algorithm improvements that enhance the efficiency and accuracy of read alignment and provide details on data analysis outputs using example datasets. Read More

Front Plant Sci 2022 19;13:957133. Epub 2022 Jul 19.

Key Laboratory of Agricultural Biotechnology, College of Life Sciences, Shihezi University, Shihezi, China.

Late embryogenesis abundant proteins (LEA) help adapt to adverse low-temperature environments. The , which encodes a membrane protein, was significantly up-regulated in response to low temperature stress. expressing SiLEA4 showed enhanced low-temperature tolerance, as evident from the significantly higher survival numbers and growth rates at low temperatures. Read More

3 Biotech 2022 Sep 28;12(9):194. Epub 2022 Jul 28.

Department of Biotechnology, Kakatiya University, Warangal, Telangana 506009 India.

A reliable and stable -mediated genetic transformation system for has been developed using cell suspension cultures derived from cotyledon explants. Cotyledon, attached cotyledon, and compound leaves were found to be suitable for the induction of callus among five different types of explants tested. The yellow friable callus derived from attached cotyledon was used to initiate suspension cultures in Suspension Culture Medium (SCM) which was supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) at 2. Read More

J Immunol Methods 2022 Jul 28;508:113325. Epub 2022 Jul 28.

School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT) Deemed to be University, Bhubaneswar 751024, Odisha, India; KIIT-Technology Business Incubator (KIIT-TBI), Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar 751024, Odisha, India. Electronic address:

Acinetobacter baumannii, a prominent pathogen responsible for chronic infections in the blood, urinary tract, and lungs, has a high mortality due to its virulence and limited preventive methods. The present study aims to characterize the pilus assembly protein of A. baumannii to offer leads for epitope-based vaccine development. Read More