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Clin Epigenetics 2022 Aug 13;14(1):101. Epub 2022 Aug 13.

Division of Cardiothoracic and Vascular Surgery, Sino-Swiss Heart-Lung Transplantation Institute, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Ave, Wuhan, 430030, Hubei, China.

Background: Vascular smooth muscle cell (VSMC) phenotype switching is critical for neointima formation, which is the major cause of restenosis after stenting or coronary artery bypass grafting. However, the epigenetic mechanisms regulating phenotype switching of VSMCs, especially histone methylation, are not well understood. As a main component of histone lysine demethylases, Jumonji demethylases might be involved in VSMC phenotype switching and neointima formation. Read More

J Mol Diagn 2022 Aug 10. Epub 2022 Aug 10.

AP-HP, Henri Mondor University Hospital, Hematology and Immunology Department, F-94010 Creteil, France; Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France. Electronic address:

Several fusion genes such as BCR::ABL1, FIP1L1::PDGFRA and PML::RARA are now efficiently targeted by specific therapies in leukemic patients. Although these therapies have significantly improved patient outcomes, leukemia relapse and progression remain a clinical concern. Most myeloid next-generation sequencing (NGS) panels don't detect or quantify these fusions. Read More

J Thromb Haemost 2022 Aug 12. Epub 2022 Aug 12.

Department of Medicine, Stony Brook University, Stony Brook, NY, USA.

Background: Developmental ontogeny of neonatal thrombopoiesis retains characteristics that are distinct from adult although molecular mechanisms remain unestablished METHODS: We applied multiparameter quantitative platelet responses with integrated ribosome profiling/transcriptomic studies to better define gene/pathway perturbations regulating the neonatal-to-adult transition. A bioinformatics pipeline was developed to identify stable, neonatal-restricted platelet biomarkers for clinical application RESULTS: Cord blood (CB) platelets retained the capacity for linear agonist-receptor coupling linked to phosphatidylserine exposure (PS) and α-granule release, although a restricted block in cross-agonist activation pathways was evident. Functional immaturity of synergistic signaling pathways was due to younger ontogenetic age and singular underdevelopment of the protein secretory gene network, with reciprocal expansion of developmental pathways (E2F, G2M checkpoint, c-Myc) important for megakaryocytopoiesis. Read More

Anal Biochem 2022 Aug 9:114841. Epub 2022 Aug 9.

Department of Biochemistry, University of Western Ontario London, Ontario, N6G 3C3, Canada.

Filter paper provides an excellent matrix for retention of proteins containing a cellulose binding domain. To use this capability for manipulating recombinant fusion proteins, binding and elution parameters were explored and procedures developed for small scale purification, modification and assay. Proteins were tagged with the cellulose binding domain from the Clostridium thermocellum CipB gene via a cleavable linker. Read More

Am J Transl Res 2022 15;14(7):4990-5002. Epub 2022 Jul 15.

Department of Biological Therapy, Eastern Hepatobiliary Surgery Hospital Shanghai 201805, China.

Background: The incidence of biliary system cancer is higher in the Chinese population than in the West. The overall prognosis of gallbladder cancer and cholangiocarcinoma is poor, and the current treatment is limited. In order to explore the pathogenesis of biliary tract cancers and potential targeted therapies, we mapped the mutation landscape of biliary tract cancer in the Chinese population and analyzed the molecular mechanism related to prognosis. Read More