Pubfacts - Scientific Publication Data
  • Categories
  • |
  • Journals
  • |
  • Authors
  • Login
  • Categories
  • Journals

Search Our Scientific Publications & Authors

Publications
  • Publications
  • Authors
find publications by category +
Translate page:

Characterization and expression of prohibitin during the mexican bean weevil (Zabrotes subfasciatus, Boheman, 1833) larvae development.

Authors:
Diana Villegas-Coronado Ana María Guzman-Partida Emmanuel Aispuro-Hernandez Luz Vazquez-Moreno José Ángel Huerta-Ocampo José Andre-I Sarabia-Sainz Nayelli Guadalupe Teran-Saavedra Christian Minjarez-Osorio Reina Castro-Longoria Amir Maldonado Irlanda Lagarda-Diaz

Comp Biochem Physiol B Biochem Mol Biol 2022 Oct-Dec;262:110770. Epub 2022 May 27.

CONACyT- Departamento de Física, Universidad de Sonora, Hermosillo, 83000, Mexico. Electronic address:

Prohibitin (PHB) is a highly conserved eukaryotic protein complex involved in multiple cellular processes. In insects, PHB has been identified as a potential target protein to insecticidal molecules acting as a receptor of PF2 insecticidal lectin in the midgut of Zabrotes subfasciatus larvae (bean pest) and Cry protein of Bacillus thuringiensis in Leptinotarsa decemlineata (Colorado potato beetle). This study aimed to characterize the structural features of Z. subfasciatus prohibitin (ZsPHB) by homology modeling and evaluate its expression and tissue localization at different stages of larval development both at the transcript and protein levels. The samples were collected from eggs and larvae of different developmental stages. The immunodetection of ZsPHB was done with anti-PHB1 and confirmed by LC-MS/MS analysis. Gene expression analysis of ZsPHB1 and ZsPHB2 was performed by RT-qPCR, and immunohistochemistry with FITC-labeled anti-PHB1. Results showed that ZsPHBs exhibit distinctive characteristics of the SPFH protein superfamily. The transcript levels suggest a coordinated expression of ZsPHB1 and ZsPHB2 genes, while ZsPHB1 was detected in soluble protein extracts depending on the stage of development. Histological examination showed ZsPHB1 is present in all larval tissues, with an intense fluorescence signal observed at the gut. These results suggest a physiologically important role of PHB during Z. subfasciatus development and show its regulation occurs at the transcriptional and post-transcriptional levels. This is the first characterization of PHB in Z. subfasciatus.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpb.2022.110770DOI Listing
August 2022

Publication Analysis

Top Keywords

zabrotes subfasciatus
8
zsphb1 zsphb2
8
phb subfasciatus
8
protein
6
subfasciatus
5
larvae developmental
4
developmental stages
4
stages immunodetection
4
immunodetection zsphb
4
zsphb anti-phb1
4
anti-phb1 confirmed
4
confirmed lc-ms/ms
4
lc-ms/ms analysis
4
characterization phb
4
collected eggs
4
analysis gene
4
gene expression
4
expression analysis
4
zsphb1
4
analysis zsphb1
4

Keyword Occurance

Similar Publications

Introgression of bruchid ( resistance into small red common bean () background and validation of the BRU_00261 (snpPV0007) resistance marker.

Authors:
Shiferaw Girsil Tigist Bodo Raatz Amelework Assefa Rob Melis Julia Sibiya Gemechu Keneni Clare Mukankusi Berhanu Fenta Selamawit Ketema Dagmawit Tsegaye

Plant Breed 2021 Dec 9;140(6):1081-1089. Epub 2021 Oct 9.

Ethiopian Institute of Agricultural Research Melkassa Agricultural Research Centre Adama Ethiopia.

Bruchids are a major storage pest of common bean. Genetic resistance is a suitable method to avoid grain losses during storage. The objective of the study was to introgress the arcelin-based resistance locus into selected advanced breeding line and to validate the molecular marker BRU_00261. Read More

View Article and Full-Text PDF
December 2021
Similar Publications

Characterization and expression of prohibitin during the mexican bean weevil (Zabrotes subfasciatus, Boheman, 1833) larvae development.

Authors:
Diana Villegas-Coronado Ana María Guzman-Partida Emmanuel Aispuro-Hernandez Luz Vazquez-Moreno José Ángel Huerta-Ocampo José Andre-I Sarabia-Sainz Nayelli Guadalupe Teran-Saavedra Christian Minjarez-Osorio Reina Castro-Longoria Amir Maldonado Irlanda Lagarda-Diaz

Comp Biochem Physiol B Biochem Mol Biol 2022 Oct-Dec;262:110770. Epub 2022 May 27.

CONACyT- Departamento de Física, Universidad de Sonora, Hermosillo, 83000, Mexico. Electronic address:

Prohibitin (PHB) is a highly conserved eukaryotic protein complex involved in multiple cellular processes. In insects, PHB has been identified as a potential target protein to insecticidal molecules acting as a receptor of PF2 insecticidal lectin in the midgut of Zabrotes subfasciatus larvae (bean pest) and Cry protein of Bacillus thuringiensis in Leptinotarsa decemlineata (Colorado potato beetle). This study aimed to characterize the structural features of Z. Read More

View Article and Full-Text PDF
August 2022
Similar Publications

Recruitment of lysosomal cathepsins B, L and D as digestive enzymes in Coleoptera.

Authors:
Carlos P Silva Renata O Dias Vanessa Bernardes Ignacio G Barroso Christiane Cardoso Clelia Ferreira Walter R Terra

Insect Mol Biol 2022 04 28;31(2):225-240. Epub 2021 Dec 28.

Departamento de Bioquímica, Instituto de Química, Universidade de São, São Paulo, Brazil.

The recruitment of the lysosomal cathepsins B (CAB), L (CAL) and D (CAD) as luminal digestive enzymes was investigated in 3 species of beetles. Gene expression was determined by RNA-seq in different regions of the midgut and in the carcasses from the transcriptomes of Dermestes maculatus, Tenebrio molitor and Zabrotes subfasciatus. These data together with phylogenetic analyses, allowed us to identify the sequences of the gene coding for digestive and lysosomal CABs, CADs and CALs in T. Read More

View Article and Full-Text PDF
April 2022
Similar Publications

Chemical composition and insecticidal activity of the essential oils of Piper marginatum , Piper callosum and Vitex agnus-castus.

Authors:
Vanessa F S Ayres Midiã R Oliveira Edson L L Baldin Geone M Corrêa Anderson C Guimarães Renata Takeara

An Acad Bras Cienc 2021 19;93(3):e20200616. Epub 2021 Jul 19.

Universidade Federal do Amazonas, Instituto de Ciências Exatas e Tecnologia, Rua Nossa Senhora do Rosário, 3863, 69103-128 Itacoatiara, AM, Brazil.

During grain storage, a considerable amount of product is lost because of insects, such as Zabrotes subfasciatus. Currently, to mitigate these risks, studies are searching for plants with potential for the control of agricultural pests, also known as botanical insecticides. In this study, the fumigant toxicity of the essential oils of Piper callosum (PC-EO), Piper marginatum (PM-EO) and Vitex agnus-castus (VA-EO) against Zabrotes subfasciatus was investigated. Read More

View Article and Full-Text PDF
July 2021
Similar Publications

Multigenerational experimental simulation of climate change on an economically important insect pest.

Authors:
David Schneider Alejandra G Ramos Alex Córdoba-Aguilar

Ecol Evol 2020 Dec 27;10(23):12893-12909. Epub 2020 Oct 27.

Departamento de Ecología Evolutiva Instituto de Ecología Universidad Nacional Autónoma de México México Mexico.

Long-term multigenerational experimental simulations of climate change on insect pests of economically and socially important crops are crucial to anticipate challenges for feeding humanity in the not-so-far future. Mexican bean weevil , is a worldwide pest that attacks the common bean seeds, in crops and storage. We designed a long term (i. Read More

View Article and Full-Text PDF
December 2020
Similar Publications
}
© 2022 PubFacts.
  • About PubFacts
  • Privacy Policy
  • Sitemap