Search Our Scientific Publications & Authors

Publications
find publications by category +
Translate page:

Investigating the Interactions of Glioma Stem Cells in the Perivascular Niche at Single-Cell Resolution using a Microfluidic Tumor Microenvironment Model.

Authors:
Emmanuela A Adjei-Sowah Samantha A O'Connor Jaimeson Veldhuizen Costanza Lo Cascio Christopher Plaisier Shwetal Mehta Mehdi Nikkhah

Adv Sci (Weinh) 2022 May 26:e2201436. Epub 2022 May 26.

School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, 85287-9709, USA.

The perivascular niche (PVN) is a glioblastoma tumor microenvironment (TME) that serves as a safe haven for glioma stem cells (GSCs), and acts as a reservoir that inevitably leads to tumor recurrence. Understanding cellular interactions in the PVN that drive GSC treatment resistance and stemness is crucial to develop lasting therapies for glioblastoma. The limitations of in vivo models and in vitro assays have led to critical knowledge gaps regarding the influence of various cell types in the PVN on GSCs behavior. This study developed an organotypic triculture microfluidic model as a means to recapitulate the PVN and study its impact on GSCs. This triculture platform, comprised of endothelial cells (ECs), astrocytes, and GSCs, is used to investigate GSC invasion, proliferation and stemness. Both ECs and astrocytes significantly increased invasiveness of GSCs. This study futher identified 15 ligand-receptor pairs using single-cell RNAseq with putative chemotactic mechanisms of GSCs, where the receptor is up-regulated in GSCs and the diffusible ligand is expressed in either astrocytes or ECs. Notably, the ligand-receptor pair SAA1-FPR1 is demonstrated to be involved in chemotactic invasion of GSCs toward PVN. The novel triculture platform presented herein can be used for therapeutic development and discovery of molecular mechanisms driving GSC biology.

Download full-text PDF

 Source
http://dx.doi.org/10.1002/advs.202201436DOI Listing
May 2022

Publication Analysis

Top Keywords

tumor microenvironment
8
triculture platform
8
ecs astrocytes
8
stem cells
8
perivascular niche
8
glioma stem
8
gscs
8
pvn
5
study developed
4
pvn gscs
4
gscs behavior
4
behavior study
4
investigating interactions
4
astrocytes ecs
4
model recapitulate
4
expressed astrocytes
4
developed organotypic
4
cell types
4
organotypic triculture
4
triculture microfluidic
4

Keyword Occurance

Similar Publications

Emerging strategies for the improvement of chemotherapy in bladder cancer: Current knowledge and future perspectives.

Authors:
Sen Liu Xu Chen Tianxin Lin

J Adv Res 2022 Jul 24;39:187-202. Epub 2021 Nov 24.

Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China; Guangdong Provincial Clinical Research Center for Urological Diseases, China. Electronic address:

Background: Chemotherapy is a first-line treatment for advanced and metastatic bladder cancer, but the unsatisfactory objective response rate to this treatment yields poor 5-year patient survival. Only PD-1/PD-L1-based immune checkpoint inhibitors, FGFR3 inhibitors and antibody-drug conjugates are approved by the FDA to be used in bladder cancer, mainly for platinum-refractory or platinum-ineligible locally advanced or metastatic urothelial carcinoma. Emerging studies indicate that the combination of targeted therapy and chemotherapy shows better efficacy than targeted therapy or chemotherapy alone. Read More

View Article and Full-Text PDF
July 2022
Similar Publications

Opportunities and challenges of bi-specific antibodies.

Authors:
Aina Segués Shuyu Huang Alice Sijts Pedro Berraondo Dietmar M Zaiss

Int Rev Cell Mol Biol 2022 30;369:45-70. Epub 2022 May 30.

Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom; Department of Immune Medicine, University Regensburg, Regensburg, Germany; Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany; Institute of Pathology, University Regensburg, Regensburg, Germany. Electronic address:

The recent clinical approval of different Bi-specific antibodies (BsAbs) has revealed the great therapeutic potential of this novel class of biologicals. For example, the bispecific T-cell engager (BiTE), Blinatumomab, demonstrated the unique capacity of BsAbs to link T-cells with tumor cells, inducing targeted tumor cell removal. Additionally, Amivantamab, recognizing the EGFR and cMet in cis, revealed a substantial improvement of therapeutic efficacy by concomitantly targeting two tumor antigens. Read More

View Article and Full-Text PDF
May 2022
Similar Publications

Overcoming the limitations of cytokines to improve cancer therapy.

Authors:
Claudia Augusta Di Trani Assunta Cirella Leire Arrizabalaga Myriam Fernandez-Sendin Angela Bella Fernando Aranda Ignacio Melero Pedro Berraondo

Int Rev Cell Mol Biol 2022 30;369:107-141. Epub 2022 May 30.

Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IDISNA), Pamplona, Spain; Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Madrid, Spain. Electronic address:

Cytokines are pleiotropic soluble proteins used by immune cells to orchestrate a coordinated response against pathogens and malignancies. In cancer immunotherapy, cytokine-based drugs can be developed potentiating pro-inflammatory cytokines or blocking immunosuppressive cytokines. However, the complexity of the mechanisms of action of cytokines requires the use of biotechnological strategies to minimize systemic toxicity, while potentiating the antitumor response. Read More

View Article and Full-Text PDF
May 2022
Similar Publications

Electroacupuncture promotes apoptosis and inhibits axonogenesis by activating p75 neurotrophin receptor for triple-negative breast xenograft in mice.

Authors:
Yehong Tian Xiaowei Qiu Xuewei Qi Zhenzhen Dong Jianxin Zhao Jinchang Huang Xin Jiang

J Chem Neuroanat 2022 Jun 28:102133. Epub 2022 Jun 28.

The Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China; Institute of acupuncture and moxibustion in cancer care, Beijing University of Chinese Medicine. Electronic address:

Purpose: The aim of this study was to investigate the anti-tumor effect of electroacupuncture (EA) on mice bearing breast tumors by regulating p75 neurotrophin receptor (p75NTR) and remodelling intratumoral innervation.

Methods: Female BALB/c mice were implanted with 4T1 breast tumor cells to establish a murine mammary cancer model. Tumor volume and weight were measured to evaluate tumor growth. Read More

View Article and Full-Text PDF
June 2022
Similar Publications

3d oxidized alginate-porcine liver acellular collagen droplets for tumor microenvironment mimicking.

Authors:
Yanan Li Lingyun He Jiamin Chen Jinfeng Wang Shujing Zhao Xingxing Liu Xiaoling Guo Ying Wu Xian Shen Chao Li

Int J Biol Macromol 2022 Jun 28. Epub 2022 Jun 28.

The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109 Xueyuan West Road, Wenzhou 325027, PR China. Electronic address:

The traditional 2d culture has been proved inferior to reproduce the subtle interaction between cell-to-cell and cell-to-extracellular matrix (ECM) in tumor microenvironment (TME) and collagen in ECM contributes to various malignancies of tumors. Hence, the 3d model contained with collagen may overcome the shortcomings of 2d culture. In this study, the in vitro TME mimicking matrix was prepared by coupling porcine liver-derived collagen (COL) and the dialdehyde group of partially oxidized alginate (OA), namely OA-COL, and the 3d OA-COL droplets were polymerized by divalent calcium ions. Read More

View Article and Full-Text PDF
June 2022
Similar Publications
}