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The development of a colorimetric biosensing assay for the detection of Helicobacter pylori in feces.

Authors:
Yu Fei Rong Fang Lina Xiao Yuqing Zhang Ke Fan Yundi Jiang Silu Lei Rui Xu Dailan Yang Yan Ye Shibing Xiang Ping Wang Chen Zhou Tian Tang

Anal Biochem 2022 08 18;651:114737. Epub 2022 May 18.

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, Sichuan, PR China; West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, 610041, Sichuan, PR China. Electronic address:

As Helicobacter pylori (H. pylori) is closely related to the occurrence of gastric diseases such as chronic gastritis, peptic ulcer, and gastric cancer, early detection of H. pylori is an urgent need. In this study, oligonucleotide probes conjugated with gold nanoparticles (AuNPs) were used in combination with H. pylori-specific aptamers for the rapid detection of H. pylori in stool samples, which converted the method of detection from proteins to nucleic acids. Therefore, qualitative detection of H. pylori can be achieved by observing color changes through the aggregation (red to purple) or deaggregation (purple to red) of AuNPs, and further quantitative detection can be achieved through UV spectrometry. The detection limit of the colorimetric biosensing method is 25 CFU/mL (S/N = 3), which is favorably comparable to other reported detection methods. Compared with the existing detection methods for H. pylori, this colorimetric biosensing method has no limitations to the test subjects. All these features render the colorimetric biosensing assay a promising method for the clinical field detection of H. pylori.

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http://dx.doi.org/10.1016/j.ab.2022.114737DOI Listing
August 2022

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