Search Our Scientific Publications & Authors

Publications
find publications by category +
Translate page:

COMPARATIVE STUDY ON THE IMPACT OF DIFFERENT IMMOBILIZATION METHODS ON MUSCLE INTEGRITY AND STRESS INDICATORS IN BROOK TROUT ().

Authors:
Benjamin Lamglait Stéphane Lair

J Zoo Wildl Med 2022 Mar;53(1):116-125

Centre québécois sur la santé des animaux sauvages/Canadian Wildlife Health Cooperative, Faculté de médecine vétérinaire, Université de Montréal, Saint Hyacinthe, Quebec J2S 2M2, Canada,

Chemical sedation of fish is often used during scientific research to reduce stress and risk of injury. Electric fish handling gloves (EFHG) have been proposed as a no-residue alternative to chemical immobilization. However, the impact of handling fish with EFHG on their physiology remains relatively poorly studied. Stress markers (plasma cortisol and lactate) and the impact on muscular integrity (plasma creatine kinase and histopathology of skeletal muscles) were assessed in brook trout () subjected to one of three immobilization methods (manual restraint, EFHG [10-16 mV], and buffered 60 mg/L tricaine methanesulfonate [MS-222] immersion anesthesia) for 1- and 5-min restraints. Plasma lactate levels were significantly higher 2 h postrestraint for all experimental groups (+242%; < 0.001), but the magnitude of these increases was significantly lower in the MS-222 groups ( ≤ 0.0002). Plasma creatine kinase concentrations significantly increased 2 h postrestraint for the EFHG groups (+92%, ≤ 0.0061), and this increase was significantly higher than in the MS-222 group for the 1-min restraint ( = 0.0031). Although there was no significant difference between the restraining methods tested at all time points for plasma cortisol, the EFHG and manual restraint groups showed a linear and statistically significant increase after the initial 5-min restraint, whereas all other groups presented an expected bell-shaped profile with a plasma peak 1 h after the initial restraint. One fish in the manual restraint group died during the 5-min restraint, and two fish from the EFHG group were euthanized at the end of this protocol because of buoyancy anomalies. All other fish from the 5-min restraint group developed cutaneous saprolegniasis 3 wk after the experiments. The evaluation of the safety of EFHG in brook trout showed similar impacts on the variables tested compared with manual restraint for 1- and 5-min immobilizations.

Download full-text PDF

 Source
http://dx.doi.org/10.1638/2021-0085DOI Listing
March 2022

Publication Analysis

Top Keywords

manual restraint
16
brook trout
12
5-min restraint
12
restraint
9
fish efhg
8
restraint groups
8
plasma cortisol
8
plasma creatine
8
creatine kinase
8
restraint fish
8
restraint group
8
immobilization methods
8
efhg
7
fish
6
plasma
6
5-min
5
groups
5
5-min restraints
4
increased postrestraint
4
[ms-222] immersion
4

Keyword Occurance

Similar Publications

Factors affecting inter-rater agreement in human classification of eye movements: a comparison of three datasets.

Authors:
Lee Friedman Vladyslav Prokopenko Shagen Djanian Dmytro Katrychuk Oleg V Komogortsev

Behav Res Methods 2022 Apr 11. Epub 2022 Apr 11.

Derrick M5, Department of Computer Science, Texas State University, 601 University Drive, San Marcos, Texas, 78640, USA.

Manual classification of eye-movements is used in research and as a basis for comparison with automatic algorithms in the development phase. However, human classification will not be useful if it is unreliable and unrepeatable. Therefore, it is important to know what factors might influence and enhance the accuracy and reliability of human classification of eye-movements. Read More

View Article and Full-Text PDF
April 2022
Similar Publications

COMPARATIVE STUDY ON THE IMPACT OF DIFFERENT IMMOBILIZATION METHODS ON MUSCLE INTEGRITY AND STRESS INDICATORS IN BROOK TROUT ().

Authors:
Benjamin Lamglait Stéphane Lair

J Zoo Wildl Med 2022 Mar;53(1):116-125

Centre québécois sur la santé des animaux sauvages/Canadian Wildlife Health Cooperative, Faculté de médecine vétérinaire, Université de Montréal, Saint Hyacinthe, Quebec J2S 2M2, Canada,

Chemical sedation of fish is often used during scientific research to reduce stress and risk of injury. Electric fish handling gloves (EFHG) have been proposed as a no-residue alternative to chemical immobilization. However, the impact of handling fish with EFHG on their physiology remains relatively poorly studied. Read More

View Article and Full-Text PDF
March 2022
Similar Publications

Most students have experience making radiographs prior to veterinary school but have limited radiation safety training.

Authors:
Monica Jensen Joanne Hewson Heather Chalmers Andria Jones-Bitton Ameet Singh

Vet Radiol Ultrasound 2022 Mar 21;63(2):131-137. Epub 2022 Feb 21.

Department of Clinical Studies, Ontario Veterinary College, Guelph, Canada.

The use of radiation for the diagnosis of various medical conditions is ubiquitous in veterinary medicine, and manual restraint of a patient during X-ray exposure has become commonplace. Many prospective veterinary students have been involved with radiographs in general practice prior to veterinary school. Their experiences and level of safety training are largely unknown. Read More

View Article and Full-Text PDF
March 2022
Similar Publications

Validation of Medicinal Leeches () as a Non-invasive Blood Sampling Tool for Hematology and Biochemistry Profiling in Mammals.

Authors:
Pavel Kvapil Oldřich Tomášek Eva Bártová Mojca Harej Marjan Kastelic Tit Primožič Nikola Kašpárková Jožko Račnik

Front Vet Sci 2022 4;9:831836. Epub 2022 Feb 4.

Faculty of Veterinary Medicine, Institute of Poultry, Birds, Small Mammals and Reptiles, University of Ljubljana, Ljubljana, Slovenia.

Blood sampling is a challenging procedure in many captive animals. Although manual restraint or anesthesia are usually possible, they entail intense stress and a high risk of injuries or organ failure. Blood sampling using medicinal leeches () represents a promising non-invasive alternative to venipuncture; however, leech blood meal was to date used only for qualitative analyses such as genetic or serological screenings. Read More

View Article and Full-Text PDF
February 2022
Similar Publications

Use of Blood Lactate in Assessment of Manual Capture Techniques of Zoo-Housed Crocodilians.

Authors:
Holly Grace Molinaro Gen S Anderson Lauren Gruny Emily S Sperou Darryl J Heard

Animals (Basel) 2022 Feb 8;12(3). Epub 2022 Feb 8.

Department of Comparative Diagnostic and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.

Species-specific welfare indicators are important in promoting positive welfare for zoo animals. Reptiles are a notoriously understudied group in regards to behavior, welfare needs, and husbandry requirements. Using opportunistically obtained samples, we evaluated how blood lactate is affected by variation in manual capture and restraint in crocodilians. Read More

View Article and Full-Text PDF
February 2022
Similar Publications
}