In this work, we used a thread-paper microfluidic device (μTPAD) system, where a threaded part for the handling of the whole blood samples and a paper part for the reaction of plasma with immobilized bioreagents integrated into woman pad as a wearable sensing device namely as smart women pad. The μTPAD as a wearable smart woman pad is developed for the detection of pH and urea in mensuration blood as real samples. This combined device was constructed to cover the elements required, that is, separation of red blood cell, conditioning, analyte reaction, and colorimetric detection.