Search Our Scientific Publications & Authors

Publications
find publications by category +
Translate page:

The probiotic properties and potential of vaginal Lactobacillus spp. isolated from healthy women against some vaginal pathogens.

Authors:
A Asadi V Lohrasbi M Abdi S Mirkalantari M Esghaei M Kashanian M Oshaghi M Talebi

Lett Appl Microbiol 2022 May 11;74(5):752-764. Epub 2022 Feb 11.

Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

During the last decade, probiotic research has progressed considerably and significant advances have been made in the selection and characterization of specific probiotic strains. The most studied probiotics belong to the genus Lactobacillus. In this study, 80 Lactobacillus spp. isolated from healthy women tolerated low pH and were able to grow in the presence of bile salts. RAPD PCR technique resulted in the identification of 38 different types. These isolates were then evaluated based on adhesion capacity, antibiotic susceptibility and tolerance in simulated gastrointestinal tract. Species-specific PCR and detection of bacteriocin-related genes were also surveyed. Among the isolates, five strains-Lacticaseibacillus rhamnosus NO21, Lacticaseibacillus casei NO1, Lactiplantibacillus plantarum NO4, Lactobacillus acidophilus NO7 and Lactobacillus gasseri NO38-presented acceptable antibiotic susceptibility pattern. Further analysis showed antimicrobial activity of Lacticaseibacillus culture against various bacterial pathogens and real-time PCR showed all five strains were able to prevent the colonization of bacterial pathogens. All five selected strains produced organic acids, hydrogen peroxide and were resistant to the spermicide. In addition, they lacked haemolytic activity with the ability of hydrophobicity, auto-aggregation and co-aggregation with pathogens. These results suggest that the vaginal microbiome could be a good source for the isolation of probiotics and the strains of this study may be considered as good probiotic candidates.

Download full-text PDF

 Source
http://dx.doi.org/10.1111/lam.13660DOI Listing
May 2022

Publication Analysis

Top Keywords

antibiotic susceptibility
8
lactobacillus spp
8
bacterial pathogens
8
healthy women
8
isolated healthy
8
spp isolated
8
lactobacillus
5
antimicrobial activity
4
activity lacticaseibacillus
4
plantarum no4
4
no4 lactobacillus
4
lactobacillus acidophilus
4
acidophilus no7
4
no7 lactobacillus
4
lactobacillus gasseri
4
susceptibility pattern
4
analysis antimicrobial
4
gasseri no38-presented
4
no1 lactiplantibacillus
4
no38-presented acceptable
4

Keyword Occurance

Similar Publications

A high level of antibiotic resistance in and isolates from street water sold in Mozambique, associated with the prevalence of extended-spectrum and AmpC ß-lactamases.

Authors:
Acácio Salamandane Manuel Malfeito-Ferreira Luísa Brito

J Environ Sci Health B 2022 May 23:1-7. Epub 2022 May 23.

Faculdade de Ciências de Saúde, Universidade Lúrio, Nampula, Mozambique.

This study aims to evaluate the resistance profile and the prevalence of antibiotic resistance genes in 30 isolates of spp. and spp. recovered from water sold in the streets of Maputo. Read More

View Article and Full-Text PDF
May 2022
Similar Publications

[Peritoneal dialysis-associated peritonitis caused by Rhizobium species - a case report].

Authors:
Allan Zhao Anna Andersson Oscar Backrud Olof Heimbürger

Lakartidningen 2022 May 20;119. Epub 2022 May 20.

docent, överläkare, Karolinska institutet; ME njurmedicin, Karolinska universitetssjukhuset.

Peritonitis is a serious complication associated with high morbidity and mortality in patients with peritoneal dialysis (PD). We present a case of PD-associated peritonitis caused by the unusual pathogen Rhizobium. After therapy failure and bacterial growth despite treatment with vancomycin and tobramycin, the treatment was changed to meropenem intravenously and ciprofloxacin intraperitoneally according to antimicrobial susceptibility testing. Read More

View Article and Full-Text PDF
May 2022
Similar Publications

Personalized antibiograms for machine learning driven antibiotic selection.

Authors:
Conor K Corbin Lillian Sung Arhana Chattopadhyay Morteza Noshad Amy Chang Stanley Deresinksi Michael Baiocchi Jonathan H Chen

Commun Med (Lond) 2022 8;2:38. Epub 2022 Apr 8.

Center of Biomedical Informatics Research, Stanford University, Stanford, CA USA.

Background: The Centers for Disease Control and Prevention identify antibiotic prescribing stewardship as the most important action to combat increasing antibiotic resistance. Clinicians balance broad empiric antibiotic coverage vs. precision coverage targeting only the most likely pathogens. Read More

View Article and Full-Text PDF
April 2022
Similar Publications

Molecular and genetic characterization of emerging carbapenemase-producing strains from patients and hospital environments in Bangladesh.

Authors:
Refath Farzana Göte Swedberg Christian G Giske Badrul Hasan

Infect Prev Pract 2022 Jun 27;4(2):100215. Epub 2022 Apr 27.

Section for Infectious Diseases, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.

Background: Carbapenemase-producing multidrug-resistant (MDR) is a global health care problem. MDR has emerged as an important nosocomial pathogen, costing many lives worldwide including Bangladesh.

Aim: To investigate the detailed molecular epidemiology of carbapenem-resistant (CRAB) both from patients and the hospital environment, to shed light on genetic characteristics and transmission dynamics. Read More

View Article and Full-Text PDF
June 2022
Similar Publications

The Integration of Proteomics and Metabolomics Data Paving the Way for a Better Understanding of the Mechanisms Underlying Microbial Acquired Drug Resistance.

Authors:
Suereta Fortuin Nelson C Soares

Front Med (Lausanne) 2022 6;9:849838. Epub 2022 May 6.

College of Pharmacy, University of Sharjah, Sharjah, United Arab Emirates.

Due to an increase in the overuse of antimicrobials and accelerated incidence of drug resistant pathogens, antimicrobial resistance has become a global health threat. In particular, bacterial antimicrobial resistance, in both hospital and community acquired transmission, have been found to be the leading cause of death due to infectious diseases. Understanding the mechanisms of bacterial drug resistance is of clinical significance irrespective of hospital or community acquired since it plays an important role in the treatment strategy and controlling infectious diseases. Read More

View Article and Full-Text PDF
May 2022
Similar Publications
}