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Role of silver nanoparticles in fluorimetric determination of urea in urine samples.

Authors:
Muhammad Ismail Wang Xiangke Gerardo Cazzato Hassan Anwar Saleemi Ayub Khan Ahmed Ismail Muhammad Zahid Muhammad Farooq Khan

Spectrochim Acta A Mol Biomol Spectrosc 2022 Apr 11;271:120889. Epub 2022 Jan 11.

Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan.

Herein, an economical, analytical and sensitive method was established for the fluorometric determination of urea using freshly prepared silver nanoparticles (Ag-NPs) in real urine samples. The standard addition and second-order derivative methods were selected for the ongoing research work to eliminate the possible effect of interferences in a real environment. In this work, Ag-NPs were prepared by reducing silver nitrate salt in the presence of 1,3-di-(1H-imidazole-1-yl) -2-propanol (DIPO) in an aqueous medium. Urea in the urine samples was successfully determined through the complexation of Ag-NPs with urea molecules. The results revealed high percent recovery with ± RSD of urea in the three different urine samples, where percent recoveries by spectrofluorometric standard addition were 99.77 ± 3.4, 100.24 ± 5.1, 100.93 ± 2.8 and that is by the spectrofluorometric second-order derivative method were 103.57 ± 2.4, 101.8 ± 1.3, 98 ± 3.2, respectively. The successful application of these analytical methods in the spectrofluorometric determination of urea in urine samples can accumulate further addition in the effects and possible role of Ag-NPs in the determination of biological molecules in biological and non-biological samples in the scientific as well as clinical fields.

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http://dx.doi.org/10.1016/j.saa.2022.120889DOI Listing
April 2022

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