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Volatile Organic Compounds from MCCC 1K02966 with Multiple Modes against .

Authors:
Wen Chen Jinping Wang Dian Huang Wanli Cheng Zongze Shao Minmin Cai Longyu Zheng Ziniu Yu Jibin Zhang

Molecules 2021 Dec 24;27(1). Epub 2021 Dec 24.

State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Plant-parasitic nematodes cause severe losses to crop production and economies all over the world. MCCC 1K02966, a deep-sea bacterium, was obtained from the Southwest Indian Ocean and showed nematicidal and fumigant activities against in vitro. The nematicidal volatile organic compounds (VOCs) from the fermentation broth of MCCC 1K02966 were investigated further using solid-phase microextraction gas chromatography-mass spectrometry. Four VOCs, namely, pentane, 1-butanol, methyl thioacetate, and dimethyl disulfide, were identified in the fermentation broth. Among these VOCs, methyl thioacetate exhibited multiple nematicidal activities, including contact nematicidal, fumigant, and repellent activities against . Methyl thioacetate showed a significant contact nematicidal activity with 87.90% mortality at 0.01 mg/mL by 72 h, fumigant activity in mortality 91.10% at 1 mg/mL by 48 h, and repellent activity at 0.01-10 mg/mL. In addition, methyl thioacetate exhibited 80-100% egg-hatching inhibition on the 7th day over the range of 0.5 mg/mL to 5 mg/mL. These results showed that methyl thioacetate from MCCC 1K02966 control with multiple nematicidal modes and can be used as a potential biological control agent.

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http://dx.doi.org/10.3390/molecules27010103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747049PMC
December 2021

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