Plant-parasitic nematodes cause severe losses to crop production and economies all over the world. MCCC 1K02966, a deep-sea bacterium, was obtained from the Southwest Indian Ocean and showed nematicidal and fumigant activities against in vitro. The nematicidal volatile organic compounds (VOCs) from the fermentation broth of MCCC 1K02966 were investigated further using solid-phase microextraction gas chromatography-mass spectrometry.